In Vitro Human Skin Segmentation and Drug Concentration–Skin Depth Profiles

Methods in Molecular Biology

Volumn 763, Issue , 2011, Pages 33-50

  Melero, Ana ^a   Hahn, Tsambika ^a   Schaefer, Ulrich F ^a   Schneider, Marc ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

Cryosectioning; IR D measurements; Skin segmentation; Tape stripping

Indexed keywords

CARBON DIOXIDE;

ARTICLE; BIOPSY; CHEMISTRY; DENSITOMETRY; DIFFUSION; EPIDERMIS; HUMAN; INFRARED RADIATION; KINETICS; METABOLISM; METHODOLOGY; MICROTOMY; ORGAN SIZE; SKIN ABSORPTION; SURGICAL TAPE; ULTRAMICROTOMY; ULTRASTRUCTURE;

BIOPSY; CARBON DIOXIDE; CRYOULTRAMICROTOMY; DENSITOMETRY; DIFFUSION; EPIDERMIS; HUMANS; INFRARED RAYS; KINETICS; MICROTOMY; ORGAN SIZE; SKIN ABSORPTION; SURGICAL TAPE;

EID: 80054733591     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-191-8_2     Document Type: Chapter

References (11)

1. OECD. (2004) Guideline for the Testing of Chemicals. Skin Absorption: in vitro Method, 428.
2. Howes D, Guy R, Hadgraft J, Heylings J, Hoeck U, Kemper F, Maibach H, Marty JP, Merk H, Parra J, Rekkas D, Rondelli I, Schaefer H, Täuber U, Verbiese N. (1996) Methods for Assessing Percutaneous Absorption: The Report and Recommendations of ECVAM Workshop 13. ATLA Alternatives to Laboratory Animals. 24:81–106.
3. REGULATION (EC) No 1907/2006 OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL concerning the Registration E, Authorisation and Restriction of Chemicals (REACH). (2006).
4. Wagner H, Kostka KH, Lehr CM, Schaefer UF. (2000) Drug Distribution in Human Skin Using Two Different In Vitro Test Systems: Comparison with In Vivo Data. Pharmaceutical Research. 17:1475–1481.
5. Hahn T, Hansen S, Neumann D, Kostka KH, Lehr CM, Muys L, Schaefer UF. (2010) Infrared Densitometry: A Fast and Non-Destructive Method for Exact Stratum Corneum Depth Calculation for in vitro Tape-Stripping. Skin Pharmacology and Physiology. 23:183–192.
6. Kalia YN, Pirot F, Guy RH. (1996) Homogeneous Transport in a Heterogeneous Membrane: Water Diffusion Across Human Stratum Corneum In Vivo. Biophysical Journal. 71:2692–2700.
7. Lademann J, Jacobi U, Surber C, Weigmann HJ, Fluhr JW. (2009) The tape stripping procedure – evaluation of some critical parameters. European Journal of Pharmaceutics and Biopharmaceutics. 72:317–323.
8. Dreher F, Modjtahedi BS, Modjtahedi SP, Maibach HI. (2005) Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates. Skin Research and Technology. 11:97–101.
9. Voegeli R, Heiland J, Doppler S, Rawlings AV, Schreier T. (2007) Efficient and simple quantification of stratum corneum proteins on tape strippings by infrared densitometry. Skin Research and Technology. 13:242–251.
10. Harrison SM, Barry BW, Dugard PH. (1984) Effects of freezing on human skin permeability. Journal of Pharmacy and Pharmacology. 36:261–262.
11. Schaefer U. (1996) An ex-vivo model for the study of drug penetration into human skin. Pharmaceutical Research. 13 (Suppl.).