Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM

Methods in Molecular Biology

Volumn 756, Issue , 2011, Pages 325-332

  Yudowski, Guillermo A ^a   von Zastrow, Mark ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Endocytosis; Fluorescence microscopy; Live cell imaging; Receptor; Recycling; Total internal reflection microscopy; Trafficking

Indexed keywords

G PROTEIN COUPLED RECEPTOR;

ARTICLE; CELL LINE; CELL MEMBRANE; ENDOCYTOSIS; FLUORESCENCE MICROSCOPY; HUMAN; METABOLISM; METHODOLOGY; PROTEIN TRANSPORT;

CELL LINE; CELL MEMBRANE; ENDOCYTOSIS; HUMANS; MICROSCOPY, FLUORESCENCE; PROTEIN TRANSPORT; RECEPTORS, G-PROTEIN-COUPLED;

EID: 80054735106     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-160-4_19     Document Type: Chapter

References (17)

1. Sorkin, A., and von Zastrow, M. (2009) Endocytosis and signalling: intertwining molecular networks. Nat Rev Mol Cell Biol 10, 609–22.
2. Shcherbakova, O. G., Hurt, C. M., Xiang, Y., Dell’Acqua, M. L., Zhang, Q., Tsien, R. W., and Kobilka, B. K. (2007) Organization of beta-adrenoceptor signaling compartments by sympathetic innervation of cardiac myocytes. J Cell Biol 176, 521–33.
3. Steyer, J. A., and Almers, W. (2001) A real-time view of life within 100 nm of the plasma membrane. Nat Rev Mol Cell Biol 2, 268–75.
4. Schmoranzer, J., Goulian, M., Axelrod, D., and Simon, S. M. (2000) Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence Microscopy. J Cell Biol 149, 23–32.
5. Goldman, R. D., and Spector. D. L. (2005) Live cell imaging: a laboratory manual. Cold Spring Harbor, N.Y. Cold Spring Harbor Laboratory Press.
6. Lippincott-Schwartz, J., Altan-Bonnet, N., and Patterson, G. H. (2003) Photobleaching and photoactivation: following protein dynamics in living cells. Nat Cell Biol Suppl:S7–14.
7. Miyawaki, A. (2005) Innovations in the imaging of brain functions using fluorescent proteins. Neuron 48, 189–99.
8. Giepmans, B. N., Adams, S. R., Ellisman, M. H., and Tsien, R. Y. (2006) The fluorescent toolbox for assessing protein location and function. Science 312, 217–24.
9. Miesenbock, G., De Angelis, D. A., and Rothman, J. E. (1998) Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394, 192–5.
10. Sankaranarayanan, S., De Angelis, D., Rothman, J. E., and Ryan, T. A. (2000) The use of pHluorins for optical measurements of presynaptic activity. Biophys J 79, 2199–208.
11. Yudowski, G. A., Puthenveedu, M. A., and von Zastrow, M. (2006) Distinct modes of regulated receptor insertion to the somatodendritic plasma membrane. Nat Neurosci 9, 622–7.
12. Waters, J. C. (2009) Accuracy and precision in quantitative fluorescence microscopy. J Cell Biol 185, 1135–48.
13. Bolte, S., and Cordelières, F. P. (2006) A guided tour into subcellular colocalization analysis in light microscopy. J Microsc 224, 213–32.
14. Yudowski, G. A., Puthenveedu, M. A., Leonoudakis, D., Panicker, S., Thorn, K. S., Beattie, E. C., and von Zastrow, M. (2009) Real-time imaging of discrete exocytic events mediating surface delivery of AMPA receptors. J Neurosci 27, 11112–21.
15. Puthenveedu, M. A., and von Zastrow,M. (2006) Cargo regulates clathrin-coated pit dynamics. Cell 127, 113–24.
16. Pucadyil, T. J., and Schmid, S. L. (2008) Real-time visualization of dynamin-catalyzed membrane fission and vesicle release. Cell 135, 1263–75.
17. Bogdanov, A. M., Bogdanova, E. A., Chudakov, D. M., Gorodnicheva, T. V., Lukyanov, S., and Lukyanov, K. A. (2009) Cell culture medium affects GFP photostability: a solution. Nat Methods 6, 859–60.