Western blotting using the Invitrogen Nupage novex Bis tris MiniGels

Journal of Visualized Experiments

Volumn , Issue 7, 2007, Pages

  Penna, Aubin ^a   Cahalan, Michael ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80055101339     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/264     Document Type: Article

References (9)

1. Gel Migration chart: http://www.invitrogen.com/etc/medialib/en/filelibrary/pdf.Par.99253.Filedat/O-063575-NuPage_fin.pdf
2. Complete western-blot protocol from the Invitrogen website: https://commerce.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeId=A2C5758BC7C3438B01378A0940376C8E
3. Nu PAGE technical guide: http://tools.invitrogen.com/content/sfs/manuals/nupage_tech_man.pdf
4. Instruction Manual, Mini Trans-Blot Cell: http://www.bio-rad.com/cmc_upload/0/000/013/280/M1703930E.pdf
5. ECL Plus Western blotting detection reagents instruction: http://www5.gelifesciences.com/aptrix/upp00919.nsf/Content/139B41E53EDC6210C1256EB40044ACE3/$file/RPN2132PL_Rev_D_2006_web.pdf
6. Estimation of protein molecular weights by SDS gel electrophoresis, GE Healthcare Website: http://www1.gelifesciences.com/aptrix/upp00919.nsf/Content/elpho_applications~elpho_applications_1d_protein_analysis~elpho_sds_page~Elpho_1D_SDS+PAGE+2+Estimation+of+protein+molecular+weights+by+SDS+gel+electro phoresis+~3.+Procedure
7. Yeromin, A. V. et al. Molecular identification of the CRAC channel by altered ion selectivity in a mutant of Orai. Nature 443, 226-9 (2006).
8. Zhang, S. L. et al. Store-dependent and -independent modes regulating CRAC channel activity of human Orai1 and Orai3. J. Biol. Chem. 283, 17662-17671 (2008).
9. Penna, A., et al. The CRAC channel consists of a tetramer formed by Stim-induced dimerization of Orai dimers. Nature (in press)