Agrobacterium-mediated genetic transformation of economically important oilseed rape cultivars

Plant Cell, Tissue and Organ Culture

Volumn 107, Issue 2, 2011, Pages 317-323

  Boszoradova, Eva ^a   Libantova, Jana ^a   Matusikova, Ildiko ^a   Poloniova, Zuzana ^a   Jopcik, Martin ^a   Berenyi, Maria ^b   Moravcikova, Jana ^a  

^a INSTITUTE OF CHEMISTRY   (Slovakia)

^b AIT AUSTRIAN INSTITUTE OF TECHNOLOGY   (Austria)

Author keywords

glucuronidase; Agrobacterium tumefaciens; Brassica napus; Cotyledonary petioles; Hypocotyls

Indexed keywords

AGROBACTERIUM TUMEFACIENS; BRASSICA NAPUS; COTYLEDONARY PETIOLES; GLUCURONIDASE; HYPOCOTYLS;

CULTIVATION; GENES; GENETIC ENGINEERING; NUCLEIC ACIDS; OILSEEDS;

PLANTS (BOTANY);

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; BRASSICA NAPUS; HEROS; RHIZOBIUM;

EID: 80053921988     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-011-9982-y     Document Type: Article

References (52)

1. Abdollahi MR, Corral-Martinez P, Mousavi A, Salmanian AH, Moieni A, Segui-Simarro JM (2009) An efficient method for transformation of pre-androgenic, isolated Brassica napus microspores involving microprojectile bombardment and Agrobacterium-mediated transformation. Acta Physiol Plant 31: 1313-1317.
2. Abdollahi MR, Moieni A, Mousavi A, Salmanian AH (2011) High frequency production of rapeseed transgenic plants via combination of microprojectile bombardment and secondary embryogenesis of microspore-derived embryos. Mol Biol Rep 38: 711-719.
3. Bhowmik P, Dirpaul J, Polowick P, Ferrie AMR (2011) A high throughput Brassica napus microspore culture system: influence of percoll gradient separation and bud selection on embryogenesis. Plant Cell Tiss Org. doi: 10. 1007/s11240-009-9610-2: 1-4.
4. Boulter ME, Croy E, Simpson P, Shields R, Croy RRD, Shirsat AH (1990) Transformation of Brassica napus L (oilseed rape) using Agrobacterium tumefaciens and Agrobacterium rhizogenes: a comparison. Plant Sci 70: 91-99.
5. Cardoza V, Stewart CN (2003) Increased Agrobacterium-mediated transformation and rooting efficiencies in canola (Brassica napus L.) from hypocotyl segment explants. Plant Cell Rep 21: 599-604.
6. Charest PJ, Holbrook LA, Gabard J, Iyer VN, Miki BL (1988) Agrobacterium-mediated transformation of thin cell layer explants from Brassica napus L. Theor Appl Gen 75: 438-445.
7. Chen JL, Beversdorf WD (1994) A combined use of microprojectile bombardment and DNA imbibition enhances transformation frequency of canola (Brassica napus L.). Theor Appl Gen 88: 187-192.
8. Chen Z, Greenblatt IM, Dellaporta SL (1992) Molecular analysis of Ac transposition and DNA replication. Genetics 130: 665-676.
9. Colby SM, Juncosa AM, Meredith CP (1991) Cellular differences in Agrobacterium susceptibility and regenerative capacity restrict the development of transgenic grapevines. J Am Soc Hortic Sci 116: 356-361.
10. Damgaard O, Jensen LH, Rasmussen OS (1997) Agrobacterium tumefaciens-mediated transformation of Brassica napus winter cultivars. Transgenic Res 6: 279-288.
11. De Block M, Debrouwer D (1991) 2 T-DNA's co-transformed into Brassica napus by a double Agrobacterium tumefaciens infection are mainly integrated at the same locus. Theor Appl Gen 82: 257-263.
12. De Block M, Debrouwer D, Tenning P (1989) Transformation of Brassica napus and Brassica oleracea using Agrobacteriumtumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol 91: 694-701.
13. Ferrie AMR, Möllers C (2011) Haploids and doubled haploids in Brassica spp. For genetic and genomic research. Plant Cell Tiss Org 104: 375-386.
