Quantitative real-time polymerase chain reaction improves conventional microbiological diagnosis in an outbreak of brucellosis due to ingestion of unpasteurized goat cheese

Diagnostic Microbiology and Infectious Disease

Volumn 71, Issue 3, 2011, Pages 294-296

  Colmenero, Juan D ^a   Clavijo, Encarnación ^b   Morata, Pilar ^c   Bravo, María J ^a,d   Queipo Ortuño, María I ^a,d  

^a HOSPITAL REGIONAL UNIVERSITARIO DE MÁLAGA   (Spain)

^b HOSPITAL UNIVERSITARIO VIRGEN DE LA VICTORIA   (Spain)

^c UNIVERSITY OF MÁLAGA   (Spain)

^d Hospital University Carlos Haya de Málaga   (Spain)

Author keywords

Brucellosis; Diagnosis; Epidemiology; Outbreak; Real time PCR

Indexed keywords

ROSE BENGAL;

ADOLESCENT; ADULT; AGGLUTINATION TEST; ARTHRALGIA; ARTICLE; BLOOD CULTURE; BRUCELLA MELITENSIS; BRUCELLOSIS; CHEESE; CHILD; CHILL; CLINICAL ARTICLE; DIAGNOSTIC VALUE; FEVER; FOOD INTAKE; HUMAN; MALAISE; MALE; MICROBIOLOGICAL EXAMINATION; PHYSICAL EXAMINATION; PRESCHOOL CHILD; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCHOOL CHILD; SWEATING;

ADOLESCENT; ADULT; ANIMALS; BRUCELLA MELITENSIS; BRUCELLOSIS; CHEESE; CHILD; DISEASE OUTBREAKS; EATING; FAMILY HEALTH; FEMALE; GENES, BACTERIAL; GOATS; HUMANS; MALE; MIDDLE AGED; REAL-TIME POLYMERASE CHAIN REACTION; YOUNG ADULT;

EID: 80053892657     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2011.06.016     Document Type: Article

References (13)

1. Alton G.G., Jones L.M., Pietz D.E. Laboratory techniques in brucellosis 1975, World Health Organization, Geneva, Switzerland. 2nd ed.
2. Corbel M.J. Brucellosis an overview. Emerg. Infect. Dis. 1997, 3:213-221.
3. Navarro E., Segura J.C., Castaño M.J., Solera J. Use of real-time quantitative polymerase chain reaction to monitor the evolution of Brucella melitensis DNA load during therapy and post-therapy follow-up in patients with brucellosis. Clin. Infect. Dis. 2006, 42:1266-1273.
4. Orduña A., Almaraz A., Prado A., Gutierrez M.P., Garcia-Pascual A., Dueñas A., Cuervo M., Abad R., Hernández B., Lorenzo B., Bratos M.A., Torres A.R. Evaluation of an immunocapture-agglutination test (BrucellaCapt) for the serodiagnosis of human brucellosis. J. Clin. Microbiol. 2000, 38:4000-4005.
5. Pappas G., Akritidis N., Bosilkovski M., Tsianos E. Brucellosis. N. Engl. J. Med. 2005, 352:2325-2336.
6. Queipo-Ortuño M.I., Colmenero J.D., Bravo M.J., García-Ordoñez M.A., Morata P. Usefulness of a quantitative real-time PCR assay using serum samples to discriminate between inactive, serologically positive and active human brucellosis. Clin. Microbiol. Infect. 2008, 14:1128-1134.
7. Ramos J.M., Bernal E., Esguevillas T., Lopez-Garcia P., Gaztambide M.S., Gutierrez F. Non-imported brucellosis outbreak from unpasteurized raw milk in Moroccan immigrants in Spain. Epidemiol. Infect. 2008, 136:1552-1555.
8. Staszkiewicz J., Lewis C.M., Colville J., Zervos M., Band J. Outbreak of Brucella melitensis among microbiology laboratory workers in a community hospital. J. Clin. Microbiol. 1991, 29:287-290.
9. Trout D., Gomez T.M., Bernard B.P., Mueller C.A., Smith C.G., Hunter L., Kiefer M. Outbreak of brucellosis at a United States pork packing plant. J. Occup. Environ. Med. 1995, 37:697-703.
10. Wallach J.C., Samartino L.E., Efron A., Baldi P.C. Urban outbreak of a Brucella melitensis infection in an Argentine family: clinical and diagnostic aspects. FEMS Immunol. Med. Microbiol. 1994, 8:49-56.
11. Wallach J.C., Miguel S.E., Baldi P.C., Guarnera E., Goldbaum F.A., Fossati C.A. Human infection by Brucella melitensis: an outbreak attributed to contact with infected goats. FEMS Immunol. Med. Microbiol. 1997, 19:315-321.
12. Yagupsky P., Baron E.J. Laboratory exposures to brucellae and implications for bioterrorism. Emerg. Infect. Dis. 2005, 11:1180-1185.
13. Young E.J. An overview of human brucellosis. Clin. Infect. Dis. 1995, 21:283-289.