Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: Efficiency and cytotoxicity

Journal of Nanobiotechnology

Volumn 9, Issue , 2011, Pages

  Durán, María Carolina ^a   Willenbrock, Saskia ^a   Barchanski, Annette ^b   Müller, Jessika M V ^a   Maiolini, Arianna ^a   Soller, Jan T ^c   Barcikowski, Stephan ^d   Nolte, Ingo ^a   Feige, Karsten ^a   Murua Escobar, Hugo ^a  

^a UNIVERSITY OF VETERINARY MEDICINE HANNOVER   (Germany)

^b LASER ZENTRUM HANNOVER E V   (Germany)

^c UNIVERSITY OF GÖTTINGEN   (Germany)

^d UNIVERSITY OF DUISBURG ESSEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CONVENTIONAL APPROACH; CONVENTIONAL METHODS; CYTOTOXIC; CYTOTOXIC EFFECTS; DIFFERENT SIZES; DNA EXPRESSIONS; LIGAND-FREE; LIGAND-STABILIZED; MAMMALIAN CELL LINES; NANOPARTICLE FORMULATION; PROPIDIUM IODIDE; RECOMBINANT PROTEIN; SIDE EFFECT; STABILIZER-FREE; TRANSFECTION EFFICIENCY; TRANSFECTION METHODS; TRANSFECTION PROTOCOL; TRANSFECTION REAGENTS;

CELL CULTURE; CELL PROLIFERATION; CELLS; CYTOTOXICITY; DNA; EFFICIENCY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; LIGANDS; MAGNETS; MAMMALS; NANOPARTICLES; PROTEINS; TOXICITY;

MOLECULAR BIOLOGY;

NANOPARTICLE; PLASMID DNA; PROPIDIUM IODIDE; DNA; GOLD; GREEN FLUORESCENT PROTEIN; METAL NANOPARTICLE;

ANIMAL CELL; ARTICLE; CELL PROLIFERATION; COMPARATIVE STUDY; CYTOTOXICITY; DNA TRANSFECTION; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; IMMUNOFLUORESCENCE; MAMMAL CELL; NONHUMAN; PROTEIN EXPRESSION; CELL LINE; CELL SURVIVAL; CHEMISTRY; DRUG EFFECT; GENETIC TRANSFECTION; GENETICS; HUMAN; LASER; METABOLISM; PLASMID;

EUKARYOTA; MAMMALIA;

CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; DNA; FLOW CYTOMETRY; GOLD; GREEN FLUORESCENT PROTEINS; HUMANS; LASERS; METAL NANOPARTICLES; MICROSCOPY, FLUORESCENCE; PLASMIDS; TRANSFECTION;

EID: 80054710886     PISSN: None     EISSN: 14773155     Source Type: Journal    
DOI: 10.1186/1477-3155-9-47     Document Type: Article

References (22)

