Using Vaccinia's innate ability to introduce DNA into mammalian cells for production of recombinant proteins

Journal of Biotechnology

Volumn 156, Issue 3, 2011, Pages 211-213

  Jester, Brian C ^a   Drillien, Robert ^a   Ruff, Marc ^a   Florentz, Catherine ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

Author keywords

Infection; MVA; Recombinant; T7; Transfection; Vaccinia

Indexed keywords

INFECTION; MVA; RECOMBINANT; T7; TRANSFECTION; VACCINIA;

MAMMALS; VIRUSES;

PROTEINS;

PLASMID DNA; RECOMBINANT PROTEIN;

ARTICLE; INNATE IMMUNITY; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; VACCINIA VIRUS;

ANIMALS; BIOTECHNOLOGY; DNA; PLASMIDS; RECOMBINANT PROTEINS; TRANSFECTION; VACCINIA; VACCINIA VIRUS; VIRUS INTEGRATION;

EUKARYOTA; MAMMALIA; VACCINIA VIRUS;

EID: 80054102219     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.09.013     Document Type: Article

References (9)

1. Fuerst T.R., Niles E.G., Studier F.W., Moss B. Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc. Natl. Acad. Sci. U.S.A. 1986, 83:8122-8126.
2. Hebben M., Brants J., Birck C., Samama J.P., Wasylyk B., Spehner D., Pradeau K., Domi A., Moss B., Schultz P., Drillien R. High level protein expression in mammalian cells using a safe viral vector: modified vaccinia virus Ankara. Protein Exp. Purif. 2007, 56:269-278.
3. Lorenzo M.M., Galindo I., Blasco R. Construction and isolation of recombinant vaccinia virus using genetic markers. Methods Mol. Biol. 2004, 269:15-30.
4. Wyatt L.S., Earl P.L., Xiao W., Americo J.L., Cotter C.A., Vogt J., Moss B. Elucidating and minimizing the loss by recombinant vaccinia virus of human immunodeficiency virus gene expression resulting from spontaneous mutations and positive selection. J. Virol. 2009, 83:7176-7184.
5. Mercer J., Helenius A. Vaccinia virus uses macropinocytosis and apoptotic mimicry to enter host cells. Science 2008, 320:531-535.
6. Mercer J., Knébel S., Schmidt F.I., Crouse J., Burkard C., Helenius A. Vaccinia virus strains use distinct forms of macropinocytosis for host-cell entry. Proc. Natl. Acad. Sci. U.S.A. 2010, 107:9346-9351.
7. Laliberte J.P., Moss B. Appraising the apoptotic mimicry model and the role of phospholipids for poxvirus entry. Proc. Natl. Acad. Sci. U.S.A. 2009, 106:17517-17521.
8. Earl P.L., Moss B. Characterization of recombinant vaccinia viruses and their products. Curr. Protoc. Mol. Biol. 1998, UNIT 16.18.
9. Pradeau-Aubreton K., Ruff M., Garnier J.M., Schultz P., Drillien R. Vectors for recombinational cloning and gene expression in mammalian cells using modified vaccinia virus Ankara. Anal. Biochem. 2010, 404:103-105.