Relationship between rumen methanogens and methane production in dairy cows fed diets supplemented with a feed enzyme additive

Journal of Applied Microbiology

Volumn 111, Issue 5, 2011, Pages 1148-1158

  Zhou, M ^a   Chung, Y H ^b   Beauchemin, K A ^b   Holtshausen, L ^b   Oba, M ^a   McAllister, T A ^b   Guan, L L ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

^b LETHBRIDGE RESEARCH CENTRE   (Canada)

Author keywords

Dairy; Denaturing gradient gel electrophoresis; Methane; Methanogens; Rumen

Indexed keywords

ECOLOGY; ELECTROPHORESIS; ENZYMES; METHANOGENS; POLYMERASE CHAIN REACTION;

CH 4; DAIRY COW; DENATURING GRADIENT GEL ELECTROPHORESIS; GEL ELECTROPHORESIS ANALYSIS; LACTATING DAIRY COWS; METHANE PRODUCTION; METHANOGENIC ARCHAEA; REAL-TIME PCR; RUMEN; SAMPLING TIME;

METHANE;

FOOD ADDITIVE; METHANE;

CATTLE; DIET; ELECTROKINESIS; ENZYME ACTIVITY; FOOD SUPPLEMENTATION; HYDROXYL RADICAL; METHANE; METHANOGENIC BACTERIUM; MICROBIAL COMMUNITY; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME;

ARTICLE; BACTERIAL STRAIN; COW; DAIRY CATTLE; DENATURING GRADIENT GEL ELECTROPHORESIS; DIETARY INTAKE; ECOLOGY; METHANOBREVIBACTER SMITHII; METHANOGEN; MICROBIAL COMMUNITY; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RUMINANT STOMACH;

ARCHAEA; METHANOBREVIBACTER GOTTSCHALKII; METHANOBREVIBACTER SMITHII;

EID: 80054680001     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2011.05126.x     Document Type: Article

References (48)

