Cytomegalovirus DNA stability in EDTA anti-coagulated whole blood and plasma samples

Journal of Clinical Virology

Volumn 52, Issue 3, 2011, Pages 222-224

  Abdul Ali, Deborah ^a,b   Kraft, Colleen S ^a,b   Ingersoll, Jessica ^a,b   Frempong, Mona ^a,b   Caliendo, Angela M ^a,b  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b EMORY CENTER FOR AIDS RESEARCH   (United States)

Author keywords

CMV; Stability; Viral load testing

Indexed keywords

VIRUS DNA;

ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; CYTOMEGALOVIRUS; DNA EXTRACTION; GENE AMPLIFICATION; HUMAN; MOLECULAR STABILITY; NONHUMAN; PLASMA; PRIORITY JOURNAL; TEMPERATURE DEPENDENCE; VIRUS LOAD;

CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; FREEZING; HUMANS; SPECIMEN HANDLING; VIRAL LOAD;

EID: 80054713791     PISSN: 13866532     EISSN: 18735967     Source Type: Journal    
DOI: 10.1016/j.jcv.2011.08.005     Document Type: Article

References (5)

1. Schafer P., Tenschert W., Gutensohn K., Laufs R. Minimal effect of delayed sample processing on results of quantitative PCR for cytomegalovirus DNA in leukocytes compared to results of an antigenemia assay. J Clin Microbiol 1997, 35:741-744.
2. Roberts T.C., Buller R.S., Gaudreault-Keener M., Sternhell K.E., Garlock K., Singer G.G. Effects of storage temperature and time on qualitative and quantitative detection of cytomegalovirus in blood specimens by shell vial culture and PCR. J Clin Microbiol 1997, 35:2224-2228.
3. Schafer P., Tenschert W., Schroter M., Gutensohn K., Laufs R. False-positive results of plasma PCR for cytomegalovirus DNA due to delayed sample preparation. J Clin Microbiol 2000, 38:3249-3253.
4. Boom R., Sol C.J., Schuurman T., Van Breda A., Weel J.F., Beld M. Human cytomegalovirus DNA in plasma and serum specimens of renal transplant recipients is highly fragmented. J Clin Microbiol 2002, 40:4105-4113.
5. Nesbitt S.E., Cook L., Jerome K.R. Cytomegalovirus quantitation by real-time PCR is unaffected by delayed separation of plasma from whole blood. J Clin Microbiol 2004, 42:1296-1297.