Phosphoprotein Stability in Clinical Tissue and Its Relevance for Reverse Phase Protein Microarray Technology

Methods in Molecular Biology

Volumn 785, Issue , 2011, Pages 23-43

  Espina, Virginia ^a   Mueller, Claudius ^a   Liotta, Lance A ^a  

^a GEORGE MASON UNIVERSITY   (United States)

Author keywords

Cell signaling; Kinase; Phopshoprotein; Pre analytical variablity; Reverse phase protein microarray; Stability

Indexed keywords

BIOLOGICAL MARKER; FORMALDEHYDE; PHOSPHOPROTEIN; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN KINASE;

ARTICLE; CELL COMMUNICATION; LABORATORY DIAGNOSIS; METABOLISM; METHODOLOGY; PHYSIOLOGY; PROTEIN MICROARRAY; PROTEIN STABILITY; SIGNAL TRANSDUCTION;

BIOLOGICAL MARKERS; CELL COMMUNICATION; FORMALDEHYDE; PHOSPHOPROTEIN PHOSPHATASES; PHOSPHOPROTEINS; PROTEIN ARRAY ANALYSIS; PROTEIN KINASES; PROTEIN STABILITY; SIGNAL TRANSDUCTION; SPECIMEN HANDLING;

EID: 80054737693     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-286-1_3     Document Type: Chapter

References (28)

1. Espina, V., Edmiston, K. H., Heiby, M., Pierobon, M., Sciro, M., et al. (2008) A portrait of tissue phosphoprotein stability in the clinical tissue procurement process. Mol Cell Proteomics 7, 1998–2018.
2. Li, X., Friedman, A. B., Roh, M. S., and Jope, R. S. (2005) Anesthesia and post-mortem interval profoundly influence the regulatory serine phosphorylation of glycogen synthase kinase-3 in mouse brain. J Neurochem 92, 701–4.
3. Li, J., Gould, T. D., Yuan, P., Manji, H. K., and Chen, G. (2003) Post-mortem interval effects on the phosphorylation of signaling proteins. Neuropsychopharmacology 28, 1017–25.
4. Becker, K. F., Schott, C., Hipp, S., Metzger, V., Porschewski, P., et al. (2007) Quantitative protein analysis from formalin-fixed tissues: implications for translational clinical research and nanoscale molecular diagnosis. J Pathol 211, 370–8.
5. Fox, C. H., Johnson, F. B., Whiting, J., and Roller, P. P. (1985) Formaldehyde fixation. J Histochem Cytochem 33, 845–53.
6. Helander, K. G. (1994) Kinetic studies of formaldehyde binding in tissue. Biotech Histochem 69, 177–9.
7. Srinivasan, M., Sedmak, D., and Jewell, S. (2002) Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am J Pathol 161, 1961–71.
8. Nassiri, M., Ramos, S., Zohourian, H., Vincek, V., Morales, A. R., et al. (2008) Preservation of biomolecules in breast cancer tissue by a form-alin-free histology system. BMC Clin Pathol 8, 1.
9. Devireddy, R. V. (2005) Predicted permeability parameters of human ovarian tissue cells to various cryoprotectants and water. Mol Reprod Dev 70, 333–43.
10. He, Y., and Devireddy, R. V. (2005) An inverse approach to determine solute and solvent permeability parameters in artificial tissues. Ann Biomed Eng 33, 709–18.
11. Goldstein, B. J. (2002) Protein-tyrosine phosphatases: emerging targets for therapeutic intervention in type 2 diabetes and related states of insulin resistance. J Clin Endocrinol Metab 87, 2474–80.
12. Neel, B. G., and Tonks, N. K. (1997) Protein tyrosine phosphatases in signal transduction. Curr Opin Cell Biol 9, 193–204.
13. Grellner, W., Vieler, S., and Madea, B. (2005) Transforming growth factors (TGF-alpha and TGF-beta1) in the determination of vitality and wound age: immunohistochemical study on human skin wounds. Forensic Sci Int 153, 174–80.
14. Grellner, W. (2002) Time-dependent immunohistochemical detection of proinflammatory cytokines (IL-1beta, IL-6, TNF-alpha) in human skin wounds. Forensic Sci Int 130, 90–6.
15. Grellner, W., and Madea, B. (2007) Demands on scientific studies: vitality of wounds and wound age estimation. Forensic Sci Int 165, 150–4.
16. Ohshima, T. (2000) Forensic wound examination. Forensic Sci Int 113, 153–64.
17. Oehmichen, M. (2004) Vitality and time course of wounds. Forensic Sci Int 144, 221–31.
18. Paweletz, C. P., Charboneau, L., Bichsel, V. E., Simone, N. L., Chen, T., et al. (2001) Reverse phase protein microarrays which capture disease progression show activation of pro-survival pathways at the cancer invasion front. Oncogene 20, 1981–9.
19. Petricoin, E. F., 3rd, Espina, V., Araujo, R. P., Midura, B., Yeung, C., et al. (2007) Phosphoprotein pathway mapping: Akt/mam-malian target of rapamycin activation is negatively associated with childhood rhabdomyosarcoma survival. Cancer Res 67, 3431–40.
20. VanMeter, A., Signore, M., Pierobon, M., Espina, V., Liotta, L. A., et al. (2007) Reverse-phase protein microarrays: application to biomarker discovery and translational medicine. Expert Rev Mol Diagn 7, 625–33.
21. Wulfkuhle, J. D., Speer, R., Pierobon, M., Laird, J., Espina, V., et al. (2008) Multiplexed cell signaling analysis of human breast cancer applications for personalized therapy. J Proteome Res 7, 1508–17.
22. Espina, V., Mehta, A. I., Winters, M. E., Calvert, V., Wulfkuhle, J., et al. (2003) Protein microarrays: molecular profiling technologies for clinical specimens. Proteomics 3, 2091–100.
23. Belluco, C., Mammano, E., Petricoin, E., Prevedello, L., Calvert, V., et al. (2005) Kinase substrate protein microarray analysis of human colon cancer and hepatic metastasis. Clin Chim Acta 357, 180–3.
24. Berggren, K., Steinberg, T. H., Lauber, W. M., Carroll, J. A., Lopez, M. F., et al. (1999) A luminescent ruthenium complex for ultrasensi-tive detection of proteins immobilized on membrane supports. Anal Biochem 276, 129–43.
25. Berggren, K. N., Schulenberg, B., Lopez, M. F., Steinberg, T. H., Bogdanova, A., et al. (2002) An improved formulation of SYPRO Ruby protein gel stain: comparison with the original formulation and with a ruthenium II tris (bathophenanthroline disulfonate) formulation. Proteomics 2, 486–98.
26. Espina, V., Wulfkuhle, J. D., Calvert, V. S., VanMeter, A., Zhou, W., et al. (2006) Laser-capture microdissection. Nat Protoc 1, 586–603.
27. Stillman, B. A., and Tonkinson, J. L. (2000) FAST slides: a novel surface for microarrays. Biotechniques 29, 630–5.
28. Tonkinson, J. L., and Stillman, B. A. (2002) Nitrocellulose: a tried and true polymer finds utility as a post-genomic substrate. Front Biosci 7, c1–12.