Analysis of Thylakoid Protein Complexes by Two-Dimensional Electrophoretic Systems

Methods in Molecular Biology

Volumn 775, Issue , 2011, Pages 19-30

  Sirpiö, Sari ^a   Suorsa, Marjaana ^a   Aro, Eva Mari ^a  

^a UNIVERSITY OF TURKU   (Finland)

Author keywords

Chloroplast; Electrophoresis; Native gel; Photosynthesis; Protein complex; Thylakoid; Two dimensional gel electrophoresis

Indexed keywords

THYLAKOID MEMBRANE PROTEIN;

ANALYSIS; ARABIDOPSIS; ARTICLE; CHEMISTRY; CYTOLOGY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOLUBILITY; STRUCTURE ACTIVITY RELATION; THYLAKOID; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANALYTIC SAMPLE PREPARATION METHODS; ARABIDOPSIS; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; SOLUBILITY; STRUCTURE-ACTIVITY RELATIONSHIP; THYLAKOID MEMBRANE PROTEINS; THYLAKOIDS;

ARABIDOPSIS THALIANA;

EID: 80054718539     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-237-3_2     Document Type: Chapter

References (4)

1. Kügler, M., Jänsch, L., Kruft, V., Schmitz, U. K., and Braun, H.-P. (1997) Analysis of the chloroplast protein complexes by blue-native polyacrylamide gel electrophoresis (BN-PAGE). Photosynth. Res. 53, 35–44.
2. Wittig, I., Karas, M., and Schägger, H. (2007) High resolution clear native electrophoresis for in-gel functional assays and fluorescence studies of membrane protein complexes. Mol. Cell. Proteomics 7, 1215–1225.
3. Aro, E.M., Suorsa, M., Rokka, A., Allahverdiyeva, Y., Paakkarinen, V., Saleem, A., Battchikova, N., and Rintamäki, E. (2005) Dynamics of photosystem II: A proteomic approach to thylakoid protein complexes. J. Exp. Bot. 56, 347–356.
4. Pesaresi, P., Hertle, A., Pribil, M., Kleine, T., Wagner, R., Strissel, H., Ihnatowicz, A., Bonardi, V., Scharfenberg, M., Schneider, A., Pfannschmidt, T., and Leister, D. (2009) Arabidopsis STN7 kinase provides a link between short-and long-term photo synthetic acclimation. Plant Cell 8, 2402–2423.