Basic techniques in human mesenchymal stem cell cultures: Differentiation into osteogenic and adipogenic lineages, genetic perturbations, and phenotypic analyses

Current Protocols in Stem Cell Biology

Volumn , Issue SUPPL. 17, 2011, Pages

  Bruedigam, Claudia ^a   van Driel, Marjolein ^a   Koedam, Marijke ^a   van de Peppel, Jeroen ^a   van der Eerden, Bram C J ^a   Eijken, Marco ^a   van Leeuwen, Johannes P T M ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

Adipocyte; Alkaline phosphatase; Gene expression analysis; Human mesenchymal stem cell; In vitro differentiation; Lipid vesicle; Mineralization; Osteoblast

Indexed keywords

ALKALINE PHOSPHATASE; CELL DNA; DEXAMETHASONE; FAT DROPLET; GLYCEROL 2 PHOSPHATE; INDOMETACIN; ISOBUTYLMETHYLXANTHINE; RNA; BIOLOGICAL MARKER; DNA; LIPID;

ADIPOCYTE; ADIPOGENESIS; ARTICLE; BIOMINERALIZATION; BONE DEVELOPMENT; CELL DIFFERENTIATION; CENTRIFUGATION; CULTURE TECHNIQUE; ELECTROPORATION; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC TRANSFECTION; HISTOPATHOLOGY; HUMAN; HUMAN CELL; IN VITRO STUDY; MESENCHYMAL STEM CELL; OSTEOBLAST; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; BONE MINERALIZATION; CELL CULTURE; CELL LINEAGE; CHEMISTRY; CYTOLOGY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; METHODOLOGY;

ADIPOGENESIS; ALKALINE PHOSPHATASE; BIOLOGICAL MARKERS; CALCIFICATION, PHYSIOLOGIC; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINEAGE; CELLS, CULTURED; DNA; ELECTROPORATION; GENE EXPRESSION REGULATION; HUMANS; LIPIDS; MESENCHYMAL STEM CELLS; OSTEOGENESIS; PHENOTYPE; TRANSFECTION;

EID: 80053897882     PISSN: 19417322     EISSN: 19388969     Source Type: Journal    
DOI: 10.1002/9780470151808.sc01h03s17     Document Type: Article

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