Assaying locomotor activity to study circadian rhythms and sleep parameters in Drosophila

Journal of Visualized Experiments

Volumn , Issue 43, 2010, Pages

  Chiu, Joanna C ^a,b   Low, Kwang Huei ^a,c   Pike, Douglas H ^a   Yildirim, Evrim ^a,c   Edery, Isaac ^a,c  

^a RUTGERS UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c The State University of New Jersey   (United States)

Author keywords

Circadian rhythm; Drosophila; Issue 43; Locomotor activity; Neuroscience; Period; Sleep; Trikinetics

Indexed keywords

EID: 80055103245     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2157     Document Type: Article

References (16)

1. Brand, A. H. & Perrimon, N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118, 401-415 (1993).
2. Chen, W. F., Low, K. H., Lim, C., & Edery, I. Thermosensitive splicing of a clock gene and seasonal adaptation. Cold Spring Harb Symp Quant Biol 72, 599-606 (2007).
3. Chiu, J. C., Vanselow, J. T., Kramer, A., & Edery, I. The phospho-occupancy of an atypical SLIMB-binding site on PERIOD that is phosphorylated by DOUBLETIME controls the pace of the clock. Genes Dev 22, 1758-1772 (2008).
4. Dubruille, R., Murad, A., Rosbash, M., & Emery, P. A constant light-genetic screen identifies KISMET as a regulator of circadian photoresponses. PLoS Genet 12, e1000787 (2009).
5. Glaser, F. T. & Stanewsky, R. Temperature synchronization of the Drosophila circadian clock. Curr Biol 15, 1352-1363 (2005).
6. Helfrich-Förster, C. Differential control of morning and evening components in the activity rhythm of Drosophila melanogaster-sex-specific differences suggest a different quality of activity. J Biol Rhythms 15, 135-154 (2000).
7. Hendricks, J. C., Finn, S. M., Pancleri, K. A., Chavkin, J., Williams, J., Sehgal, A., & Pack, A. I. Rest in Drosophila is a sleep-like state. Neuron 25, 129:138 (2000).
8. Ho, K. S. & Sehgal, A. Drosophila melanogaster: an insect model for fundamental studies of sleep. Methods Enzymol 393, 772-793 (2005).
9. Koh, K., Evans, J. M., Hendricks, J. C., & Sehgal, A. A Drosophila model for age-associated changes in sleep:wake cycles. Proc Natl Acad Sci USA 103, 13843-13847 (2006).
10. Konopka, R. J., & Benzer, S. Clock mutants of Drosophila melanogaster. Proc Natl Acad Sci USA 68, 2112-2116 (1971).
11. McGuire, S. E., Roman, G., & Davis, R. L. Gene expression systems in Drosophila: a synthesis of time and space. Trends Genet 20, 384-391 (2004).
12. Osterwalder, T., Yoon, K. S., White, B. H., & Keshishian, H. A conditional tissue-specific transgene expression system using inducible GAL4. Proc Natl Acad Sci USA 98, 12596-12601 (2001).
13. Rosato, E. & Kyriacou, C. P. Analysis of locomotor activity rhythms in Drosophila. Nature Protocols 1, 559-568 (2006).
14. Sehadova, H., Glaser, F. T., Gentile, C., Simoni, A., Giesecke, A., Albert, J. T., & Stanewsky, R. Temperature entrainment of Drosophila's circadian clock involves the gene nocte and signaling from peripheral sensory tissues to the brain. Neuron 64, 251-266 (2009).
15. Zimmerman, J. E., Raizen, D. M., Maycock, M. H., Maislin, G., & Pack, A. I. A video method to study Drosophila sleep. Sleep 31, 1587-1598 (2008).
16. Zordan, M. A., Benna, C., & Mazzotta, G. Monitoring and analyzing Drosophila circadian locomotor activity. In Circadian Rhythms Methods and Protocols (ed. Rosato, E.) Methods in Molecular Biology series (Humana, Totowa, New Jersey, 2007).