Virulence of an exotoxin A-deficient strain of Pseudomonas aeruginosa toward the silkworm, Bombyx mori

Microbial Pathogenesis

Volumn 51, Issue 6, 2011, Pages 407-414

  Chieda, Yuuka ^a   Iiyama, Kazuhiro ^a   Lee, Jae Man ^a   Kusakabe, Takahiro ^a   Yasunaga Aoki, Chisa ^a   Shimizu, Susumu ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Bombyx mori; Exotoxin A; Pseudomonas aeruginosa; ToxA; Virulence

Indexed keywords

BACTERIAL TOXIN; EXOTOXIN A; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BACTERIUM MUTANT; COCOON; CONTROLLED STUDY; GENE MUTATION; GENETIC COMPLEMENTATION; HEMOLYMPH; INOCULATION; LARVAL STAGE; MICROBIAL COMMUNITY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SILKWORM; STRAIN DIFFERENCE; TOXA GENE;

ADP RIBOSE TRANSFERASES; ANIMALS; BACTERIAL LOAD; BACTERIAL TOXINS; BOMBYX; EXOTOXINS; GENE DELETION; GENETIC COMPLEMENTATION TEST; HEMOLYMPH; LARVA; PSEUDOMONAS AERUGINOSA; SURVIVAL ANALYSIS; VIRULENCE; VIRULENCE FACTORS;

BACTERIA (MICROORGANISMS); BOMBYX MORI; PSEUDOMONAS AERUGINOSA;

EID: 80054841862     PISSN: 08824010     EISSN: 10961208     Source Type: Journal    
DOI: 10.1016/j.micpath.2011.09.002     Document Type: Article

References (45)