1. Arriola, K.G., Kim, S.C., Staples, C.R. and Adesogan, A.T. (2011) Effect of fibrolytic enzyme application to low- and high-concentrate diets on the performance of lactating dairy cattle. J Dairy Sci 94, 832-841.
2. Bano, N., Ruffin, S., Ransom, B. and Hollibaugh, J.T. (2004) Phylogenetic composition of Arctic Ocean archaeal assemblages and comparison with Antarctic assemblages. Appl Environ Microbiol 70, 781-789.
3. Beauchemin, K.A., Colombatto, D., Morgavi, D.P. and Yang, W.Z. (2003) Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. J Anim Sci 81, E37-E47.
4. Dawson, K.A. and Tricarico, J.M. (2007) The use of exogenous fibrolytic enzymes to enhance microbial activities in the rumen and the performance of ruminant animals. Available at: (accessed 20 March 2011).
5. Guan, L.L., Nkrumah, J.D., Basarab, J.A. and Moore, S.S. (2008) Linkage of microbial ecology to phenotype: correlation of rumen microbial ecology to cattle's feed efficiency. FEMS Microbiol Lett 288, 85-91.
6. Gupta, A. and Chaudhary, U.B. (2009) Available at: (accessed 20 March 2011).
7. Holtshausen, L., Chung, Y.-H., Gerardo-Cuervo, H., Oba, M. and Beauchemin, K.A. (2011) Improved milk production efficiency in early lactation dairy cattle with dietary addition of a developmental fibrolytic enzyme additive. J Dairy Sci 94, 899-907.
8. Hook, S.E., Northwood, K.S., Wright, A.D. and McBride, B.W. (2009) Long-term monensin supplementation does not significantly affect the density or diversity of methanogens in the rumen of the lactating dairy cow. Appl Environ Microbiol 75, 374-380.
9. Hook, S.E., Steele, M.A., Northwood, K.S., Wright, A.D. and McBride, B.W. (2011) Impact of high-concentrate feeding and low ruminal pH on methanogens and protozoa in the rumen of dairy cows. Microb Ecol 62, 94-105.
10. Janssen, P.H. and Kris, M. (2008) Structure of the archael community of the rumen. Appl Environ Microbiol 74, 3619-3625.
11. Jarvis, G.N., Strompl, C., Burgess, D.M., Skillman, L.C., Moore, E.R. and Joblin, K.N. (2000) Isolation and identification of ruminal methanogens from grazing cattle. Curr Microbiol 40, 327-332.
12. Johnson, K.A. and Johnson, D.E. (1995) Methane emissions from cattle. J Anim Sci 73, 2483-2492.
13. Johnson, D.E. and Ward, G.M. (1996) Estimates of animal methane emissions. Environ Monit Assess 42, 133-141.
14. Johnson, K.A., Huyler, M., Westberg, H., Lamb, B. and Zimmerman, P. (1994) Measurement of methane emissions from ruminant livestock using a sulfur hexafluoride tracer technique. Environ Sci Technol 28, 359-362.
15. Klappenbach, J.A., Saxman, P.R., Cole, J.R. and Schmidt, T.M. (2001) rrndb: the ribosomal RNA operon copy number database. Nucleic Acids Res 29, 181-184.
16. Leahy, S.C., Kelly, W.J., Altermann, E., Ronimus, R.S., Yeoman, C.J., Pacheco, D.M., Li, D., Kong, Z. et al. (2010) The genome sequence of the rumen methanogen Methanobrevibacter ruminantium reveals new possibilities for controlling ruminant methane emissions. PLoS ONE 5, e8926.
17. Leedle, J.A. and Greening, R.C. (1988) Postprandial changes in methanogenic and acidogenic bacteria in the rumens of steers fed high- or low-forage diets once daily. Appl Environ Microbiol 54, 502-506.
18. Lewis, G.E., Sanchez, W.K., Hunt, C.W., Guy, M.A., Pritchard, G.T., Swanson, B.I. and Treacher, R.J. (1999) Effect of direct-fed fibrolytic enzymes on the lactational performance of dairy cows. J Dairy Sci 82, 611-617.
19. Martin, C., Morgavi, D.P. and Doreau, M. (2010) Methane mitigation in ruminants: from microbe to the farm scale. Animal 4, 351-365.
20. Martinez, E.D., Klieve, A.V., Ouwerkerk, D., Swain, T., Gulino, L.M., Turnbull, K. and Poppi, D.P. (2010) Between animal variance in ruminal bacteria and protozoal communities from DGGE profiles of steers on a low quality forage diet. Proc Aust Soc Anim Prod 28, 63.
21. McAllister, T.A., Okine, E.K., Mathison, G.W. and Cheng, K.J. (1996) Dietary, environmental and microbiological aspects of methane production in ruminants. Can J Anim Sci 76, 231-243.
22. McGinn, S.M., Chung, Y.-H., Beauchemin, K.A., Iwaasa, A.D. and Grainger, C. (2009) Use of corn distillers' dried grains to reduce enteric methane loss from beef cattle. Can J Anim Sci 89, 409-413.
23. McMichael, A.J., Powles, J.W., Butler, C.D. and Uauy, R. (2007) Food, livestock production, energy, climate change, and health. Lancet 370, 1253-1263.
24. Miller, T.L. and Lin, C. (2002) Description of Methanobrevibacter gottschalkii sp. nov., Methanobrevibacter thaueri sp. nov., Methanobrevibacter woesei sp. nov. and Methanobrevibacter wolinii sp. nov. Int J Syst Evol Microbiol 52, 819-822.