14. Fry J, Barnason A, Horsch RB (1987) Transformation of Brassica napus with Agrobacterium tumefaciens based vectors. Plant Cell Rep 6: 321-325.
15. Ghnaya AB, Charles G, Branchard M (2008) Rapid shoot regeneration from thin cell layer explants excised from petioles and hypocotyls in four cultivars Brassica napus L. Plant Cell Tiss Org 92: 25-30.
16. Guerche P, Charbonnier M, Jouanin L, Tourneur C, Paszkowski J, Pelletier G (1987) Direct gene transfer by electroporation in Brassica napus. Plant Sci 52: 111-116.
17. Hong HP, Gerster JL, Datla RSS, Albani D, Scoles G, Keller W, Robert LS (1997) The promoter of a Brassica napus polygalacturonase gene directs pollen expression of beta-glucuronidase in transgenic Brassica plants. Plant Cell Rep 16: 373-378.
18. Xooc-encoding hrf2 gene. Transgenic Res 19: 841-847.
19. Hüsken A, Baumert A, Milkowski C, Becker HC, Strack D, Mollers C (2005a) Resveratrol glucoside (piceid) synthesis in seeds of transgenic oilseed rape (Brassica napus L.). Theor Appl Gen 111: 1553-1562.
20. Hüsken A, Baumert A, Strack D, Becker HC, Möllers C, Milkowski C (2005b) Reduction of sinapate ester content in transgenic oilseed rape (Brassica napus) by dsRNAi-based suppression of BnSGT1 gene expression. Mol Breeding 16: 127-138.
21. Jefferson RA, Kavanagh TA, Bevan MW (1987) Gus fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901-3907.
22. Kamal GB, Illich KG, Asadollah A (2007) Effects of genotype explant type and nutrient medium components on canola (Brassica napus L.) shoot in vitro organogenesis. Afr J Biotechnol 6: 861-867.
23. Khan MR, Rashid H, Ansar M, Chaudry Z (2003) High frequency shoot regeneration and Agrobacterium-mediated DNA transfer in Canola (Brassica napus). Plant Cell Tiss Org 75: 223-231.
24. Khan I, Ali W, Takar ZA Frooqi A, Sikandar WA (2010) Increased regeneration efficiency of Brassica napus L. cultivars Star, Westar and Cyclone from hypocotyle and cotyledonary explants. Available from Nature Precedings http://hdl. handle. net/10101/npre. 2010. 4781. 1.
25. Kong FM, Li J, Tan XL, Zhang LL, Zhang ZY, Qi CK, Ma XK (2009) A new time-saving transformation system for Brassica napus. Afr J Biotechnol 8: 2497-2502.
26. Kopertekh L, Broer I, Schiemann J (2009) Developmentally regulated site-specific marker gene excision in transgenic B napus plants. Plant Cell Rep 28: 1075-1083.
27. Li X, Ahlman A, Yan X, Lindgren H, Zhu LH (2010) Genetic transformation of the oilseed crop Crambe abyssinica. Plant Cell Tiss Org 100: 149-156.
28. Liu H, Guo X, Naeem MS, Liu D, Xu L, Zhang W, Tang G, Zhou W (2010) Transgenic Brassica napus L. lines carrying a two gene construct demonstrate enhanced resistance against Plutella xylostella and Sclerotinia sclerotiorum. Plant Cell Tiss Org. doi 10. 1007/s11240-010-9902-6.
29. Miki B, Huang B, Bird S, Kemble R, Simmonds D, Keller W (1989) A procedure for the microinjection of plant cells and protoplasts. Methods Cell Sci 12: 139-144.
30. Moloney MM, Walker JM, Sharma KK (1989) High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep 8: 238-242.
31. Murashige I, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol 15: 473-497.
32. Nath UK, Wilmer JA, Wallington EJ, Becker HC, Möllers C (2009) Increasing erucic acid content through combination of endogenous low polyunsaturated fatty acids alleles with Ld-LPAAT + Bn-fae1 transgenes in rapeseed (Brassica napus L.). Theor Appl Gen 118: 765-773.
33. Ovesná J, Ptáček L, Opatrný Z (1993) Factors influencing the regeneration capacity of oilseed rape and cauliflower in transformation experiments. Biol Plant 35: 107-112.
