Chip-based three-dimensional cell culture in perfused micro-bioreactors

Journal of Visualized Experiments

Volumn , Issue 15, 2008, Pages

  Gottwald, Eric ^a   Lahni, Brigitte ^a   Thiele, David ^a   Giselbrecht, Stefan ^a   Welle, Alexander ^a   Weibezahn, Karl Friedrich ^a  

^a Forschungszentrum Karlsruh   (Germany)

Author keywords

Bioreactor; Cellular biology; Chip; Issue 15; Perfusion; Three dimensional

Indexed keywords

EID: 80055105492     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/564     Document Type: Article

References (6)

1. Berry, M.N., Friend, D.S. High-yield preparation of isolated rat liver parenchymal cells: A biochemical and fine structural study. J. Cell Biol. 53, 506-520 (1969)
2. Seglen, P.O. Preparation of rat liver cells. 3. Enzymatic requirements for tissue dispersion. Exp. Cell Res. 82, 391-398 (1973)
3. Giselbrecht, S., Gietzelt, T., Gottwald, E., Trautmann, C., Truckenmüller, R., Weibezahn, K.F., Welle, A. 3D tissue culture substrates produced by microthermoforming of pre-processed polymer films. Biomed. Microdev. 8, 191-199 (2006)
4. Welle, A., Gottwald, E. UV-based patterning of polymeric substrates for cell culture applications. Biomed. Microdev. 4 (1), 33-41 (2002)
5. Gottwald, E., Giselbrecht, S., Augspurger, C., Lahni, B., Dambrowsky, N., Truckenmüller, R., Piotter, V., Gietzelt, T., Wendt, O., Pfleging, W., Welle, A., Rolletschek, A., Wobus, A.M., Weibezahn, K.-F. A chip-based platform for the in vitro generation of tissues in three-dimensional organization. Lab Chip 7(6), 777-785 (2007)
6. Eschbach, E., Chatterjee, S.S., Nöldner, M., Gottwald, E., Dertinger, H., Weibezahn, K.-F., Knedlitschek, G. Microstructured scaffolds for liver tissue with high density: Morphological and biochemical characterization of tissue aggregates. J. Cell. Biochem. 95, 243-255 (2005)