Non-radioactive in situ hybridization protocol applicable for Norway spruce and a range of plant species

Journal of Visualized Experiments

Volumn , Issue 26, 2009, Pages

  Karlgren, Anna ^a   Carlsson, Jenny ^b   Gyllenstrand, Niclas ^b   Lagercrantz, Ulf ^a   Sundström, Jens F ^b  

^a UPPSALA UNIVERSITY   (Sweden)

^b SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCES   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055108832     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1205     Document Type: Article

References (17)

1. Carr, S.M. & Irish, V.F., Floral homeotic gene expression defines developmental arrest stages in Brassica oleracea L. vars. botrytis and italica. Planta 201 (2), 179-188 (1997).
2. Coen, E.S. & Meyerowitz, E.M., The war of the whorls: genetic interactions controlling flower development. Nature 353 (6339), 31-37 (1991).
3. Jackson, D., In situ hybridization in plants. in Molecular Plant Patology: a practical approach, edited by S.J. Gurr, M.J. McPherson, & D.J. Bowles (Oxford University Press, Oxford, 1991).
4. Leitch, A.R., Schwarzacher, T., Jackson, D., & Leitch, I.J., In situ hybridization: a practical guide. (BIOS Scientific Publishers Limited, Oxford, 1994).
5. Smyth, D.R., Bowman, J.L., & Meyerowitz, E.M., Early flower development in Arabidopsis. Plant Cell 2 (8), 755-767 (1990).
6. Azevedo, H., Lino-Neto, T., & Tavares, R.M., An improved method for high-quality RNA isolation from needles of adult maritime pine trees Plant Molecular Biology Reporter 21, 333-338 (2003).
7. Sundstrom, J. et al., MADS-box genes active in developing pollen cones of Norway spruce (Picea abies) are homologous to the B-class floral homeotic genes in angiosperms. Dev Genet 25 (3), 253-266 (1999).
8. Tandre, K., Svenson, M., Svensson, M.E., & Engstrom, P., Conservation of gene structure and activity in the regulation of reproductive organ development of conifers and angiosperms. Plant J 15 (5), 615-623 (1998).
9. Winter, K.U. et al., MADS-box genes reveal that gnetophytes are more closely related to conifers than to flowering plants. Proc Natl Acad Sci U S A 96 (13), 7342-7347 (1999).
10. Savard, L. et al., Chloroplast and nuclear gene sequences indicate late Pennsylvanian time for the last common ancestor of extant seed plants. Proc Natl Acad Sci U S A 91 (11), 5163-5167 (1994).
11. Jack, T., Brockman, L.L., & Meyerowitz, E.M., The homeotic gene APETALA3 of Arabidopsis thaliana encodes a MADS box and is expressed in petals and stamens. Cell 68 (4), 683-697 (1992).
12. Bowman, J.L., Smyth, D.R., & Meyerowitz, E.M., Genes directing flower development in Arabidopsis. Plant Cell 1 (1), 37-52 (1989).
13. Kramer, E.M. & Irish, V.F., Evolution of genetic mechanisms controlling petal development. Nature 399 (6732), 144-148 (1999).
14. Sundstrom, J. & Engstrom, P., Conifer reproductive development involves B-type MADS-box genes with distinct and different activities in male organ primordia. Plant J 31 (2), 161-169 (2002).
15. Carlsson, J. et al., Microarray analysis reveals altered expression of a large number of nuclear genes in developing cytoplasmic male sterile Brassica napus flowers. Plant J 49 (3), 452-462 (2007).
16. Teixeira, R.T., Farbos, I., & Glimelius, K., Expression levels of meristem identity and homeotic genes are modified by nuclear-mitochondrial interactions in alloplasmic male-sterile lines of Brassica napus. Plant J 42 (5), 731-742 (2005).
17. Ying, S. & Kay, A.B., The technique of in situ hybridization. Methods in Molecular Medicine 56, 263-283 (2001).