Quantifying synapses: An immunocytochemistry-based assay to quantify synapse number

Journal of Visualized Experiments

Volumn , Issue 45, 2010, Pages

  Ippolito, Dominic M ^a   Eroglu, Cagla ^a  

^a DUKE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Astrocyte; Brain; Immunocytochemistry; Issue 45; Neuron; Neuroscience; Synapse

Indexed keywords

EID: 80055102539     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2270     Document Type: Article

References (11)

1. Barres, B.A., Silverstein, B.E., Corey, D.P. & Chun, L.L.Y. Immunological, morphological, and electrophysiological variation among retinal ganglion cells purified by panning. Neuron 1, 791-803 (1988).
2. Meyer-Franke, A., Kaplan, M.R., Pfrieger, F.W. & Barres, B.A. Characterization of the signaling interactions that promote the survival and growth of developing retinal ganglion cells in culture. Neuron 15, 805-819 (1995).
3. Eroglu, C. et al. Gabapentin receptor alpha2delta-1 is a neuronal thrombospondin receptor responsible for excitatory CNS synaptogenesis. Cell 139, 380-392 (2009).
4. Pfrieger, F.W. & Barres, B.A. Synaptic efficacy enhanced by glial cells in vitro. Science 277, 1684-1687.
5. Ullian, E.M., Sapperstein, S.K., Christopherson, K.S. & Barres, B.A. Control of synapse number by glia. Science 291, 657-661 (2001).
6. Christopherson, K.S. et al. Thrombospondins are astrocyte-secreted proteins that promote CNS synaptogenesis. Cell 120, 421-433 (2005).
7. Godement, P., Salaun, J. & Imbert, M. Prenatal and postnatal development of retinocollicular projections in the mouse. J. Comp. Neurol. 230, 552-575 (1985).
8. Schmidt, J.T. Formation of retinotopic connections: selective stabilization by an activity-dependent mechanism. Cell. Mol. Neurobiol. 5, 65-84 (1985).
9. Sachs, G.M., Jacobson, M. & Caviness, V.S., Jr. Postnatal changes in arborization patterns of murine retinocollicular axons. J. Comp. Neurol. 246, 395-408 (1986).
10. Elmariah, S.B., Oh, E.J., Hughes, E.G. & Balice-Gordon, R.J. Astrocytes regulate inhibitory synapse formation via Trk-mediated modulation of postsynaptic GABAA receptors. J. Neurosci. 25, 3638-3650 (2005).
11. Hughes, E.G., Elmariah, S.B. & Balice-Gordon, R.J. Astrocyte secreted proteins selectively increase hippocampal GABAergic length, branching and synaptogenesis. Moll. Cell. Neurosci. 43, 136-145 (2010).