Quantification of PARP Activity in Human Tissues: Ex Vivo Assays in Blood Cells and Immunohistochemistry in Human Biopsies

Methods in Molecular Biology

Volumn 780, Issue , 2011, Pages 267-275

  Horvath, Eszter M ^a   Zsengellér, Zsuzsanna K ^b   Szabo, Csaba ^c  

^a SEMMELWEIS UNIVERSITY   (Hungary)

^b HARVARD MEDICAL SCHOOL   (United States)

^c University of Texas Medical Branch School of Medicine   (United States)

Author keywords

Biopsy; Circulating leukocytes; DAB; Immunohistochemistry; PARP; Poly(ADP ribose)

Indexed keywords

NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; POLY(ADENOSINE DIPHOSPHATE RIBOSE);

ARTICLE; BLOOD CELL; ENZYME ASSAY; HUMAN; IMMUNOHISTOCHEMISTRY; METABOLISM; METHODOLOGY;

BLOOD CELLS; ENZYME ASSAYS; HUMANS; IMMUNOHISTOCHEMISTRY; POLY ADENOSINE DIPHOSPHATE RIBOSE; POLY(ADP-RIBOSE) POLYMERASES;

EID: 80054771433     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-270-0_16     Document Type: Chapter

References (9)

1. Schreiber V, Dantzer F, Ame JC, de Murcia G (2006) Poly(ADP-ribose): novel functions for an old molecule. Nat Rev Mol Cell Biol 7: 517–528
2. Jagtap P, Szabo C (2005) Poly(ADP-ribose) polymerase and the therapeutic effects of its inhibitors. Nat Rev Drug Discov 4:421–440
3. Virag L, Szabo C (2002) The therapeutic potential of poly(ADP-ribose) polymerase inhibitors. Pharmacol Rev 54:375–429
4. Lazebnik YA, Kaufmann SH, Desnoyers S, Poirier GG, Earnshaw WC (1994) Cleavage of poly (ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371:346–347
5. Ame JC, Spenlehauer C, de Murcia G (2004) The PARP superfamily. BioEssays 26:882–893
6. de Murcia JM, Niedergang C, Trucco C, Ricoul M, Dutrillaux B, Mark M, Oliver FJ, Masson M, Dierich A, LeMeur M, Walztinger C, Chambon P, de Murcia G (1997) Requirement of poly(ADP-ribose) polymerase in recovery from DNA damage in mice and in cells. Proc Natl Acad Sci USA 94:7303–7307
7. Pacher P, Liaudet L, Soriano FG, Mabley JG, Szabo E, Szabo C (2002) The role of poly(ADP-ribose) polymerase activation in the development of myocardial and endothelial dysfunction in diabetes. Diabetes 51:514–521
8. Tóth-Zsámboki E, Horváth E, Vargova K, Pankotai E, Murthy K, Zsengellér Z, Bárány T, Pék T, Fekete K, Kiss RG, Préda I, Lacza Z, Gerö D, Szabó C (2006) Activation of poly(ADP-ribose) polymerase by myocardial ischemia and coronary reperfusion in human circulating leukocytes. Mol Med 12:221–228
9. Horváth EM, Magenheim R, Kugler E, Vácz G, Szigethy A, Lévárdi F, Kollai M, Szabo C, Lacza Z (2009) Nitrative stress and poly(ADP-ribose) polymerase activation in healthy and gestational diabetic pregnancies. Diabetologia 14:1935–1943