Detection of protein ubiquitination

Journal of Visualized Experiments

Volumn , Issue 30, 2009, Pages

  Choo, Yeun Su ^a   Zhang, Zhuohua ^a  

^a BURNHAM INSTITUTE   (United States)

Author keywords

Biochemistry; Cell Biology; Cultured cell; Immunoblotting; Immunoprecipitation; In vitro system; Issue 30; Posttranslational modification; Ubiquitin; Ubiquitination

Indexed keywords

EID: 80055111957     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1293     Document Type: Article

References (4)

1. Xiong, H. et al. Pakrin, PINK1, and DJ-1 form a ubiquitin E3 ligase complex promoting unfolded protein degradation. J Clin Invest 119, 650-660 (2009).
2. Xirodimas, D. P., Saville, M. K., Bourdon, J. C., Hay, R. T. & Lane, D. P. Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity. Cell 118, 83-97 (2004).
3. Didier, C. et al. RNF5, a RING finger protein that regulates cell motility by targeting paxillin ubiquitination and altered localization. Mol Cell Biol 23, 5331-45 (2003).
4. Laney, J. D. & Hochsetrasser, M. Unit 14.5 Analysis of Protein Ubiquitination. Current Protocols in Protein Science, 14.5.1-14.5.11 (2002).