Synthesis and performance of 3D-Megaporous structures for enzyme immobilization and protein capture

Biotechnology Progress

Volumn 27, Issue 5, 2011, Pages 1329-1338

  Bibi, Noor Shad ^a,b   Gavara, Poondi Rajesh ^a   Espinosa, Silvia L Soto ^c   Grasselli, Mariano ^c   Fernández Lahore, Marcelo ^a  

^a JACOBS UNIVERSITY BREMEN   (Germany)

^b KOHAT UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Pakistan)

^c UNIVERSIDAD NACIONAL DE QUILMES   (Argentina)

Author keywords

Biocatalysis; Bioseparation; Chromatography; Direct capture; Enzyme immobilization; Megaporous structures

Indexed keywords

BINDING CAPACITIES; BIO-SEPARATION; BIOCATALYSIS; BOVINE SERUM ALBUMINS; CHEMICAL SYNTHESIS; CROSSLINKER; DIRECT CAPTURE; DYNAMIC BINDING CAPACITY; FLOWTHROUGH; HEN EGG WHITE LYSOZYME; IONIC CAPACITY; MICROBIAL CELLS; MODEL PROTEINS; PARTICLE EMBEDDING; POTENTIAL APPLICATIONS; PROTEIN BINDING; RADIATION-INDUCED GRAFTING; RESIDENCE TIME; ROBUST SCAFFOLD;

BIOCHEMISTRY; CHROMATOGRAPHY; CLARIFICATION; ENZYME IMMOBILIZATION; GRAFTING (CHEMICAL); ION EXCHANGE; PROTEINS; SUSPENSIONS (FLUIDS); THREE DIMENSIONAL;

BIOSYNTHESIS;

BOVINE SERUM ALBUMIN; IMMOBILIZED ENZYME; LYSOZYME;

ARTICLE; BIOCATALYSIS; CHEMISTRY; LIQUID CHROMATOGRAPHY; PROTEIN BINDING; PROTEIN CONFORMATION; SCANNING ELECTRON MICROSCOPY;

BIOCATALYSIS; CHROMATOGRAPHY, LIQUID; ENZYMES, IMMOBILIZED; MICROSCOPY, ELECTRON, SCANNING; MURAMIDASE; PROTEIN BINDING; PROTEIN CONFORMATION; SERUM ALBUMIN, BOVINE;

BOVINAE; MICROCOCCUS;

EID: 80053925380     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.648     Document Type: Article

References (38)

