Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex

Methods in Molecular Biology

Volumn 793, Issue , 2011, Pages 63-75

  Kim, Hyun Jeong ^a   Magrané, Jordi ^a  

^a WEILL CORNELL MEDICAL COLLEGE   (United States)

Author keywords

Astrocytes; Cellular markers; Coating; Cortical neurons; Immunofluorescence

Indexed keywords

ANIMALIA; RODENTIA;

ANIMAL; ARTICLE; ASTROCYTE; BRAIN CORTEX; CELL SEPARATION; CULTURE TECHNIQUE; CYTOLOGY; DEGENERATIVE DISEASE; DISSECTION; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; GENETIC TRANSFECTION; GLIA; METABOLISM; METHODOLOGY; MOUSE; NERVE CELL; PATHOLOGY; PREGNANCY;

ANIMALS; ASTROCYTES; CELL CULTURE TECHNIQUES; CELL SEPARATION; CEREBRAL CORTEX; DISSECTION; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE; MICE; NEURODEGENERATIVE DISEASES; NEUROGLIA; NEURONS; PREGNANCY; TRANSFECTION;

EID: 80054744496     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-328-8_4     Document Type: Chapter

References (18)

1. Dotti CG, Sullivan CA, Banker GA (1988) The establishment of polarity by hippocampal neurons in culture. J. Neurosci. 8, 1454–68.
2. Fletcher TL, Banker G (1089) The establishment of polarity by hippocampal neurons: the relationship between the stage of a cell’s development in situ and its subsequent development in culture. Dev. Biol. 136, 446–455.
3. Craig AM, Graf ER, Linhoff MW (2006) How to build a central synapse: clues from cell culture. Trends Neurosci. 29, 8–20.
4. Atluri PP, Ryan TA (2006) The kinetics of synaptic vesicle reacidification at hippocampal nerve terminals. J. Neurosci. 26, 2313–2320.
5. Araque A, Carmignoto G, Haydon PG (2001) Dynamic signaling between astrocytes and neurons. Annu. Rev. Physiol. 63, 795–813.
6. Doetsch F (2003) The glial identity of neural stem cells. Nat. Neurosci. 6, 1127–1134.
7. Fields RD, Stevens-Graham B (2002) New insights into neuro-glia communication. Science. 298, 556–562.
8. Janzer RC (1993) The blood-brain barrier: cellular basis. J. Inherit. Metab. Dis. 16, 639–647.
9. Montgomery DL (1994) Astrocytes: form, functions, and roles in disease. Vet. Pathol. 31, 145–167.
10. Parri R, Crunelli V (2003) An astrocyte bridge from synapse to blood flow. Nat. Neurosci. 6, 5–6.
11. Verkhratsky A, Toescu EC (2006) Neuronal-glial networks as substrate for CNS integration. J. Cell. Mol. Med. 10(4), 826–836.
12. Eriksson PS, Perfilieva E, Björk-Eriksson T, Albron AM, Nordborg C, Peterson ST, Gage FH (1998) Neurogenesis in the adult human hippocampus. Nat. Med. 4(11), 1313–1317.
13. Luebke JI, Weaver CM, Rocher AB, Rodriguez A, Crimins JL, Dickstein DL, Wearne SL, Hof PR (2010) Dendritic vulnerability in neurodegenerative disease: insights from analyses of cortical pyramidal neurons in transgenic mouse models. Brain Struct. Funct. 214, 181–99.
14. Rozas JL, Gómez-Sánchez L, Tomás-Zapico C, Lucas JJ, Fernández-Chacón R (2010) Presynaptic dysfunction in Huntington’s disease. Biochem. Soc. Trans. 38, 488–92.
15. Knobloch M, Mansuy IM (2008) Dendritic spine loss and synaptic alterations in Alzheimer’s disease. Mol Neurobiol. 37, 73–82.
16. Dong Y, Benveniste EN (2001) Immune function of astrocytes. Glia. 36, 180–190.
17. Sofroniew MW (2005) Reactive astrocytes in neuronal repair and protection. Neuroscientist. 11, 400–407.
18. McCarthy KD, de Vellis J (1980) Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J. Cell. Biol. 85, 890–902.