Real-time PCR to assess the Leishmania load in Lutzomyia longipalpis sand flies: Screening of target genes and assessment of quantitative methods

Experimental Parasitology

Volumn 129, Issue 3, 2011, Pages 234-239

  Bezerra Vasconcelos, Diana R ^a   Melo, Luciana M ^a   Albuquerque, Érica S ^a   Luciano, Maria C S ^a   Bevilaqua, Claudia M L ^a  

^a STATE UNIVERSITY OF CEARÁ   (Brazil)

Author keywords

Leishmania infantum chagasi; Lutzomyia longipalpis; Molecular targets; PCR

Indexed keywords

18S RIBOSOMAL SUBUNIT GENE; ACCURACY; ARTICLE; CONTROLLED STUDY; DNA EXTRACTION; DNA POLYMERASE ALPHA GENE; GENE; GENE DOSAGE; GENETIC SCREENING; KDNA GENE; LEISHMANIA; LEISHMANIA INFANTUM CHAGASI; LUTZOMYIA; LUTZOMYIA LONGIPALPIS; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PARASITE LOAD; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; V ATP GENE;

ANIMALS; DNA PRIMERS; DNA, KINETOPLAST; DNA, PROTOZOAN; DNA, RIBOSOMAL; INSECT VECTORS; LEISHMANIA; MALE; PSYCHODIDAE; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT;

LEISHMANIA INFANTUM; LUTZOMYIA LONGIPALPIS; PHLEBOTOMINAE;

EID: 80054737340     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2011.08.010     Document Type: Article

References (30)

