Primer extension capture: Targeted sequence retrieval from heavily degraded DNA sources

Journal of Visualized Experiments

Volumn , Issue 31, 2009, Pages

  Briggs, Adrian W ^a   Good, Jeffrey M ^a   Green, Richard E ^a   Krause, Johannes ^a   Maricic, Tomislav ^a   Stenzel, Udo ^a   Pääbo, Svante ^a  

^a MAX PLANCK INSTITUTE FOR EVOLUTIONARY ANTHROPOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055111869     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1573     Document Type: Article

References (5)

1. Briggs AW, Good JM, Green RE, Krause J, Maricic T, Stenzel U, Lalueza-Fox C, Rudan P, Brajkovic D, Kucan Z, Gusic I, Schmitz R, Doronichev VB, Golovanova LV, de la Rasilla M, Fortea J, Rosas A, Paabo S. Targeted retrieval and analysis of five Neandertal mtDNA genomes. Science 325(5938): 318-321 (2009).
2. Rohland N, Hofreiter M. Comparison and optimization of ancient DNA extraction. Biotechniques 42(3): 343-352 (2007).
3. Rohland N, Hofreiter M. Ancient DNA extraction from bones and teeth. Nat Protoc 2(7): 1756-1762 (2007).
4. Maricic T, Pääbo S. Optimization of 454 sequencing library preparation from small amounts of DNA permits sequence determination of both DNA strands Biotechniques 46(1): 51-57 (2009).
5. Meyer M, Briggs AW, Maricic T, Hober B, Hoffner B, Krause J, Weihmann A, Paabo S, Hofreiter M. From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing. Nucleic Acids Res 36 (1) e5 (2007).