MALDI sample preparation: The ultra thin layer method

Journal of Visualized Experiments

Volumn , Issue 3, 2007, Pages

  Fenyo, David ^a   Wang, Qingjun ^a   DeGrasse, Jeffrey A ^a   Padovan, Julio C ^a   Cadene, Martine ^a   Chait, Brian T ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Cellular biology; Issue 3; MALDI; Mass spectrometry; MS; Proteins; Ultra thin layer

Indexed keywords

EID: 80055109798     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/192     Document Type: Article

References (3)

1. F. Xiang, R.C. Beavis, A Method to Increase Contaminant Tolerance in Protein Matrix-Assisted Laser Desorption Ionization by the Fabrication of Thin Protein-Doped Polycrystalline Films, Rapid Communications in Mass Spectrometry 8, 199-204 (1994).
2. M. Cadene, B.T. Chait, A Robust, Detergent-Friendly Method for Mass Spectrometric Analysis of Integral Membrane Proteins, Analytical Chemistry 72, 5655-5658 (2000).
3. P. Hook, A. Mikami, B. Shafer, B.T. Chait, S.S. Rosenfeld, R.B. Vallee, Long-range allosteric control of cytoplasmic dynein ATPase activity by the stalk and C-terminal domains, J Biol Chem, 280, 33045-54, (2005).