Detection of phytoplasmas in mixed infection with begomoviruses: A case study of tomato and pepper in Mexico

Bulletin of Insectology

Volumn 64, Issue SUPPL. 1, 2011, Pages

  Lebsky, Vladimir ^a   Hernández González, Julio ^a   Arguello Astorga, Gerardo ^b   Cardenas Conejo, Yair ^b   Poghosyan, Arevik ^a  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS DEL NOROESTE   (Mexico)

^b INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA   (Mexico)

Author keywords

BCS; Begomoviruses; Nested PCR; Pepper; Phytoplasma; RFLP; SEM; Tomato

Indexed keywords

BEGOMOVIRUS; CANDIDATUS PHYTOPLASMA; LYCOPERSICON ESCULENTUM;

EID: 80054852157     PISSN: 17218861     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. AROCHA Y., PIÑOL B., ACOSTA K., ALMEIDA R., DEVONSHIRE J., VAN DE MEENE A., BOA E., LUCAS J., 2009. Detection of phytoplasma and potivirus pathogens in papaya (Carica papaya L.) affected with "Bunchy top symptom" (BTS) in eastern Cuba. Crop Protection, 28(8): 640-646.
2. Cardenas-Conejo Y., Arguello-Astorga G., POGHOSYAN A., Hernandez-Gonzalez J., LEBSKY V., Holguin-Peña J., Medina-Hernandez D., Vega-Peña S., 2010. First report of Tomato yellow leaf curl virus co-infecting pepper with Tomato chino La Paz virus in Baja California Sur, Mexico. Plant Disease, 94(10): 1266.
3. GUNDERSEN D. E., Lee I-M., 1996. Ultrasensitive detection of phytoplasmas by nested PCR assays using two universal primers. Phytopatologia Mediterranea, 35: 144-151.
4. LEBSKY V., POGHOSYAN A., 2007. Phytoplasma associated diseases in tomato and pepper in the state of BCS, Mexico: a breaf overview. Bulletin of Insectology, 60(2): 131-132.
5. LEE I-M., Gundersen-Rindal D., DAVIS R. E., BARTOSZYK I., 1998. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. International Journal of Systematic Bacteriology, 48: 1153-1169.
6. Mauricio-Castillo J. A., Arguello-Astorga G. R., Ambriz-Granados S., Alpuche-Solis A.G., 2007. First report of Tomato golden mottle virus on Licopersicon esculentum and Solanum rostratum in Mexico. Plant Disease, 99(11): 1513.
7. POGHOSYAN A., LEBSKY V., Servin-Villegas R., LANDA L., 2008. Phytoplasma associated diseases in organic vegetable crops in Santo Domingo Valley. Phytopathology, 98(Supplement): S200.
8. WYATT S. D., BROWN J. K., 1996. Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology, 86: 1288-1293.
9. ZHANG Y. P., UYEMOTO J. P., KIRKPATRICK B. C., 1998. A small scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. Journal of Virology Methods, 71: 45-50.