1. Baumgart J, Bintig W, Ngezahayo A, Willenbrock S, Murua Escobar H, Ertmer W, Lubatschowski H, Heisterkamp A. Quantified femtosecond laser based opto-perforation of living GFSHR-17 and MTH53 a cells. Opt Express 2008, 16:3021-3031. 10.1364/OE.16.003021, 18542388.
2. Petersen S, Soller JT, Wagner S, Richter A, Bullerdiek J, Nolte I, Barcikowski S, Murua Escobar H. Co-transfection of plasmid DNA and laser-generated gold nanoparticles does not disturb the bioactivity of GFP-HMGB1 fusion protein. J Nanobiotechnology 2009, 7:6. 10.1186/1477-3155-7-6, 2775017, 19852831.
3. Bertram J. MATra - Magnet Assisted Transfection: combining nanotechnology and magnetic forces to improve intracellular delivery of nucleic acids. Curr Pharm Biotechnol 2006, 7:277-285. 10.2174/138920106777950825, 16918404.
4. Ghosh P, Han G, De M, Kim CK, Rotello VM. Gold nanoparticles in delivery applications. Adv Drug Deliv Rev 2008, 60:1307-1315. 10.1016/j.addr.2008.03.016, 18555555.
5. Rosi NL, Giljohann DA, Thaxton CS, Lytton-Jean AK, Han MS, Mirkin CA. Oligonucleotide-modified gold nanoparticles for intracellular gene regulation. Science 2006, 312:1027-1030. 10.1126/science.1125559, 16709779.
6. Borm PJ, Robbins D, Haubold S, Kuhlbusch T, Fissan H, Donaldson K, Schins R, Stone V, Kreyling W, Lademann J, Krutmann J, Warheit D, Oberdorster E. The potential risks of nanomaterials: a review carried out for ECETOC. Part Fibre Toxicol 2006, 3:11. 10.1186/1743-8977-3-11, 1584248, 16907977.
7. Dahl JA, Maddux BL, Hutchison JE. Toward greener nanosynthesis. Chem Rev 2007, 107:2228-2269. 10.1021/cr050943k, 17564480.
8. Sylvestre J, Poulin S, Kabashin AV, Sacher E, Meunier M, Luong JHT. Surface Chemistry of Gold Nanoparticles Produced by Laser Ablation in Aqueous Media. J Phys Chem B 2004, 108:16864-16869.
9. Petersen S, Barcikowski S. In Situ Bioconjugation: Single Step Approach to Tailored Nanoparticle-Bioconjugates by Ultrashort Pulsed Laser Ablation. Adv Funct Mater 2009, 19:1167-1172.
10. Luo D, Saltzman WM. Enhancement of transfection by physical concentration of DNA at the cell surface. Nat Biotechnol 2000, 18:893-895. 10.1038/78523, 10932162.
11. Kamau SW, Hassa PO, Steitz B, Petri-Fink A, Hofmann H, Hofmann-Amtenbrink M, von Rechenberg B, Hottiger MO. Enhancement of the efficiency of non-viral gene delivery by application of pulsed magnetic field. Nucleic Acids Res 2006, 34:e40. 10.1093/nar/gkl035, 1408310, 16540591.
12. Schakowski F, Gorschluter M, Junghans C, Schroff M, Buttgereit P, Ziske C, Schottker B, Konig-Merediz SA, Sauerbruch T, Wittig B, Schmidt-Wolf IG. A novel minimal-size vector (MIDGE) improves transgene expression in colon carcinoma cells and avoids transfection of undesired DNA. Mol Ther 2001, 3:793-800. 10.1006/mthe.2001.0322, 11356084.
13. Chithrani BD, Ghazani AA, Chan WC. Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Lett 2006, 6:662-668. 10.1021/nl052396o, 16608261.
14. Yin W, Xiang P, Li Q. Investigations of the effect of DNA size in transient transfection assay using dual luciferase system. Anal Biochem 2005, 346:289-294. 10.1016/j.ab.2005.08.029, 16213455.
15. Renker B, Diestel S, Ruonala M, Bertram J. MATra ein Trojanisches Pferd fuer eine zellschonende Transfektion. BIOspektrum 2010, 1-2.
16. Pan Y, Neuss S, Leifert A, Fischler M, Wen F, Simon U, Schmid G, Brandau W, Jahnen-Dechent W. Size-dependent cytotoxicity of gold nanoparticles. Small 2007, 3:1941-1949. 10.1002/smll.200700378, 17963284.
17. Sperling RA, Rivera Gil P, Zhang F, Zanella M, Parak WJ. Biological applications of gold nanoparticles. Chem Soc Rev 2008, 37:1896-1908. 10.1039/b712170a, 18762838.
18. Connor EE, Mwamuka J, Gole A, Murphy CJ, Wyatt MD. Gold nanoparticles are taken up by human cells but do not cause acute cytotoxicity. Small 2005, 1:325-327. 10.1002/smll.200400093, 17193451.
19. Taylor U, Klein S, Petersen S, Kues W, Barcikowski S, Rath D. Nonendosomal cellular uptake of ligand-free, positively charged gold nanoparticles. Cytometry A 77:439-446.
20. Shukla R, Bansal V, Chaudhary M, Basu A, Bhonde RR, Sastry M. Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview. Langmuir 2005, 21:10644-10654. 10.1021/la0513712, 16262332.
21. Pernodet N, Fang X, Sun Y, Bakhtina A, Ramakrishnan A, Sokolov J, Ulman A, Rafailovich M. Adverse effects of citrate/gold nanoparticles on human dermal fibroblasts. Small 2006, 2:766-773. 10.1002/smll.200500492, 17193121.
22. Brandenberger C, Rothen-Rutishauser B, Muhlfeld C, Schmid O, Ferron GA, Maier KL, Gehr P, Lenz AG. Effects and uptake of gold nanoparticles deposited at the air-liquid interface of a human epithelial airway model. Toxicol Appl Pharmacol 242:56-65.