1. Dainiak MB, Kumar A, Plieva FM, Galaev IY, Mattiasson B. Integrated isolation of antibody fragments from microbial cell culture fluids using supermacroporous cryogels. J Chromatogr A. 2004; 1045: 93-98.
2. Jungbauer A. Chromatographic media for bioseparation. J Chromatogr A. 2005; 1065: 3-12.
3. Braas G, Searle PF, Slater NK, Lyddiatt A. Strategies for the isolation and purification of retroviral vectors for gene therapy. Bioseparation. 1996; 6: 211-228.
4. Lowe CR, Lowe AR, Gupta G. New developments in affinity chromatography with potential application in the production of biopharmaceuticals. J Biochem Biophys Methods. 2001; 49: 561-574.
5. Fernandez-Lahore HM, Kleef R, Kula M, Thömmes J. The influence of complex biological feedstock on the fluidization and bed stability in expanded bed adsorption. Biotechnol Bioeng. 1999; 64: 484-496.
6. Hjorth R. Expanded-bed adsorption in industrial bioprocessing: recent developments. Trends Biotechnol. 1997; 15: 230-235.
7. Bermejo R, Ruiz E, Acien FG. Recovery of B-phycoerythrin using expanded bed adsorption chromatography: scale-up of the process. Enzyme Microb Technol. 2007; 40: 927-933.
8. Osório NM, Gusmão JH, da Fonseca MM, Ferreira-Dias S. Lipase-catalysed interesterification of palm stearin with soybean oil in a continuous fluidised-bed reactor. Eur J Lipid Sci Tech. 2005; 107: 455-463.
9. Berensmeier S, Ergezinger M, Bohnet M, Buchholz K. Design of immobilised dextransucrase for fluidised bed application. J Biotechnol. 2004; 114: 255-267.
10. Hedstrom M, Plieva FM, Galaev IY, Mattiasson B. Monolithic macroporous albumin/chitosan cryogel structure: a new matrix for enzyme immobilization Anal Bioanal Chem. 2008; 390: 907-912.
11. Lozinsky VI, Plieva FM. Poly(vinyl alcohol) cryogels employed as matrices for cell immobilization. 3. Overview of recent research and developments. Enzyme Microb Technol. 1998; 23: 227-242.
12. Lozinsky VI. Cryogels on the basis of natural and synthetic polymers: preparation, properties and application. Russ Chem Rev. 2002; 71: 489-511.
13. Yao K, Yun J, Shen S, Wang L, He X, Yu X. Characterization of a novel continuous supermacroporous monolithic cryogel embedded with nanoparticles for protein chromatography. J Chromatogr A. 2006; 1109: 103-110.
14. Yao K, Yun J, Shen S, Chen F. In-situ graft-polymerization preparation of cation-exchange supermacroporous cryogel with sulfo groups in glass columns. J Chromatogr A. 2007; 1157: 246-251.
15. Grasselli M, Navarro del Cañizo AA, Camperi SA, Wolman FJ, Smolko EE, Cascone O. Immobilized metal ion affinity hollow-fibre membranes obtained by the direct grafting technique. Radiat Phys Chem. 1999; 55: 203-208.
16. Plieva FM, Savina IN, Deraz S, Andersson J, Galaev IY, Mattiasson B. Characterization of supermacroporous monolithic polyacrylamide based matrices designed for chromatography of bioparticles. J Chromatogr B. 2004; 807: 129-137.
17. Lendero N, Vidic J, Brne P, Frankovic V, Strancar A, Podgornik A. Characterization of ion exchange stationary phases via pH transition profiles. J Chromatogr A. 2008; 1185: 59-70.
18. Galyean RD, Cotterill OJ. Ion-exchange chromatographic determination of lysozyme in egg white. J Food Sci. 1981; 46: 1827-1834.
19. Chen Z, Xu L, Liang Y, Wang J, Zhao M, Li Y. Polyethylene glycol diacrylate-based supermacroporous monolithic cryogel as high-performance liquid chromatography stationary phase for protein and polymeric nanoparticle separation. J Chromatogr A. 2008; 1182: 128-131.
20. Plieva FM, Karlsson M, Aguilar M, Gomez D, Mikhalovsky S, Galaev IY. Pore structure in supermacroporous polyacrylamide based cryogels. Soft Matter. 2005; 1: 303-309.
21. Zhang M, Sun Y. Poly(glycidyl methacrylate-divinylbenzene-triallylisocyanurate) continuous-bed protein chromatography. J Chromatogr A. 2001; 912: 31-38.
22. Ceylan D, Ozmen MM, Okay O. Swelling-deswelling kinetics of ionic poly(acrylamide) hydrogels and cryogels. J Appl Polym Sci. 2006; 99: 7.
23. Xian-Zheng Z, Chih-Chang C. Synthesis of temperature sensitive PNIPAAm cryogels in organic solvent with improved properties. J Mater Chem. 2003; 13: 2457-2464.
24. Patrik P, Oxana B, Fatima P, Igor YG, Bo M, Bernt N, Anders A. Characterization of a continuous supermacroporous monolithic matrix for chromatographic separation of large bioparticles. Biotechnol Bioeng. 2004; 88: 224-236.
25. Savina IN, Mattiasson B, Galaev IY. Graft polymerization of acrylic acid onto macroporous polyacrylamide gel (cryogel) initiated by potassium diperiodatocuprate. Polymer. 2005; 46: 9596-9603.
26. Chen F, Yao K, Shen S, Yun J. Influence of grafting conditions on the properties of polyacrylamide-based cation-exchange cryogels grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid. Chem Eng Sci. 2008; 63: 71-77.
27. Savina IN, Galaev IY, Mattiasson B. Anion-exchange supermacroporous monolithic matrices with grafted polymer brushes of N, N-dimethylaminoethyl-methacrylate. J Chromatogr A. 2005; 1092: 199-205.
28. Savina IN, Mattiasson B, Galaev IY. Graft polymerization of acrylic acid onto macroporous polyacrylamide gel (cryogel) initiated by potassium diperiodatocuprate. Polymer. 2005; 46: 9596-9603.
29. Billakanti JM, Fee CJ. Characterization of cryogel monoliths for extraction of minor proteins from milk by cation exchange. Biotechnol Bioeng. 2009; 103: 1155-1163.
30. Bereli N, Andaç M, Baydemir G, Say R, Galaev IY, Denizli A. Protein recognition via ion-coordinated molecularly imprinted supermacroporous cryogels. J Chromatogr A. 2008; 1190: 18-26.
31. Okamura D, Saito K, Sugita K, Tamada M, Sugo T. Solvent effect on protein binding by polymer brush grafted onto porous membranes. J Chromatogr A. 2002; 953: 101-109.
32. Wolman FJ, Smolko EE, Cascone O, Grasselli M. Improved hollow-fibre membranes for dye-affinity chromatography. J Sep Sci. 2005; 28: 45-51.
33. Ventura AM, Lahore HMF, Smolko EE, Grasselli M. High-speed protein purification by adsorptive cation-exchange hollow-fiber cartridges. J Membr Sci. 2008; 321: 350-355.
34. Carbajal ML, Espinoza SLS, Valdez SN, Poskus E, Smolko EE, Grasselli M. Arsenical grafted membranes for immobilization of thioredoxin-like proteins. React Funct Polym. 2009; 69: 816-820.
35. Camperi SA, Grasselli M, Smolko EE, Cascone O. Preparation and characterisation of immobilised metal ion hollow-fibre polysulphone membranes. Their application in high-speed pectic enzyme fractionation. Process Biochem. 2004; 39: 1017-1024.
36. García MC, Marina ML, Torre M. Perfusion chromatography: an emergent technique for the analysis of food proteins. J Chromatogr A. 2000; 880: 169-187.
37. Du KF, Yang D, Sun Y. Fabrication of high-permeability and high-capacity monolith for protein chromatography. J Chromatogr A. 2007; 1163: 212-218.
38. de Andrade Lima LRP. Liquid axial dispersion and holdup in column leaching. Mineral Eng. 2006; 19: 37-47.