Changing the direction and orientation of electric field during electric pulses application improves plasmid gene transfer in vitro

Journal of Visualized Experiments

Volumn , Issue 55, 2011, Pages

  Pavlin, Mojca ^a   Haberl, Saša ^a   Reberšek, Matej ^a   Miklavčič, Damijan ^a   Kandušer, Maša ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

Changing the orientation of electric field; Gene; Gene electrotransfer; Gfp; Issue 55; Medicine; Plasmid; Transfection

Indexed keywords

EID: 80053915557     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3309     Document Type: Article

References (14)

1. Rebersek, M., et al. Electroporator with automatic change of electric field direction improves gene electrotransfer in-vitro. Biomed Eng Online. 6, 25 (2007).
2. Trontelj, K., Rebersek, M., & Miklavcic, D. Tip electrode chamber for small volume electroporation, electrofusion, and gene transfection. 18, PCT/SI2007/000036 (2006).
3. Neumann, E., Schaefer-Ridder, M., Wang, Y., & Hofschneider, P. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1, 841 (1982).
4. Daud, A., et al. Phase I trial of interleukin-12 plasmid electroporation in patients with metastatic melanoma. J Clin Oncol. 26, 5896-5903 (2008).
5. Pavlin, M. & Miklavcic, D. Effective conductivity of a suspension of permeabilized cells: a theoretical analysis. Biophys J. 85, 719-729 (2003).
6. Xie, T., Sun, L., Zhao, H., Fuchs, J., & Tsong, T. Study of mechanisms of electric field-induced DNA transfection. IV. Effects of DNA topology on cell uptake and transfection efficiency. Biophys J. 63, 1026-1031 (1992).
7. Rols, M., Delteil, C., Serin, G. & Teissie, J. Temperature effects on electrotransfection of mammalian cells. Nucleic Acids Res. 22, 540 (1994).
8. Golzio, M., Teissie, J. & Rols, M. Cell synchronization effect on mammalian cell permeabilization and gene delivery by electric field. Biochim Biophys Acta. 1563, 23-28 (2002).
9. Haberl, S., Miklavcic, D. & Pavlin, M. Effect of Mg ions on efficiency of gene electrotransfer and on cell electropermeabilization. Bioelectrochemistry. 79, 265-271 (2010).
10. Rols, M. & Teissie, J. Electropermeabilization of mammalian cells to macromolecules: control by pulse duration. Biophys J. 75, 1415-1423 (1998).
11. Kanduser, M., Miklavcic, D. & Pavlin, M. Mechanisms involved in gene electrotransfer using high-and low-voltage pulses-An in vitro study. Bioelectrochemistry. 74, 265-271 (2009).
12. Pavlin, M., Flisar, K. & Kanduser, M. The role of electrophoresis in gene electrotransfer. J Membr Biol. 236, 75-79 (2010).
13. Sersa, G., Cemazar, M., Semrov, D. & Miklavcic, D. Changing electrode orientation improves the efficacy of electrochemotherapy of solid tumors in mice. Bioelectrochem Bioenerg. 39, 61-66 (1996).
14. Faurie, C. et al. Electro-mediated gene transfer and expression are controlled by the life-time of DNA/membrane complex formation. J Gene Med. 12, 117-125 (2010).