Two-Dimensional Electrophoresis in Proteomics

Protein Purification: Principles, High Resolution Methods, and Applications: Third Edition

Volumn , Issue , 2011, Pages 411-439

  Westermeier, Reiner ^a   Görg, Angelika ^b  

^a SERVA Electrophoresis GmbH   (Germany)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

SDS electrophoresis second dimension SDS PAGE, performed in the discontinuous tris chloride glycine buffer system, in presence of 0.1 SDS; SDS Polyacrylamide Gel Electrophoresis (SDS PAGE); Two dimensional electrophoresis in proteomics

Indexed keywords

CHLORINE COMPOUNDS; MOLECULAR BIOLOGY;

BUFFER SYSTEM; PROTEOMICS; SDS PAGE; TWO DIMENSIONAL ELECTROPHORESIS;

ELECTROPHORESIS;

PROTEIN; PROTEOME;

ARTICLE; HUMAN; INSTRUMENTATION; ISOELECTRIC FOCUSING; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METHODOLOGY; PH; PROTEOMICS; TUMOR CELL LINE; TWO DIMENSIONAL GEL ELECTROPHORESIS;

CELL LINE, TUMOR; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; PROTEINS; PROTEOME; PROTEOMICS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 80054809009     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780470939932.ch17     Document Type: Chapter

References (72)