34. Pandian A, Hurlstone C, Liu Q, Singh S, Salisbury P, Green A (2006) Agrobacterium- mediated transformation protocol to overcome necrosis in elite Australian Brassica juncea lines. Plant Mol Biol Rep 24: 103a-103i.
35. Pechan PM (1989) Successful cocultivation of Brassica napus microspores and proembryos with Agrobacterium. Plant Cell Rep 8: 387-390.
36. Peng Q, Hu Y, Wei R, Zhang Y, Guan CY, Ruan Y, Liu CL (2010) Simultaneous silencing of FAD2 and FAE1 genes affects both oleic acid and erucic acid contents in Brassica napus seeds. Plant Cell Rep 29: 317-325.
37. Pua EC, Mehrapalta A, Nagy F, Chua NH (1987) Transgenic plants of Brassica napus L. Bio-Technol 5: 815-817.
38. Radke SE, Andrews BM, Moloney MM, Crouch ML, Kridl JC, Knauf VC (1988) Transformation of Brassica napus L. using Agrobacterium tumefaciens: developmentally regulated expression of a reintroduced napin gene. Theor Appl Gen 75: 685-694.
39. Reiss E, Schubert J, Scholze P, Kramer R, Sonntag K (2009) The barley thaumatin-like protein HV-TLP8 enhances resistance of oilseed rape plants to Plasmodiophora brassicae. Plant Breed 128: 210-212.
40. Salaj T, Moravcikova J, Vookova B, Salaj J (2009) Agrobacterium-mediated transformation of embryogenic tissues of hybrid firs (Abiesspp.) and regeneration of transgenic emblings. Biotechnol Lett 31: 647-652.
41. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Press, Cold Spring Harbor, New York. ISBN 0-87969-309-6.
42. Schröder M, Dixelius C, Rahlen L, Glimelius K (1994) Transformation of Brassica napus by using the aadA gene as selectable marker and inheritance studies of the marker genes. Physiol Plantarum 92: 37-46.
43. Shi Y, Xu G, Warrington TB, Murdoch GK, Kazala EC, Snyder CL, Weselake RJ (2008) Microspore-derived cell suspension cultures of oilseed rape as a system for studying gene expression. Plant Cell Tiss Org 92: 131-139.
44. Spangenberg G, Neuhaus G, Schweiger HG (1986) Expression of foreign genes in a higher plant cell after electrofusion-mediated cell reconstruction of a microinjected karyoplast and a cytoplast. Eur J Cell Biol 42: 236-238.
45. Tang GX, Zhou WJ, Li HZ, Mao BZ, He ZH, Yoneyama K (2003) Medium, explant and genotype factors influencing shoot regeneration in oilseed Brassica spp. J Agron Crop Sci 189: 351-358.
46. Wallbraun M, Sonntag K, Eisenhauer C, Krzcal G, Wang YP (2009) Phosphomannose-isomerase (pmi) gene as a selectable marker for Agrobacterium-mediated transformation of rapeseed. Plant Cell Tiss Org 99: 345-351.
47. Wang J, Chen Z, Du J, Sun Y, Liang Y (2005) Novel insect resistance in Brassica napus developed by transformation of chitinase and scorpion toxin genes. Plant Cell Rep 24: 549-555.
48. Wang M, Liu M, Li D, Wu J, Li X, Yang Y (2010) Overexpression of FAD2 promotes seed germination and hypocotyl elongation in Brassica napus. Plant Cell Tiss Org 102: 205-211.
49. Weselake RJ, Shah S, Tang MG, Quant PA, Snyder CL, Furukawa-Stoffer TL, Zhu WM, Taylor DC, Zou JT, Kumar A, Hall L, Laroche A, Rakow G, Raney P, Moloney MM, Harwood JL (2008) Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content. J Expl Bot 59: 3543-3549.
50. Zhang HX, Hodson JN, Williams JP, Blumwald E (2001) Engineering salt-tolerant Brassica plants: Characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation. Proc Natl Acad Sci USA 98: 12832-12836.
51. Zhang Y, Singh MB, Swoboda I, Bhalla PL (2005) Agrobacterium-mediated transformation and generation of male sterile lines of australian canola. Aust J Agr Res 56: 353-361.
52. Zhang Y, Xu J, Han L, Wei W, Guan Z, Cong L, Chai T (2006) Efficient shoot regeneration and Agrobacterium-mediated transformation of Brassica juncea. Plant Mol Biol Rep 24: 255a-255i.