The opportunistic pathogen Serratia marcescens utilizes type VI secretion to target bacterial competitors

Journal of Bacteriology

Volumn 193, Issue 21, 2011, Pages 6057-6069

  Murdoch, Sarah L ^a   Trunk, Katharina ^a   English, Grant ^a   Fritsch, Maximilian J ^a   Pourkarimi, Ehsan ^a   Coulthurst, Sarah J ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIAL GENE; BACTERIUM COMPETENCE; CULTURE MEDIUM; ENTEROBACTER CLOACAE; GRAM NEGATIVE BACTERIUM; NONHUMAN; PRIORITY JOURNAL; SERRATIA MARCESCENS; TYPE VI SECRETION SYSTEM;

ANTI-BACTERIAL AGENTS; ANTIBIOSIS; BACTERIAL PROTEINS; ENTEROBACTER CLOACAE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, BACTERIAL; MEMBRANE TRANSPORT PROTEINS; MICROBIAL VIABILITY; SERRATIA MARCESCENS;

BACTERIA (MICROORGANISMS); ENTEROBACTER CLOACAE; EUKARYOTA; NEGIBACTERIA; SERRATIA MARCESCENS;

EID: 80055042052     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.05671-11     Document Type: Article

References (41)

1. Altschul, S. F., et al. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Bernard, C. S., Y. R. Brunet, E. Gueguen, and E. Cascales. 2010. Nooks and crannies in type VI secretion regulation. J. Bacteriol. 192:3850-3860.
3. Bingle, L. E., C. M. Bailey, and M. J. Pallen. 2008. Type VI secretion: a beginner's guide. Curr. Opin. Microbiol. 11:3-8.
4. Blondel, C. J., et al. 2010. Contribution of the type VI secretion system encoded in SPI-19 to chicken colonization by Salmonella enterica serotypes Gallinarum and Enteritidis. PLoS One 5:e11724.
5. Bonemann, G., A. Pietrosiuk, and A. Mogk. 2010. Tubules and donuts: a type VI secretion story. Mol. Microbiol. 76:815-821.
6. Boyer, F., G. Fichant, J. Berthod, Y. Vandenbrouck, and I. Attree. 2009. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics 10:104.
7. Burtnick, M. N., et al. 2011. The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect. Immun. 79:1512-1525.
8. Casadaban, M. J., and S. N. Cohen. 1979. Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proc. Natl. Acad. Sci. U. S. A. 76:4530-4533.
9. Cascales, E. 2008. The type VI secretion toolkit. EMBO Rep. 9:735-741.
10. Choi, S. H., et al. 2007. Prevalence, microbiology, and clinical characteristics of extended-spectrum beta-lactamase-producing Enterobacter spp., Serratia marcescens, Citrobacter freundii, and Morganella morganii in Korea. Eur. J. Clin. Microbiol. Infect. Dis. 26:557-561.
11. Coulthurst, S. J., K. S. Lilley, and G. P. Salmond. 2006. Genetic and proteomic analysis of the role of luxS in the enteric phytopathogen, Erwinia carotovora. Mol. Plant Pathol. 7:31-45.
12. Coulthurst, S. J., N. R. Williamson, A. K. Harris, D. R. Spring, and G. P. Salmond. 2006. Metabolic and regulatory engineering of Serratia marcescens: mimicking phage-mediated horizontal acquisition of antibiotic biosynthesis and quorum-sensing capacities. Microbiology 152:1899-1911.
13. de Lorenzo, V., M. Herrero, U. Jakubzik, and K. N. Timmis. 1990. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J. Bacteriol. 172:6568-6572.
14. de Pace, F., et al. 2010. The type VI secretion system plays a role in type 1 fimbria expression and pathogenesis of an avian pathogenic Escherichia coli strain. Infect. Immun. 78:4990-4998.
15. Filloux, A., A. Hachani, and S. Bleves. 2008. The bacterial type VI secretion machine: yet another player for protein transport across membranes. Microbiology 154:1570-1583.
16. Flyg, C., K. Kenne, and H. G. Boman. 1980. Insect pathogenic properties of Serratia marcescens: phage-resistant mutants with a decreased resistance to Cecropia immunity and a decreased virulence to Drosophila. J. Gen. Microbiol. 120:173-181.
17. Gerlach, R. G., and M. Hensel. 2007. Protein secretion systems and adhesins: the molecular armory of Gram-negative pathogens. Int. J. Med. Microbiol. 297:401-415.
