Frequent mutations within the genomic magnetosome island of magnetospirillum gryphiswaldense are mediated by RecA

Journal of Bacteriology

Volumn 193, Issue 19, 2011, Pages 5328-5334

  Kolinko, Isabel ^a   Jogler, Christian ^a,b   Katzmann, Emanuel ^a   Schüler, Dirk ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OXYGEN; RECA PROTEIN;

ALLELE; ARTICLE; BACTERIAL GENOME; BACTERIAL STRAIN; BIOTECHNOLOGY; GENE DELETION; GENE EXPRESSION; GENE MUTATION; GENE REARRANGEMENT; GENETIC ENGINEERING; GENETIC STABILITY; HOMOLOGOUS RECOMBINATION; MAGNETOSOME; MAGNETOSPIRILLUM GRYPHISWALDENSE; METABOLIC STRESS; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; ULTRAVIOLET IRRADIATION; WILD TYPE;

BACTERIAL PROTEINS; GENOMIC ISLANDS; MAGNETOSOMES; MAGNETOSPIRILLUM; MICROSCOPY, ELECTRON, TRANSMISSION; MUTATION; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS); MAGNETOSPIRILLUM GRYPHISWALDENSE;

EID: 80053584669     PISSN: 00219193     EISSN: 10985530     Source Type: Journal    
DOI: 10.1128/JB.05491-11     Document Type: Article

References (47)

1. Bender, R. A. 1984. Ultraviolet mutagenesis and inducible DNA repair in Caulobacter crescentus. Mol. Gen. Genet. 197:399-402.
2. Berson, A. E., D. V. Hudson, and N. S. Waleh. 1989. Cloning and characterization of the recA gene of Aquaspirillum magnetotacticum. Arch. Microbiol. 152:567-571.
3. Berson, A. E., M. R. Peters, and N. S. Waleh. 1990. Nucleotide sequence of recA gene of Aquaspirillum magnetotacticum. Nucleic Acids Res. 18:675.
4. Bertani, G. 2004. Lysogeny at mid-twentieth century: P1, P2, and other experimental systems. J. Bacteriol. 186:595-600.
5. Bull, C. T., et al. 2001. Characterization of spontaneous gacS and gacA regulatory mutants of Pseudomonas fluorescens biocontrol strain CHAO. Antonie Van Leeuwenhoek 79:327-336.
6. Capaldo, F. N., G. Ramsey, and S. D. Barbour. 1974. Analysis of the growth of recombination-deficient strains of Escherichia coli K-12. J. Bacteriol. 118: 242-249.
7. Cheng, P., and H. Michel. 1998. Cloning, sequencing, and characterization of the recA gene from Rhodopseudomonas viridis and construction of a recA strain. J. Bacteriol. 180:3227-3232.
8. Clark, A. J., and A. D. Margulies. 1965. Isolation and characterization of recombination-deficient mutants of Escherichia coli K12. Proc. Natl. Acad. Sci. U. S. A. 53:451-459.
9. Dutra, B. E., V. A. Sutera, Jr., and S. T. Lovett. 2007. RecA-independent recombination is efficient but limited by exonucleases. Proc. Natl. Acad. Sci. U. S. A. 104:216-221.
10. Duwat, P., S. D. Ehrlich, and A. Gruss. 1995. The recA gene of Lactococcus lactis: characterization and involvement in oxidative and thermal stress. Mol. Microbiol. 17:1121-1131.
11. Farrand, S. K., S. P. O'Morchoe, and J. McCutchan. 1989. Construction of an Agrobacterium tumefaciens C58 recA mutant. J. Bacteriol. 171:5314-5321.
12. Fox, A., et al. 2008. Emergence of secretion-defective sublines of Pseudomonas aeruginosa PAO1 resulting from spontaneous mutations in the vfr global regulatory gene. Appl. Environ. Microbiol. 74:1902-1908.
13. Heyen, U., and D. Schüler. 2003. Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor. Appl. Microbiol. Biotechnol. 61:536-544.
14. Horii, T., T. Ogawa, and H. Ogawa. 1980. Organization of the recA gene of Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 77:313-317.
15. Jogler, C., et al. 2009. Comparative analysis of magnetosome gene clusters in magnetotactic bacteria provides further evidence for horizontal gene transfer. Environ. Microbiol. 11:1267-1277.
16. Jogler, C., et al. 2009. Toward cloning of the magnetotactic metagenome: identification of magnetosome island gene clusters in uncultivated magnetotactic bacteria from different aquatic sediments. Appl. Environ. Microbiol. 75:3972-3979.
17. Jogler, C., et al. 2010. Cultivation-independent characterization of 'Candidatus Magnetobacterium bavaricum' via ultrastructural, geochemical, ecological and metagenomic methods. Environ. Microbiol. 12:2466-2478.
18. Jogler, C., et al. 2011. Conservation of proteobacterial magnetosome genes and structures in an uncultivated member of the deep-branching Nitrospira phylum. Proc. Natl. Acad. Sci. U. S. A. 108:1134-1139.
19. Kovach, M. E., et al. 1995. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175-176.
20. Marx, C. J., and M. E. Lidstrom. 2002. Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria. Biotechniques 33: 1062-1067.
21. Michel, B. 2005. After 30 years of study, the bacterial SOS response still surprises us. PLoS Biol. 3:e255.
22. Murat, D., A. Quinlan, H. Vali, and A. Komeili. 2010. