A novel framework for automated targeting of unstained living cells in bright field microscopy

Proceedings - International Symposium on Biomedical Imaging

Volumn , Issue , 2011, Pages 195-198

  Becattini, Gabriele ^a   Mattos, Leonardo S ^a   Caldwell, Darwin G ^a  

^a ISTITUTO ITALIANO DI TECNOLOGIA   (Italy)

Author keywords

Cell segmentation; contour completion; defocusing microscopy; watershed

Indexed keywords

ADHERENT CELLS; AUTOMATIC LOCALIZATION; BRIGHT-FIELD MICROSCOPY; BRIGHTFIELD; CELL IDENTIFICATION; CELL SEGMENTATION; CONTOUR COMPLETION; CRITICAL STEPS; DEFOCUSING; INTEGRATED ALGORITHM; INTENSITY VARIATIONS; LIVING CELL; MICRO-INJECTION; PROCESSING APPROACH; REAL IMAGES; STATE OF THE ART; UNIFIED FRAMEWORK;

COMMINUTION; IMAGE PROCESSING; MATHEMATICAL OPERATORS; MEDICAL IMAGING;

CELLS;

EID: 80055060006     PISSN: 19457928     EISSN: 19458452     Source Type: Conference Proceeding    
DOI: 10.1109/ISBI.2011.5872386     Document Type: Conference Paper

References (8)

1. Y. Zhang, K. K. Tan and S. Huang, "Software Based Vision System for Automated Cell Injection" International Conference on Biomedical Engineering and Informatics, pp.718-722, 2008.
2. V. V. Lulevich, Y. Shih, S. H. Lo and G. Liu, "Cell Tracing Dyes Significantly Change Single Cell Mechanics," J. Phys. Chem. B, 113 (18), pp 6511-6519, 2009.
3. U. Agero, C. H. Monken, C. Ropert, "Cell surface fluctuations studied with defocusing microscopy," Phys. Rev. E 67, 2003.
4. K. Wu, D. Gauthier, M. Levine, "Live Cell Image Segmentation", IEEE Transactions on Biomedical Engineering, 42, 1-12, 1995.
5. M. Tscherepanow, F. Zöllner, M. Hillebrand and F. Kummert, "Automatic Segmentation of Unstained Living Cells in Bright-Field Microscope Images," Lecture Notes in Computer Science, 5108, pp. 158-172, 2008.
6. D. Gil, P. Radeva, "Extending anisotropic operators to recover smooth shapes," Computer Vision and Image Understanding, 99 110-125, 2005.
7. G. Becattini, L. S. Mattos, D. G. Caldwell, "Diffusion tensor driven contour closing for cell microinjection targeting," 32nd EMBC, pp.4072-4075, 2010.
8. J. C. Russ, "Segmentating Touching Hollow Features," J. Computer Assisted Microscopy, 7, pp.253-261, 1995.