Purification of Human PARP-1 and PARP-1 Domains from Escherichia coli for Structural and Biochemical Analysis

Methods in Molecular Biology

Volumn 780, Issue , 2011, Pages 209-226

  Langelier, Marie France ^a   Planck, Jamie L ^a   Servent, Kristin M ^a   Pascal, John M ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

Automodification assays; PARP 1 domains; Protein expression; Protein purification; Structural studies; X ray crystallography

Indexed keywords

NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; PARP1 PROTEIN, HUMAN;

ARTICLE; CHEMISTRY; ESCHERICHIA COLI; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; X RAY CRYSTALLOGRAPHY;

CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; HUMANS; POLY(ADP-RIBOSE) POLYMERASES;

ESCHERICHIA COLI;

EID: 80054075321     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-270-0_13     Document Type: Chapter

References (8)

1. D’Amours D, Desnoyers S, D’Silva I, Poirier GG (1999) Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions. Biochem J 342(Pt 2):249–268
2. Gradwohl G, Menissier de Murcia JM, Molinete M, Simonin F, Koken M, Hoeijmakers JH, de Murcia G (1990) The second zinc-finger domain of poly(ADP-ribose) polymerase determines specificity for single-stranded breaks in DNA. Proc Natl Acad Sci USA 87:2990–2994
3. Ikejima M, Noguchi S, Yamashita R, Ogura T, Sugimura T, Gill DM, Miwa M (1990) The zinc fingers of human poly(ADP-ribose) polymerase are differentially required for the recognition of DNA breaks and nicks and the consequent enzyme activation. Other structures recognize intact DNA. J Biol Chem 265:21907–21913
4. Langelier MF, Servent KM, Rogers EE, Pascal JM (2008) A third zinc-binding domain of human poly(ADP-ribose) polymerase-1 coordi- nates DNA-dependent enzyme activation. J Biol Chem 283:4105–4114
5. Langelier MF, Ruhl DD, Planck JL, Kraus WL, Pascal JM (2010) The Zn3 domain of human poly(ADP-ribose) polymerase-1 (PARP-1) functions in both DNA-dependent poly(ADP-ribose) synthesis activity and chromatin compaction. J Biol Chem 285:18877–18887
6. Tao Z, Gao P, Hoffman DW, Liu HW (2008) Domain C of human poly(ADP-ribose) polymerase-1 is important for enzyme activity and contains a novel zinc-ribbon motif. Biochemistry 47:5804–5813
7. Altmeyer M, Messner S, Hassa PO, Fey M, Hottiger MO (2009) Molecular mechanism of poly(ADP-ribosyl)ation by PARP1 and identification of lysine residues as ADP-ribose acceptor sites. Nucleic Acids Res 37:3723–3738
8. Tao Z, Gao P, Liu HW (2009) Identification of the ADP-ribosylation sites in the PARP-1 automodification domain: analysis and implications. J Am Chem Soc 131:14258–14260