Culture of corneal limbal epithelial stem cells: Experience from benchtop to bedside in a tertiary care hospital in India

Cornea

Volumn 30, Issue 11, 2011, Pages 1223-1232

  Sharma, Shweta ^a   Tandon, Radhika ^a   Mohanty, Sujata ^b   Sharma, Namrata ^a   Vanathi, M ^a   Sen, Seema ^a   Kashyap, Seema ^a   Singh, Neeta ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

^b ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

cornea; limbal epithelial stem cells; transplantation

Indexed keywords

BREAST CANCER RESISTANCE PROTEIN; CONNEXIN 43; CYTOKERATIN 12; CYTOKERATIN 19; PROTEIN P63; TRANSCRIPTION FACTOR PAX6;

ADOLESCENT; ADULT; AMNION; ARTICLE; CADAVER DONOR; CELL GROWTH; CHEMICAL INJURY; CHILD; CORNEA CELL; CORNEA DISEASE; CORNEA LIMBUS; CULTURE MEDIUM; DISEASE SEVERITY; EX VIVO STUDY; EYE BURN; FEMALE; FOLLOW UP; HISTOPATHOLOGY; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOGENICITY; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; INDIA; LIMBAL EPITHELIAL STEM CELL; LIMBAL EPITHELIAL STEM CELL DEFICIENCY; LIMBAL STEM CELL TRANSPLANTATION; LIVING DONOR; MAJOR CLINICAL STUDY; MALE; OUTCOME ASSESSMENT; PRESCHOOL CHILD; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCHOOL CHILD; STEM CELL; STEVENS JOHNSON SYNDROME; TERTIARY HEALTH CARE; VISUAL ACUITY;

ADOLESCENT; ADULT; AMNION; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; CHILD; CHILD, PRESCHOOL; CORNEAL DISEASES; EPITHELIAL CELLS; EPITHELIUM, CORNEAL; FEMALE; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; HOSPITALS; HUMANS; IMMUNOENZYME TECHNIQUES; INDIA; LIMBUS CORNEAE; MALE; MIDDLE AGED; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STEM CELL TRANSPLANTATION; STEM CELLS; TISSUE DONORS; VISUAL ACUITY; YOUNG ADULT;

EID: 80053622692     PISSN: 02773740     EISSN: 15364798     Source Type: Journal    
DOI: 10.1097/ICO.0b013e3181dc81f1     Document Type: Article

References (26)

