Visualizing cell-to-cell transfer of HIV using fluorescent clones of HIV and live confocal microscopy

Journal of Visualized Experiments

Volumn , Issue 44, 2010, Pages

  Dale, Benjamin ^a   McNerney, Gregory P ^b   Thompson, Deanna L ^b   Hübner, Wolfgang ^c   Huser, Thomas ^b   Chen, Benjamin K ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055108956     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2061     Document Type: Article

References (5)

1. Blanco, J. et al. High level of coreceptor-independent HIV transfer induced by contacts between primary CD4 T cells. J Biol Chem 279, 51305-51314 (2004).
2. Sourisseau, M., Sol-Foulon, N., Porrot, F., Blanchet, F. & Schwartz, O. Inefficient human immunodeficiency virus replication in mobile lymphocytes. J Virol 81, 1000-1012 (2007).
3. Jolly, C., Kashefi, K., Hollinshead, M. & Sattentau, Q. J. HIV-1 cell to cell transfer across an Env-induced, actin-dependent synapse. J Exp Med 199, 283-293, (2004).
4. Hubner, W. et al. Sequence of human immunodeficiency virus type 1 (HIV-1) Gag localization and oligomerization monitored with live confocal imaging of a replication-competent, fluorescently tagged HIV-1. J Virol 81, 12596-12607 (2007).
5. Hubner, W. et al. Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science 323, 1743-1747 (2009).