Preparative-scale isoelectric trapping by recursive electrophoresis in a compartmentalized system having orthogonal primary and secondary pH gradients. Part 1 - Construction and standard operation

Electrophoresis

Volumn 32, Issue 20, 2011, Pages 2797-2804

  North, Robert Y ^a   Vigh, Gyula ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Buffering membrane; Isoelectric trapping; Multi compartmental electrolyzer; Preparative electrophoresis; T RECS; Trapping by recursive electrophoresis in a compartmentalized system

Indexed keywords

BUFFERING MEMBRANE; COMPARTMENTALIZED SYSTEMS; ELECTROLYZERS; ISOELECTRIC; ISOELECTRIC TRAPPING; MULTI-COMPARTMENTAL ELECTROLYZE; PREPARATIVE ELECTROPHORESIS; TRAPPING BY RECURSIVE ELECTROPHORESIS IN A COMPARTMENTALIZED SYSTEM;

ELECTROPHORESIS;

AMPHOLYTE;

ANALYTIC METHOD; ARTICLE; CONCENTRATION RESPONSE; ELECTRODE; FLOW MEASUREMENT; ION TRANSPORT; PH; PREPARATIVE SCALE ISOELECTRIC TRAPPING; SEPARATION TECHNIQUE; TRAPPING BY RECURSIVE ELECTROPHORESIS IN A COMPARTMENTALIZED SYSTEM;

EID: 80054845682     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.201100119     Document Type: Article

References (30)

1. Martin, A. J. P., Hampson, F., J. Chromatogr. 1978, 159, 101-110.
2. Martin, A. J. P., Hampson, F., US Patent 4 243 507, 1981.
3. Faupel, M., Barzaghi, B., Gelfi, C., Righetti, P. G., J. Biochem. Biophys. Methods 1987, 15, 147-162.
4. Faupel, M., Righetti, P. G., US Patent 4 971 670, 1990.
5. Righetti, P. G., Bossi, A., Wenisch, E., Orsini, G., J. Chromatogr. B 1997, 699, 105-115.
6. Cretich, M., Pirri, G., Carrea, G., Chiari, M., Electrophoresis 2003, 24, 577-581.
7. Lalwani, S., Shave, E., Fleisher, H. C., Nzeadibe, K., Busby, M. B., Vigh, G., Electrophoresis 2004, 25, 2128-2138.
8. Lalwani, S., Shave, E., Vigh, G., Electrophoresis 2004, 25, 3323-3330.
9. Fleisher, H. C., Vigh, G., Electrophoresis 2005, 26, 2511-2519.
10. Fleisher-Craver, H. C., Vigh, G., Electrophoresis 2008, 29, 4247-4256.
11. Righetti, P. G., Wenisch, E., Faupel, M., J. Chromatogr. 1989, 475, 293-309.
12. Zuo, X., Speicher, D. W., Anal. Biochem. 2000, 284, 266-278.
13. Lim, P. J., North, R. Y., Vigh, G., Electrophoresis 2007, 28, 1851-1859.
14. 2 Instruction Manual, Proteome Systems Ltd., Woburn, MA 2003.
15. Shang, T. Q., Ginter, J. M., Johnston, M. V., Larsen, B. S., McEwen, C. N., Electrophoresis 2003, 24, 2359-2368.
16. Ros, A., Faupel, M., Mees, H., van Oostrum, J., Righetti, P. G., Proteomics 2002, 2, 151-156.
17. Arnaud, I. L., Josserand, J., Rossier, J. S., Girault, H. H., Electrophoresis 2002, 23, 3253-3261.
18. Michel, P. E., Reymond, P., Arnaud, I. L., Josserand, J., Girault, H. H., Rossier, J. S., Electrophoresis 2003, 24, 3-11.
19. Ogle, D., Sheehan, M., Rumbel, B., Gibson, T., Shave, E., Lim, P., Vigh, G., J. Chromatogr. A 2003, 989, 65-72.
20. Shave, E., Vigh, G., Electrophoresis 2004, 25, 381-387.
21. Shave, E., Vigh, G., Electrophoresis 2007, 28, 2291-2299.
22. Zilberstein, G. V., Bakin, E. M., Bukshpan, S., Electrophoresis 2003, 24, 3735-3744.
23. Zilberstein, G. V., Bakin, E. M., Bukshpan, S., Korol, L. E., Electrophoresis 2004, 25, 3643-3651.
24. Anonymous, Digital Proteome Chip Fractionator Data Sheet, Cell Biosciences, Inc., Santa Clara, CA 2010.
25. Lim, P. J., Novel devices for analytical-scale isoelectric trapping separations, Dissertation, Texas A&M University, 2006.
26. Lim, P. J., Vigh, G., Electrophoresis 2011, 32, 1647-1653.
27. Lim, P. J., Vigh, G., Electrophoresis 2011, 32, 1654-1658.
28. Shave, E., Vigh, G., Electrophoresis 2007, 28, 587-594.
29. Shave, E., Vigh, G., J. Chromatogr. A 2003, 989, 73-78.
30. Shave, E., Vigh, G., J. Chromatogr. A 2004, 1036, 3-6.