1. Bauzer L.G., Souza N.A., Ward R.D., Kyriacou C.P., Peixoto A.A. The period gene and genetic differentiation between three Brazilian populations of Lutzomyia longipalpis. Insect Molecular Biology 2002, 11:315-323.
2. Bretagne S., Durand R., Olivi M., Garin J.F., Sulahian A., Rivollet D., Vidaud M., Deniau M. Real-time PCR as a new tool for quantifying Leishmania infantum in liver in infected mice. American Society for Microbiology 2001, 8:828-831.
3. Clements D.N., Wood S., Carter S.D., Ollier W.E.R. Assessment of the quality and quantity of genomic DNA recovered from canine blood samples by three different extraction methods. Research in Veterinary Science 2008, 85:74-79.
4. Dantas-Torres F., Brandão-Filho S.P. Visceral Leishmaniasis in Brazil: revisiting paradigms of epidemiology and control. Revista do Instituto de Medicina Tropical de São Paulo 2006, 48:151-156.
5. Deborggraeve S., Boelaert M., Rija S., Doncker S., Dujardin J.C., Herdewijn P., Büscher P. Diagnostic accuracy of a new Leishmania PCR for clinical visceral Leishmaniasis in Nepal and its role in diagnosis of disease. Tropical Medicine & International Health 2008, 13:1378-1383.
6. Desjeux P. Leishmaniasis: current situation and new perspectives. Comparative Immunology, Microbiology and Infectious Diseases 2004, 27:305-318.
7. Fernandes A.P., Nelson K., Beverley S.M. Evolution of nuclear ribosomal RNAs in kinetoplastid protozoa: perspectives on the age and origins of parasitism. Proceedings of the National Academy of Sciences 1993, 90:11608-11612.
8. Francino O., Altet L., Sánchez-Robert E., Rodriguez A., Solano-Gallego L., Alberola J., Ferrer L., Sánchez A., Roura X. Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis. Veterinary Parasitology 2006, 137:214-221.
9. Kuhls K., Mauricio I.L., Pratlong F. Analysis of ribosomal DNA internal transcribed spacer sequences of the Leishmania donovani complex. Microbes and Infection 2005, 7:1224-1234.
10. Lainson R., Rangel E.F. Lutzomyia longipalpis and the eco-epidemiology of American visceral Leishmaniases, with particular reference to Brazil - a review. Memórias do Instituto Oswaldo Cruz 2005, 100:811-827.
11. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 2001, 25:402-408.
12. Lukes J., Mauricio I.L., Schönian G., Dujardin J.C., Soteriadou K., Dedet J.P., Kuhls K., Tintaya K.W.Q., Jirku M., Chocholová E., Haralambous C., Pratlong F., Oborník M., Horák A., Ayala F.J., Miles M.A. Evolutionary and geographical history of the Leishmania donovani complex with a revision of current taxonomy. Proceedings of the National Academy of Sciences of the United States of America 2007, 104:9375-9380.
13. Martín-Sánchez J., Gállego M., Barón S., Castillejo S., Morillas-Marquez F. Pool screen PCR for estimating the prevalence of Leishmania infantum infection in sandflies (Diptera: Nematocera, Phlebotomidae). Transactions of the Royal Society of Tropical Medicine and Hygiene 2006, 100:527-532.
14. Mary C., Faraut F., Lascombe L., Dumon H. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. Journal of Clinical Microbiology 2004, 42:5249-5255.
15. Mauricio I.L., Howard M.K., Stothard J.R., Miles M.A. Genetic diversity in the Leishmania donovani complex. Parasitology 1999, 119:237-246.
16. Mauricio I.L., Stothard J.R., Miles M.A. The strange case of Leishmania chagasi. Parasitology Today 2000, 16:188-189.
17. Mortarino M., Franceschi A., Mancianti F., Bazzocchi C., Genchi C., Bandi C. Quantitative PCR in the diagnosis of Leishmania. Parasitologia 2004, 46:163-167.
18. Nicolas L., Prina E., Lang T., Milon G. Real-time PCR for detection and quantification of Leishmania in mouse tissues. Journal of Clinical Microbiology 2002, 40:1666-1669.
19. Oliveira-Pereira Y.N., Rebêlo J.M.M., Moraes J.L.P., Pereira S.R.F. Molecular diagnosis of the natural infection rate due to Leishmania sp in sandflies (Psychodidae, Lutzomyia) in the Amazon region of Maranhão, Brazil. Revista da Sociedade Brasileira de Medicina Tropical 2006, 39:540-543.
20. Paiva B.R., Secundino N.F.C., Nascimento J.C., Pimenta P.F.P., Galati E.A.B., Andrade H.F., Malafronte R.S. Detection and identification of Leishmania species in field-captured phlebotomine sandflies based on mini-exon gene PCR. Acta Tropica 2006, 99:252-259.
21. Paiva B.R., Secundino N.F.C., Pimenta P.F.P., Galati E.A.B., Andrade H.F., Malafronte R.S. Padronização de condições para detecção de DNA de Leishmania spp. em flebotomíneos (Diptera, Psychodidae) pela reação em cadeia da polimerase. Cadernos de Saúde Pública 2007, 23:87-94.
22. Prina E., Roux E., Mattei D., Milon G. Leishmania DNA is rapidly degraded following parasite death: an analysis by microscopy and real-time PCR. Microbes and Infection 2007, 9:1307-1315.
23. Quaresma P.F., Murta S.M.F., Ferreira E.C., Rocha-Lima A.C.V.M., Xavier A.A.P., Gontijo C.M.F. Molecular diagnosis of canine visceral Leishmaniasis: identification of Leishmania species by PCR-RFLP and quantification of parasite DNA by real-time PCR. Acta Tropica 2009, 111:289-294.
24. Ranasinghe S., Rogers M.E., Hamilton J.G.C., Bates P.A., Bates P.A., Maingon R.D.C. A real-time PCR assay to estimate Leishmania chagasi load in its natural sand fly vector Lutzomyia longipalpis. Transactions of the Royal Society of Tropical Medicine and Hygiene 2008, 102:875-882.
25. Rolão N., Cortes S., Rodrigues O.R., Campino L. Quantification of Leishmania infantum parasites in tissue biopsies by real-time polymerase chain reaction and polymerase chain reaction-enzyme-linked immunosorbent assay. Journal of Parasitology 2004, 90:1150-1154.
26. Schulz A., Mellenthin K., Schonian G., Fleischer B., Drosten C. Detection, differentiation, and quantitation of pathogenic Leishmania organisms by a fluorescence resonance energy transfer-based real-time PCR assay. Journal of Clinical Microbiology 2003, 41:1529-1535.
27. Silva E.A., Andreotti R., Dias E.S., Barros J.C., Brazuna J.C.M. Detection of Leishmania DNA in phlebotomines captured in Campo Grande, Mato Grosso do Sul, Brazil. Experimental Parasitology 2008, 119:343-348.
28. Watts P.C., Boyland E., Noyes H.A., Maingon R., Kemp J. Polymorphic dinucleotide microsatellite loci in the sandfly Lutzomyia longipalpis (Diptera: Phlebotominae). Molecular Ecology Notes 2002, 2:62-64.
29. WHO - World Health Organization, 2004. Scientific working group on Leishmaniasis. TDR Research Publication.
30. Young D.G., Duncan M.A. Guide to the identification and geographic distribution of Lutzomyia sand flies in the Mexico the West Indies Central and the South America (Diptera: Psychodidae). Memoirs of the American Entomological Institute 1994, 54. Associate Publishers, Gainesville, pp. 881.