1. Hazlett L.D. Bacterial infections of the cornea (Pseudomonas aeruginosa). Chem Immunol Allergy 2007, 92:185-194.
2. Takeyama K., Kunishima Y., Matsukawa M., Takahashi S., Hirose T., Kobayashi N., et al. Multidrug-resistant Pseudomonas aeruginosa isolated from the urine of patients with urinary tract infection. J Infect Chemother 2002, 8:59-63.
3. Prithiviraj B., Weir T., Bais H.P., Schweizer H.P., Vivanco J.M. Plant models for animal pathogenesis. Cell Microbiol 2005, 7:315-324.
4. Cosson P., Zulianello L., Join-Lambert O., Faurisson F., Gebbie L., Benghezal M., et al. Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system. J Bacteriol 2002, 184:3027-3033.
5. D'Argenio D.A., Gallagher L.A., Berg C.A., Manoil C. Drosophila as a model host for Pseudomonas aeruginosa infection. J Bacteriol 2001, 183:1466-1471.
6. Kurz C.L., Ewbank J.J. Caenorhabditis elegans for the study of host-pathogen interactions. Trends Microbiol 2000, 8:142-144.
7. Miyata S., Casey M., Frank D.W., Ausubel F.M., Drenkard E. Use of the Galleria mellonella caterpillar as a model host to study the role of the type III secretion system in Pseudomonas aeruginosa pathogenesis. Infect Immun 2003, 71:2404-2413.
8. Rahme L.G., Tan M.W., Le L., Wong S.M., Tompkins R.G., Calderwood S.B., et al. Use of model plant hosts to identify Pseudomonas aeruginosa virulence factors. Proc Natl Acad Sci USA 1997, 94:13245-13250.
9. Scully L.R., Bidochka M.J. Developing insect models for the study of current and emerging human pathogens. FEMS Microbiol Lett 2006, 263:1-9.
10. Goldberg J.B., Dahnke T. Pseudomonas aeruginosa AlgB, which modulates the expression of alginate, is a member of the NtrC subclass of prokaryotic regulators. Mol Microbiol 1992, 6:59-66.
11. Hendrickson E.L., Plotnikova J., Mahajan-Miklos S., Rahme L.G., Ausubel F.M. Differential roles of the PA14 rpoN gene in pathogenicity in plants, nematodes, insects, and mice. J Bacteriol 2001, 183:7126-7134.
12. Totten P.A., Lara J.C., Lory S. The rpoN gene product of Pseudomonas aeruginosa is required for expression of diverse genes, including the flagellin gene. J Bacteriol 1990, 172:389-396.
13. Yorgey P., Rahme L.G., Tan M.W., Ausubel F.M. The roles of mucD and alginate in the virulence of Pseudomonas aeruginosa in plants, nematodes and mice. Mol Microbiol 2001, 41:1063-1076.
14. Rahme L.G., Stevens E.J., Wolfort S.F., Shao J., Tompkins R.G., Ausubel F.M. Common virulence factors for bacterial pathogenicity in plants and animals. Science 1995, 268:1899-1902.
15. Tan M.W., Mahajan-Miklos S., Ausubel F.M. Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis. Proc Natl Acad USA 1999, 96:715-720.
16. Chieda Y., Iiyama K., Yasunaga-Aoki C., Lee J.M., Kusakabe T., Shimizu S. Pathogenicity of gacA mutant of Pseudomonas aeruginosa PA01 in the silkworm, Bombyx mori. FEMS Microbiol Lett 2005, 244:181-186.
17. Iiyama K., Chieda Y., Lee J.M., Kusakabe T., Yasunaga-Aoki C., Shimizu S. Effect of superoxide dismutase gene inactivation on virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori. Appl Environ Microbiol 2007, 73:1569-1575.
18. Chieda Y., Iiyama K., Lee J.M., Kusakabe T., Yasunaga-Aoki C., Shimizu S. Inactivation of pyocyanin synthesis genes has no effect on the virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori. FEMS Microbiol Lett 2008, 278:101-107.
19. Iglewski B.H., Kabat D. NAD-dependent inhibition of protein synthesis by Pseudomonas aeruginosa toxin. Proc Natl Acad Sci USA 1975, 72:2284-2288.
20. Hirakata Y., Furuya N., Tateda K., Kaku M., Yamaguchi K. In vivo production of exotoxin A and its role in endogenous Pseudomonas aeruginosa septicemia in mice. Infect Immun 1993, 61:2468-2473.
21. Pillar C.M., Hobden J.A. Pseudomonas aeruginosa exotoxin A and keratitis in mice. Invest Ophthalmol Vis Sci 2002, 43:1437-1444.
22. Miyazaki S., Matsumoto T., Tateda K., Ohno A., Yamaguchi K. Role of exotoxin A in inducing severe Pseudomonas aeruginosa infections in mice. J Med Microbiol 1995, 43:169-175.
23. Blackwood L.L., Stone R.M., Iglewski B.H., Pennington J.E. Evaluation of Pseudomonas aeruginosa exotoxin and elastase as virulence factors in acute lung infection. Infect Immun 1983, 39:198-201.
24. Schultz M.J., Rijneveld A.W., Florquins S., Speelman P., van Deventer S.J.H., Paul T.D.V. Impairment of host defense by exotoxin A in Pseudomonas aeruginosa pneumonia in mice. J Med Microbiol 2001, 50:820-827.
25. Woods D.E., Cryz S.J., Friedmass R.L., Iglewski B.H. Contribution of toxin A and elastase to virulence of Pseudomonas aeruginosa in chronic lung infections of rats. Infect Immun 1982, 37:1223-1228.
26. Hossain M.S., Hamamoto H., Matsumoto Y., Razanajatovo I.M., Larranaga J., Kaito C., et al. Use of silkworm larvae to study pathogenic bacterial toxins. J Biochem 2006, 140:439-444.
27. Tanaka H., Yamakawa M. Regulation of the innate immune responses in the silkworm, Bombyx mori. Invert Surviv J 2011, 8:59-69.
28. Hultmark D., Steiner H., Rasmuson T., Boman H.G. Insect immunity. Purification and properties of three inducible bacteriocidal proteins from hemolymph of immunized pupae of Hyalophora cecropia. Eur J Biochem 1980, 106:7-16.
29. Chen T., Zhao P., Liu C., Xu P., Gao Z., Xia Q., et al. Structures, regulatory regions, and inductive expression patterns of antimicrobial peptide genes in the silkworm Bombyx mori. Genomics 2006, 87:356-365.
30. Vijendravarma R.K., Godfray H.C., Kraaijeveld A.R. Infection of Drosophila melanogaster by Tubulinosema kingi: stage-specific susceptibility and within-host proliferation. J Invertebr Pathol 2008, 99:239-241.
31. Ekesi S., Maniania N.K. Susceptibility of Megalurothrips sjostedti developmental stages to Metarhizium anisopliae and the effects of infection on feeding, adult fecundity, egg fertility and longevity. Entomol Exp Appl 2000, 94:229-236.
32. Ansari M.A., Adhikari B.N., Ali F., Moens M. Susceptibility of Hoplia philanthus (Coleoptera: Scarabaeidae) larvae and pupae to entomopathogenic nematodes (Rhabditida: Steinernematidae, Heterorhabditidae). Biol Control 2008, 47:315-321.
33. Tian L., Guo E., Diao Y., Zhou S., Peng Q., Cao Y., et al. Genome-wide regulation of innate immunity by juvenile hormone and 20-hydroxyecdysone in the Bombyx fat body. BMC Genomics 2010, 11:549.
34. Chieda Y., Iiyama K., Lee J.M., Kusakabe T., Yasunaga-Aoki C., Shimizu S. The gacA gene of Pseudomonas aeruginosa PAO1 is not required for full virulence in Bombyx mori. J Insect Biotechnol Sericology 2007, 76:89-95.
35. Tanada Y., Kaya H.K. Amicrobial and microbial agents. I. Latent, chronic, and acute infection. Insect pathology 1993, 72. Academic Press, Inc., USA.
36. Schweizer H.P. The agmR gene, an environmentally responsive gene, complements defective glpR, which encodes the putative activator for glycerol metabolism in Pseudomonas aeruginosa. J Bacteriol 1991, 173:6798-6806.
37. Watson A.A., Richard A.A., Mattick J.S. Construction of improved vectors for protein production in Pseudomonas aeruginosa. Gene 1996, 172:163-164.
38. Cytryńska M., Mak P., Zdybicka-Barabas A., Suder P., Jakubowicz T. Purification and characterization of eight peptides from Galleria mellonella immune hemolymph. Peptides 2007, 28:533-546.
39. Sambrook J., Fritsch E.F., Maniatis T. Molecular cloning: a laboratory manual 1989, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 2nd ed.
40. Simon R., Priefer U., Pühler A. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio/Technology 1983, 1:784-794.
41. Kovach M.E., Elzer P.H., Hill D.S., Robertson G.T., Farris M.A., Roop R.M., et al. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 1995, 166:175-176.
42. Schäfer A., Tauch A., Jäger W., Kalinowski J., Thierbach G., Pühler A. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 1994, 145:69-73.
43. Blondelet-Rouault M.H., Weiser J., Lebrihi A., Branny P., Pernodet J.L. Antibiotic resistance gene cassettes derived from the Ω interposon for use in E. coli and Streptomyces. Gene 1997, 190:315-317.
44. Prentki P., Krisch H.M. In vitro insertional mutagenesis with a selectable DNA fragment. Gene 1984, 29:303-313.
45. Kimura Y., Iwaki M., Lim C.R., Kohno K. A novel mutation which enhances the fluorescence of green fluorescent protein at high temperatures. Biochem Biophys Res Commun 1997, 232:69-73.