An in vitro skin irritation test (SIT) using the EpiDerm reconstructed human epidermal (RHE) model

Journal of Visualized Experiments

Volumn , Issue 29, 2009, Pages

  Kandárová, Helena ^a   Hayden, Patrick ^a   Klausner, Mitchell ^a   Kubilus, Joseph ^a   Sheasgreen, John ^a  

^a MATTEK CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055107543     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1366     Document Type: Article

References (9)

1. Kandárová H., Liebsch M., Genschow E., Ingrid Gerner, Traue D., Slawik B., Spielmann H.: Optimization of the EpiDerm Test Protocol for the upcoming ECVAM Validation Study on In Vitro Skin Irritation Tests. ALTEX, 21, 3/04, 107-114 (2004).
2. Kandárová, H., Liebsch, M., Gerner, I., Schmidt, E., Genschow, E., Traue, D., Spielmann, H. EpiDerm Skin Irritation Test Protocol Assessment of the performance of the optimized test. ATLA, 33 (4), 351-367. (2005).
3. Kandárová H., Hayden P., Klausner M., Kubilus J., Kearney P., Sheasgreen J. In Vitro Skin Irritation Test: Improving the Sensitivity of the EpiDerm Skin Irritation Test Protocol. Accepted for publication in ATLA (2009). Abstract In: Toxicology Letters, Abstracts of the 44th Congress of the European Societies of Toxicology Volume 172, Supplement 1-7, Page 81 (2007).
4. Spielmann, H., Hoffmann, S., Liebsch, M., Botham, P., Fentem, J., Eskes, C., Roguet, R., Cotovi, J., Cole, T., Worth, A., Heylings, J., Jones, P., Robles, C., Kandárová, H., Gamer, A., Remmele, M., Curren, R., Raabe, H., Cockshott, A., Gerner, I. and Zuang, V. The ECVAM International Validation Study on In Vitro Tests for Acute Skin Irritation: Report on the Validity of the EPISKIN and EpiDerm Assays and on the Skin Integrity Function Test. ATLA, 35, 559-601 (2007).
5. Liebsch M., Gamer A., Curren R., Frank J., Genschow E., Tharmann J., Remmele M., Bauer B., Raabe H., Barnes N., Hilberer A., Wilt N., Lornejad-Schäfer MR., Schäfer C., Spiller E., Hayden P., Kandárová H.: Follow-up validation of the modified EpiDerm Skin Irritation Test (SIT): results of a multicentre study of twenty reference test substances. 15th Congress on Alternatives to Animal Experimentation, Septemner 19-21.Linz, Austria. In: ALTEX, Volume 25, Supl 1 Linz/2008, ISSN 0946-7785 1-100, 43. (2008) (manuscript in preparation).
6. United Nations (UN) (2007). Globally Harmonized System of Classification and Labeling of Chemicals (GHS), Second revised edition, UN New York and Geneva, (2007). Available: [http://www.unece.org/trans/danger/publi/ghs/ghs_rev02/02files_e.html]
7. ESAC 2008: Statement on the Scientific Validity of In-Vitro Tests for Skin Irritation Testing, November 2008. Downloadable from http://ecvam.jrc.it/.
8. Gupta K., Rispin A., Stitzel K., Coecke S, Harbell J.: Ensuring quality of in vitro alternative test methods: Issues and answers. Regul Toxicol Pharmacol. Dec; 43(3):219-24 (2005).
9. Rispin, A., Stitzel, K., Harbell, J., Klausner, M.: Ensuring quality of in vitro alternative test methods: Current practice. Regul Toxicol Pharmacol. Jul;45(2):97-103 (2006).