1. J. Margolis, K.G. Kenrick, Nature (London), 221, 1056-1057 (1969).
2. V. Macko, H. Stegemann, Hoppe-Seyler's Z. Physiol. Chem., 350, 917-919 (1969).
3. P.H. O'Farrell, J. Biol. Chem., 250, 4007-4021 (1975).
4. J. Klose, Humangenetik, 26, 231-243 (1975).
5. D. MacFarlane, Anal. Biochem., 176, 457-463 (1989).
6. A. Görg, W. Postel, S. Günther, Electrophoresis, 9, 531-546 (1988).
7. V.C. Wasinger, S.J. Cordwell, A. Cerpa-Poljak, J.X. Yan, A.A. Gooley, M.R. Wilkins, M.W. Duncan, R. Harris, K.L. Williams, I. Humphery-Smith, Electrophoresis, 16, 1090-1094 (1995).
8. A. Koller, M.P. Washburn, B.M. Lange, N.L. Andon, C. Deciu, P.A. Haynes, L. Hays, D. Schieltz, R. Ulaszek, J. Wei, D. Wolters, J.R. Yates III. Proc. Natl Acad. Sci. USA, 99, 11969-11974 (2002).
9. M. Ünlü, M.E. Morgan, J.S. Minden, Electrophoresis, 18, 2071-2077, (1997).
10. P.Z. O'Farrell, H.M. Goodman, P.H. O'Farrell, Cell, 12, 1133-1142 (1977).
11. B. Bjellqvist, K. Ek, P.G. Righetti, E. Gianazza, A. Görg, R. Westermeier, W. Postel, J. Biochem. Biophys. Methods, 6, 317-339 (1982).
12. A. Görg, G. Bogüth, C. Obermaier, A. Posch, W. Weiss, Electrophoresis, 16, 1079-1086 (1995).
13. J. Parness, K. Paul-Pletzer, Anal. Biochem., 289, 98-99 (2001).
14. A. Görg, C. Obermaier, G. Bogüth, A. Harder, B. Scheibe, R. Wildgruber, W. Weiss, Electrophoresis, 21, 1037-1053 (2000).
15. C. Damerval, D. DeVienne, M. Zivy, H. Thiellement, Electrophoresis, 7, 53-54 (1986).
16. R. Wildgruber, G. Reil, O. Drews, H. Parlar, A. Görg, Proteomics, 2, 727-732 (2002).
17. T. Rabilloud, Electrophoresis, 19, 758-760 (1998).
18. I. Olsson, K. Larsson, R. Palmgren, B. Bjellqvist, Proteomics, 2, 1630-1632 (2002).
19. V. Méchin, L. Consoli, M. Le Guilloux, C. Damerval, Proteomics, 3, 1299-1302 (2003).
20. D. Wessel, U.I. Flügge, Anal. Biochem., 138, 141-143 (1984).
21. T. Stasyk, U. Hellman, S. Souchelnytskyi, Life Sci. News, 9, 9-12 (2001).
22. Ettan DIGE. User Manual. GE Healthcare Life Sciences No. 18-1164-40.
23. J. Shaw, R. Rowlinson, J. Nickson, T. Stone, A. Sweet, K. Williams, R. Tonge, Proteomics, 3, 1181-1195 (2003).
24. CyDye DIGE fluor labelling kit for scarce samples. Instruction. GE Healthcare Life Sciences. No. 25800983PL (2003).
25. M.L. Ramsby, G.S. Makowski, E.A. Khairallah, Electrophoresis, 15, 265-277 (1994).
26. L.A. Huber, K. Pfaller, I. Vietor, Circ. Res., 92, 962-968 (2003).
27. T.G. Pretlow, T.P. Pretlow, Methods: A Companion to Methods in Enzymology, 2, 183-191 (1991).
28. H. Zischka, G. Weber, P.J.A. Weber, A. Posch, R.J. Braun, D. Bühringer, U. Schneider, M. Nissum, T. Meitinger, M. Ueffing, C. Eckerskorn, Proteomics, 3, 906-916 (2003).
29. N.L. Anderson, N.G. Anderson, Mol. Cell Proteomics, 1, 845-867 (2002).
30. H. Schägger, G. von Jagow, Anal. Biochem., 199, 223-231 (1991).
31. W. Werhahn, H.-P. Braun, Electrophoresis, 23, 640-646 (2002).
32. P. Wenger, M. de Zuanni, P. Javet, P.G. Righetti, J. Biochem. Biophys. Methods, 14, 29-43 (1987).
33. B. Herbert, P.G. Righetti, Electrophoresis, 21, 3639-3648 (2000).
34. D.W. Speicher, X. Zuo, Anal. Biochem, 284, 266-278 (2000).
35. A. Görg, G. Boguth, A. Köpf, G. Reil, H. Parlar, W. Weiss, Proteomics, 2, 1652-1657 (2002).
36. A. Blomberg, L. Blomberg, J. Norbeck, S.J. Fey, P.M. Larsen, P. Roepstorff, H. Degand, M. Boutry, A. Posch, A. Görg, Electrophoresis, 16, 1935-1945 (1995).
37. J. Asakawa, Electrophoresis, 9, 562-568 (1988).
38. D. Hochstrasser, V. Augsburger, M. Funk, R. Appel, C. Pellegrini, A.F. Muller, In Electrophoresis '86, M.J. Dunn, ed., VCH, Weinheim, 1986, pp.566-568.