1. Dua HS, Azuara-Blanco A. Limbal stem cells of the corneal epithelium. Surv Ophthalmol. 2000;44:415-425. (Pubitemid 30151147)
2. Pellegrini G, Traverso CE, Franzi AT, et al. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet. 1997;349:990-993. (Pubitemid 27149803)
3. Schwab IR. Cultured corneal epithelia for ocular surface disease. Trans Am Ophthalmol Soc. 1999;97:891-986. (Pubitemid 30106658)
4. Tsai RJ, Li LM, Chen JK. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N Engl J Med. 2000; 343:86-93. (Pubitemid 30451851)
5. Koizumi N, Inatomi T, Suzuki T, et al. Cultivated corneal epithelial stem cell transplantation in ocular surface disorders. Ophthalmology. 2001;108: 1569-1574. (Pubitemid 32808330)
6. Rama P, Bonini S, Lambiase A, et al. Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency. Transplantation. 2001;72:1478-1485. (Pubitemid 33095504)
7. Meller D, Pires RT, Tseng SC. Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. Br J Ophthalmol. 2002;86:463-471. (Pubitemid 34293299)
8. Shimazaki J, Aiba M, Goto E, et al. Transplantation of human limbal epithelium cultivated on amniotic membrane for the treatment of severe ocular surface disorders. Ophthalmology. 2002;109:1285-1290. (Pubitemid 34700919)
9. Sangwan VS, Vemuganti GK, Iftekhar G, et al. Use of autologous cultured limbal and conjunctival epithelium in a patient with severe bilateral ocular surface disease induced by acid injury: A case report of unique application. Cornea. 2003;22:478-481. (Pubitemid 36759044)
10. Grueterich M, Espana EM, Touhami A, et al. Phenotypic study of a case with successful transplantation of ex vivo expanded human limbal epithelium for unilateral total limbal stem cell deficiency. Ophthalmology. 2002;109:1547-1552.
11. Sangwan VS, Vemuganti GK, Singh S, et al. Successful reconstruction of damaged ocular outer surface in humans using limbal and conjuctival stem cell culture methods. Biosci Rep. 2003;23:169-174. (Pubitemid 38102029)
12. Daya SM, Watson A, Sharpe JR, et al. Outcomes and DNA analysis of ex vivo expanded stem cell allograft for ocular surface reconstruction. Ophthalmology. 2005;112:470-477. (Pubitemid 40312503)
13. Sangwan VS, Matalia HP, Vemuganti GK, et al. Clinical outcome of autologous cultivated limbal epithelium transplantation. Indian J Ophthalmol. 2006;54:29-34. (Pubitemid 43363573)
14. Shortt AJ, Secker GA, Rajan MS, et al. Ex vivo expansion and transplantation of limbal epithelial stem cells. Ophthalmology. 2008;115:1989-1997.
15. Nakamura T, Inatomi T, Sotozono C, et al. Transplantation of autologous serum-derived cultivated corneal epithelial equivalents for the treatment of severe ocular surface disease. Ophthalmology. 2006;113:1765-1772. (Pubitemid 44442442)
16. Nakamura T, Inatomi T, Sotozono C, et al. Successful primary culture and autologous transplantation of corneal limbal epithelial cells from minimal biopsy for unilateral severe ocular surface disease. Acta Ophthalmol Scand. 2004;82:468-471. (Pubitemid 39199451)
17. de Paiva CS, Chen Z, Corrales RM, et al. ABCG2 transporter identifies a population of clonogenic human limbal epithelial cells. Stem Cells. 2005;23:63-73. (Pubitemid 40105292)
18. Wolosin JM. Cell markers and side population phenotype in ocular surface epithelial stem cells characterization and isolation. Ocul Surf. 2006;4:10-23. (Pubitemid 43285940)
19. Budak MT, Alpdogan OS, Zhou M, et al. Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells. J Cell Sci. 2005;118:1715-1724. (Pubitemid 40691899)
20. Tseng SC. Concept and application of limbal stem cells. Eye. 1989;3: 141-157. (Pubitemid 20018556)
21. Daniels JT, Harris AR, Mason C. Corneal epithelial stem cells in health and disease. Stem Cell Rev. 2006;2:247-254. (Pubitemid 47109403)
22. Shortt AJ, Secker GA, Notara MD, et al. Transplantation of ex vivo cultured limbal epithelial stem cells: A review of techniques and clinical results. Surv Ophthalmol. 2007;52:483-502. (Pubitemid 47268554)
23. Vemuganti GK, Kashyap S, Sangwan VS, et al. Ex-vivo potential of cadaveric and fresh limbal tissues to regenerate cultured epithelium. Indian J Ophthalmol. 2004;52:113-120. (Pubitemid 38915498)
24. Schermer A, Galvin S, Sun TT. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J Cell Biol. 1986;103:49-62. (Pubitemid 16066991)
25. Arpitha P, Prajna NV, Srinivasan M, et al. High expression of p63 combined with a large N/C ratio defines a subset of human limbal epithelial cells: Implications on epithelial stem cells. Invest Ophthalmol Vis Sci. 2005;46:3631-3636. (Pubitemid 44264666)
26. Kivelä T, Uusitalo M. Structure, development and function of cytoskeletal elements in non-neuronal cells of the human eye. Prog Retin Eye Res. 1998;17:385-428. (Pubitemid 28342388)