25. Miller, T.L., Wolin, M.J., Zhao, H.X. and Bryant, M.P. (1986) Characteristics of methanogens isolated from bovine rumen. Appl Environ Microbiol 51, 201-202.
26. 2-utilizing acetogenic and sulfate-reducing bacteria and methanogenic archaea from digestive tract of different mammals. Curr Microbiol 32, 129-133.
27. Oppermann, R.A., Nelson, W.O. and Brown, R.E. (1957) In vitro studies on methanogenic rumen bacteria. J Dairy Sci 40, 770-788.
28. Paynter, M.J. and Hungate, R.E. (1968) Characterization of Methanobacterium mobilis, sp. n., isolated from the bovine rumen. J Bacteriol 95, 1943-1951.
29. Rea, S., Bowman, J.P., Popovski, S., Pimm, C. and Wright, A.D. (2007) Methanobrevibacter millerae sp. nov. and Methanobrevibacter olleyae sp. nov., methanogens from the ovine and bovine rumen that can utilize formate for growth. Int J Syst Evol Microbiol 57, 450-456.
30. Rode, L.M., Yang, W.Z. and Beauchemin, K.A. (1999) Fibrolytic enzyme supplements for dairy cows in early lactation. J Dairy Sci 82, 2121-2126.
31. Saengkerdsub, S., Anderson, R.C., Wilkinson, H.H., Kim, W.K., Nisbet, D.J. and Ricke, S.C. (2007) Identification and quantification of methanogenic Archaea in adult chicken ceca. Appl Environ Microbiol 73, 353-356.
32. Samuel, B.S., Hansen, E.E., Manchester, J.K., Coutinho, P.M., Henrissat, B., Fulton, R., Latreille, P., Kim, K. et al. (2007) Genomic and metabolic adaptations of Methanobrevibacter smithii to the human gut. Proc Natl Acad Sci USA 104, 10643-10648.
33. Skillman, L.C., Evans, P.N., Strompl, C. and Joblin, K.N. (2006) 16S rDNA directed PCR primers and detection of methanogens in the bovine rumen. Lett Appl Microbiol 42, 222-228.
34. Tajima, K., Nagamine, T., Matsui, H., Nakamura, M. and Aminov, R.I. (2001) Phylogenetic analysis of archaeal 16S rRNA libraries from the rumen suggests the existence of a novel group of archaea not associated with known methanogens. FEMS Microbiol Lett 200, 67-72.
35. Tatsuoka, N., Mohammed, N., Mitsumori, M., Hara, K., Kurihara, M. and Itabashi, H. (2004) Phylogenetic analysis of methyl coenzyme-M reductase detected from the bovine rumen. Lett Appl Microbiol 39, 257-260.
36. Tokura, M., Chagan, I., Ushida, K. and Kojima, Y. (1999) Phylogenetic study of methanogens associated with rumen ciliates. Curr Microbiol 39, 123-128.
37. Ushida, K., Tokura, M., Takenaka, A. and Itabashi, H. (1997) Ciliate protozoa and ruminal methanogenesis. In Rumen Microbes and Digestive Physiology in Ruminants ed. Onodera, R., Itabashi, H., Yano, H. and Sasaki, Y. pp. 209-220. Tokyo: Japan Scientific Society Press.
38. Van Nevel, C.J. and Demeyer, D.I. (1977) Effect of monensin on rumen metabolism in vitro. Appl Environ Microbiol 34, 251-257.
39. Watanabe, T., Asakawa, S., Nakamura, A., Nagaoka, K. and Kimura, M. (2004) DGGE method for analyzing 16S rDNA of methanogenic archaeal community in paddy field soil. FEMS Microbiol Lett 232, 153-163.
40. Weimer, P.J., Waghorn, G.C., Odt, C.L. and Mertens, D.R. (1999) Effect of diet on populations of three species of ruminal cellulolytic bacteria in lactating dairy cows. J Dairy Sci 82, 122.
41. Whitford, M.F., Teather, R.M. and Forster, R.J. (2001) Phylogenetic analysis of methanogens from the bovine rumen. BMC Microbiol 1, 5.
42. Wright, A.D. and Pimm, C. (2003) Improved strategy for presumptive identification of methanogens using 16S riboprinting. J Microbiol Methods 55, 337-349.
43. Wright, A.D., Williams, A.J., Winder, B., Christophersen, C.T., Rodgers, S.L. and Smith, K.D. (2004) Molecular diversity of rumen methanogens from sheep in Western Australia. Appl Environ Microbiol 70, 1263-1270.
44. Wright, A.D., Auckland, C.H. and Lynn, D.H. (2007) Molecular diversity of methanogens in feedlot cattle from Ontario and Prince Edward Island, Canada. Appl Environ Microbiol 73, 4206-4210.
45. Yang, W.Z., Beauchemin, K.A. and Rode, L.M. (2000) A comparison of methods of adding fibrolytic enzymes to lactating cow diets. J Dairy Sci 83, 2512-2520.
46. Yu, Z., Garcia-Gonzalez, R., Schanbacher, F.L. and Morrison, M. (2008) Evaluations of different hypervariable regions of archaeal 16S rRNA genes in profiling of methanogens by Archaea-specific PCR and denaturing gradient gel electrophoresis. Appl Environ Microbiol 74, 889-893.
47. Zhou, M., Hernandez-Sanabria, E. and Guan le, L. (2009) Assessment of the microbial ecology of ruminal methanogens in cattle with different feed efficiencies. Appl Environ Microbiol 75, 6524-6533.
48. Zhou, M., Hernandez-Sanabria, E. and Guan le, L. (2010) Characterization of variation in rumen methanogenic communities under different dietary and host feed efficiency conditions, as determined by PCR-denaturing gradient gel electrophoresis analysis. Appl Environ Microbiol 76, 3776-3786.