39. A. Görg, In Cell Biology. A Laboratory Handbook. Volume 4, J.E. Celis, ed., Academic Press Inc., San Diego, CA. 1998, pp. 386-397.
40. A. Görg, W. Weiss, In Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods, T. Rabilloud, ed., Springer, Berlin Heidelberg, New York, 2000, pp. 57-106.
41. R. Westermeier, W. Postel, J. Weser, A. Görg, J. Biochem. Biophys. Methods, 8, 321-330 (1983).
42. A. Görg, W. Postel, J. Weser, S. Günter, J.R. Strahler, S.M. Hanash, L. Somerlot, Electrophoresis, 8, 45-51 (1987).
43. T. Rabilloüd, C. Valette, J.J. Lawrence, Electrophoresis, 15, 1552-1558 (1994).
44. A. Görg, G. Bogüth, C. Obermaier, A. Harder, W. Weiss, Life Sci. News, 1, 4-6 (1998).
45. F. Sabounchi-Schütt, J. Aström, I. Olsson, A. Eklund, J. Grünewald, B. Bjellqvist, Electrophoresis, 21, 3649-3656 (2000).
46. A. Shevchenko, M. Wilm, O. Vorm, M. Mann, Anal. Chem., 68, 850-858 (1996).
47. B. Bjellqvist, J.-C. Sanchez, C. Pasquali, F. Ravier, N. Paqüet, S. Frutiger, G.J. Hughes, D. Hochstrasser, Electrophoresis, 14, 1375-1378 (1993).
48. R. Westermeier, T. Naven, Proteomics in Practice. A Laboratory Manual of Proteome Analysis, Wiley-VCH, Weinheim, 2002.
49. A. Görg, W. Postel, C. Friedrich, R. Kuick, J.R. Strahler, S.M. Hanash. Electrophoresis, 12, 653-658 (1991).
50. A. Görg, C. Obermaier, G. Bogüth, W. Weiss, Electrophoresis, 20, 712-717 (1999).
51. O. Drews, G. Reil, H. Parlar, A. Görg, Proteomics, 4, 1293-1304 (2004).
52. N.P. Patestos, M. Fauth, B.J. Radola, Electrophoresis, 9, 488-496 (1988).
53. A. Görg, W. Postel, J. Weser, S. Günther, J.R. Strahler, S.M. Hanash, L. Somerlot, Electrophoresis, 8, 122-124 (1987).
54. U.K. Lämmli, Nature, 227, 680-685 (1970).
55. R. Westermeier. Electrophoresis in Practice. A Guide to Theory and Practice. 4th edn, Wiley-VCH, Weinheim, 2004.
56. N.G. Anderson, N.L. Anderson, Anal. Biochem., 85, 341-354 (1978).
57. J. Heükeshoven, R. Dernick, Electrophoresis, 6, 103-112 (1985).
58. J. Heükeshoven, R. Dernick, Electrophoresis, 9, 60-61 (1988).
59. E. Hardy, H. Santana, A.E. Sosa, L. Hernandez, C. Fernandez- Patron, L. Castellanos-Serra, Anal. Biochem., 240, 150-152 (1996).
60. V. Neuhoff, N. Arold, D. Taube, W. Ehrhardt, Electrophoresis, 9, 255-262 (1988).
61. C.R. Merril, R.C. Switzer, M.L. Van Keuren, Proc. Natl Acad. Sci. USA, 76, 4335-4339 (1979).
62. H. Blum, H. Beier, H.J. Gross, Electrophoresis, 8, 93-99 (1987).
63. A. Shevchenko, M. Wilm, O. Vorm, M. Mann, Anal. Chem., 68, 850-858 (1996).
64. J.A. Mackintosh, H.-Y. Choi, S.-H. Bae, D.A. Veal, P.J. Bell, A.C. Ferrari, D.D. Van Dyk, N.M. Verrills, Y.-K. Paik, P. Karuso, Proteomics, 3, 2273-2288 (2003).
65. K.N. Berggren, B. Schulenberg, M.F. Lopez, T.H. Steinberg, A. Bogdanova, G. Smejkal, A. Wang, W.F. Patton, Proteomics, 2, 486-498 (2002).
66. W.M. Bonner, R.A. Laskey, Eur. J. Biochem., 46, 83-88 (1974).
67. J.P. Chamberlain, Anal. Biochem., 98, 132-135 (1979).
68. GE Healthcare Life Sciences, Datafile: Immobiline DryStrip visualization of pH gradients. 18-1140-60 (2000).
69. B. Bjellqvist, J.-C. Sanchez, C. Pasquali, F. Ravier, N. Paquet, S. Frutiger, G.J. Hughes, D. Hochstrasser, Electrophoresis, 14, 1375-1378 (1993).
70. R.J. Simpson, Proteins and Proteomics. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2003.
71. R. Westermeier, T. Naven, H.-R. Hoepker, Proteomics in Practice. 4th edn, Wiley-VCH, Weinheim, 2008.
72. T. Keough, R.S. Youngquist, M.P. Lacey, Proc. Natl Acad. Sci. USA, 96, 7131-7136 (1999).