18. Grinter, N. J. 1983. A broad-host-range cloning vector transposable to various replicons. Gene 21:133-143.
19. Hejazi, A., and F. R. Falkiner. 1997. Serratia marcescens. J. Med. Microbiol. 46:903-912.
20. Herrero, M., V. de Lorenzo, and K. N. Timmis. 1990. Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J. Bacteriol. 172:6557-6567.
21. Holland, I. B. 2010. The extraordinary diversity of bacterial protein secretion mechanisms. Methods Mol. Biol. 619:1-20.
22. Hood, R. D., et al. 2010. A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe 7:25-37.
23. Hsu, F., S. Schwarz, and J. D. Mougous. 2009. TagR promotes PpkAcatalysed type VI secretion activation in Pseudomonas aeruginosa. Mol. Microbiol. 72:1111-1125.
24. Jack, R. L., et al. 2004. Coordinating assembly and export of complex bacterial proteins. EMBO J. 23:3962-3972.
25. Jani, A. J., and P. A. Cotter. 2010. Type VI secretion: not just for pathogenesis anymore. Cell Host Microbe 8:2-6.
26. Kaniga, K., I. Delor, and G. R. Cornelis. 1991. A wide-host-range suicide vector for improving reverse genetics in gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica. Gene 109:137-141.
27. Kunz, A. N., and I. Brook. 2010. Emerging resistant Gram-negative aerobic bacilli in hospital-acquired infections. Chemotherapy 56:492-500.
28. Kurz, C. L., et al. 2003. Virulence factors of the human opportunistic pathogen Serratia marcescens identified by in vivo screening. EMBO J. 22:1451-1460.
29. Leiman, P. G., et al. 2009. Type VI secretion apparatus and phage tailassociated protein complexes share a common evolutionary origin. Proc. Natl. Acad. Sci. U. S. A. 106:4154-4159.
30. Liu, H., et al. 2008. Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum. PLoS Pathog. 4:e1000093.
31. Lockhart, S. R., et al. 2007. Antimicrobial resistance among gram-negative bacilli causing infections in intensive care unit patients in the United States between 1993 and 2004. J. Clin. Microbiol. 45:3352-3359.
32. MacIntyre, D. L., S. T. Miyata, M. Kitaoka, and S. Pukatzki. 2010. The Vibrio cholerae type VI secretion system displays antimicrobial properties. Proc. Natl. Acad. Sci. U. S. A. 107:19520-19524.
33. Miyata, S., M. Casey, D. W. Frank, F. M. Ausubel, and E. Drenkard. 2003. Use of the Galleria mellonella caterpillar as a model host to study the role of the type III secretion system in Pseudomonas aeruginosa pathogenesis. Infect. Immun. 71:2404-2413.
34. Moriel, D. G., et al. 2010. Identification of protective and broadly conserved vaccine antigens from the genome of extraintestinal pathogenic Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 107:9072-9077.
35. Mougous, J. D., C. A. Gifford, T. L. Ramsdell, and J. J. Mekalanos. 2007. Threonine phosphorylation post-translationally regulates protein secretion in Pseudomonas aeruginosa. Nat. Cell Biol. 9:797-803.
36. Pukatzki, S., A. T. Ma, A. T. Revel, D. Sturtevant, and J. J. Mekalanos. 2007. Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin. Proc. Natl. Acad. Sci. U. S. A. 104:15508-15513.
37. Pukatzki, S., S. B. McAuley, and S. T. Miyata. 2009. The type VI secretion system: translocation of effectors and effector-domains. Curr. Opin. Microbiol. 12:11-17.
38. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
39. Schwarz, S., et al. 2010. Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathog. 6:e1001068.
40. Shalom, G., J. G. Shaw, and M. S. Thomas. 2007. In vivo expression technology identifies a type VI secretion system locus in Burkholderia pseudomallei that is induced upon invasion of macrophages. Microbiology 153:2689-2699.
41. Zheng, J., and K. Y. Leung. 2007. Dissection of a type VI secretion system in Edwardsiella tarda. Mol. Microbiol. 66:1192-1206.