Antibody profiling by Luciferase Immunoprecipitation Systems (LIPS)

Journal of Visualized Experiments

Volumn , Issue 32, 2009, Pages

  Burbelo, Peter D ^a   Ching, Kathryn H ^a   Klimavicz, Caitlin M ^a   Iadarola, Michael J ^a  

^a NATIONAL INSTITUTE OF DENTAL AND CRANIOFACIAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055102756     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/1549     Document Type: Article

References (10)

1. Burbelo, P.D., Goldman, R. & Mattson, T.L. A simplified immunoprecipitation method for quantitatively measuring antibody responses in clinical sera samples by using mammalian-produced Renilla luciferase-antigen fusion proteins. BMC Biotechnol 5, 22 (2005).
2. Burbelo, P.D., Leahy, H.P., Iadarola, M.J. & Nutman, T.B. A Four-Antigen Mixture for Rapid Assessment of Onchocerca volvulus Infection. PLoS Negl Trop Dis 3, e438 (2009).
3. Burbelo, P.D. Sensitive and robust luminescent profiling of anti-La and other autoantibodies in Sj gren's syndrome Autoimmunity 139, 241-5 (2009).
4. Burbelo, P.D. et al. Rapid antibody quantification and generation of whole proteome antibody response profiles using LIPS (luciferase immunoprecipitation systems). Biochem Biophys Res Commun 352, 889-95 (2007).
5. Burbelo, P.D., Groot, S., Dalakas, M.C. & Iadarola, M.J. High definition profiling of autoantibodies to glutamic acid decarboxylases GAD65/GAD67 in stiff-person syndrome. Biochem Biophys Res Commun 366, 1-7 (2008).
6. Burbelo, P.D. et al. A new luminescence assay for autoantibodies to mammalian cell-prepared insulinoma-associated protein 2. Diabetes Care 31, 1824-6 (2008).
7. Burbelo, P.D. et al. Serological diagnosis of human herpes simplex virus type 1 and 2 infections by luciferase immunoprecipitation system assay. Clin Vaccine Immunol 16, 366-71 (2009).
8. Burbelo, P.D. et al. Highly quantitative serological detection of anti-cytomegalovirus (CMV) antibodies. Virol J 6, 45 (2009).
9. Burbelo, P.D. et al. Four-antigen mixture containing v-cyclin for serological screening of human herpesvirus 8 infection. Clin Vaccine Immunol 16, 621-7 (2009).
10. Ramanathan, R. et al. A luciferase immunoprecipitation systems assay enhances the sensitivity and specificity of diagnosis of Strongyloides stercoralis infection. J Infect Dis 198, 444-51 (2008).