MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs

Methods in Molecular Biology

Volumn 784, Issue , 2011, Pages 99-107

  Hennessy, Erica ^a   O’Driscoll, Lorraine ^b  

^a DUBLIN CITY UNIVERSITY   (Ireland)

^b TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

Megaplex RT primers; MicroRNA; miRNA; TaqMan low density microRNA array

Indexed keywords

MICRORNA;

ARTICLE; CELL LINE; DNA MICROARRAY; EXTRACELLULAR SPACE; GENE EXPRESSION PROFILING; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

CELL LINE; EXTRACELLULAR SPACE; GENE EXPRESSION PROFILING; HUMANS; MICRORNAS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 80054756673     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-289-2_7     Document Type: Chapter

References (8)

1. Bartel, D. P. (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116, 281–97.
2. Zamore, P. D., and Haley, B. (2005) Ribo-gnome: the big world of small RNAs. Science 309, 1519–24.
3. Hennessy, E., and O’Driscoll, L. (2008) Molecular medicine of microRNAs: structure, function and implications for diabetes. Expert Rev Mol Med 10, e24.
4. Chomczynski, P., and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidin-ium thiocyanate-phenol-chloroform extraction. Anal Biochem 162, 156–9.
5. Livak, K. J., and Schmittgen, T. D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods 25, 402–8.
6. Griffiths-Jones, S., Saini, H. K., van Dongen, S., and Enright, A. J. (2008) miRBase: tools for microRNA genomics. Nucleic Acids Res 36, D154–8.
7. Griffiths-Jones, S., Grocock, R. J., van Dongen, S., Bateman, A., and Enright, A. J. (2006) miRBase: microRNA sequences, targets and gene nomenclature. Nucleic Acids Res 34, D140–4.
8. Griffiths-Jones, S. (2004) The microRNRegistry. Nucleic Acids Res 32, D109–11.