Determination of low bacterial concentrations in hyperarid Atacama soils: Comparison of biochemical and microscopy methods with realtime quantitative PCR

Canadian Journal of Microbiology

Volumn 57, Issue 11, 2011, Pages 953-963

  Fletcher, Lauren E ^a,b   Conley, Catharine A ^c   Valdivia Silva, Julio E ^b   Perez Montaño, Saul ^b,d   Condori Apaza, Renee ^e   Kovacs, Gregory T A ^f   Glavin, Daniel P ^g   McKay, Christopher P ^b  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b NASA AMES RESEARCH CENTER   (United States)

^c NASA HEADQUARTERS   (United States)

^d SAN JOSE STATE UNIVERSITY   (United States)

^e UNIVERSIDAD NACIONAL DE SAN AGUSTÍN DE AREQUIPA   (Peru)

^f Stanford University   (United States)

^g NASA GODDARD SPACE FLIGHT CENTER   (United States)

Author keywords

Atacama Desert; Hyperarid environment; Real time qPCR; Soil DNA

Indexed keywords

ATACAMA; ATACAMA DESERT; BACTERIA CONCENTRATIONS; DNA CONCENTRATION; DNA EXTRACTION; HYPERARID ENVIRONMENT; MICROBIAL CONCENTRATIONS; MICROBIAL POPULATIONS; MOLECULAR METHODS; ORDERS OF MAGNITUDE; PHOSPHOLIPID FATTY ACIDS; PHOSPHOLIPIDS FATTY ACIDS; QUANTITATIVE POLYMERASE CHAIN REACTION; REAL-TIME QPCR; REAL-TIME QUANTITATIVE PCR; SOIL BACTERIUM; SOIL DNA; STANDARD METHOD;

BACTERIA; BACTERIOLOGY; DNA; FATTY ACIDS; LANDFORMS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PHOSPHOLIPIDS; POLYMERIZATION; SOILS;

POLYMERASE CHAIN REACTION;

ADENINE; FATTY ACID;

ARID ENVIRONMENT; BIOCHEMICAL COMPOSITION; COMPARATIVE STUDY; FATTY ACID; MICROBIAL COMMUNITY; MICROSCOPY; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME; SOIL MICROORGANISM; SPECIES DIVERSITY;

ARTICLE; BACTERIAL LOAD; BACTERIAL STRAIN; BACTERIUM DETECTION; BIOCHEMISTRY; CLIMATE; CONTROLLED STUDY; DESERT; DESERT CLIMATE; DNA EXTRACTION; FATTY ACID ANALYSIS; LIQUID CHROMATOGRAPHY; MICROBIAL DIVERSITY; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SOIL ANALYSIS; TIME OF FLIGHT MASS SPECTROMETRY;

BACTERIA; BACTERIAL LOAD; BACTERIAL PHYSIOLOGICAL PHENOMENA; BIOCHEMISTRY; BIOMASS; DESERT CLIMATE; ENVIRONMENTAL MONITORING; FATTY ACIDS; MICROSCOPY; PHOSPHOLIPIDS; REAL-TIME POLYMERASE CHAIN REACTION; SOIL; SOIL MICROBIOLOGY;

ATACAMA DESERT; CHILE;

BACTERIA (MICROORGANISMS);

EID: 80055041058     PISSN: 00084166     EISSN: 14803275     Source Type: Journal    
DOI: 10.1139/w11-091     Document Type: Article

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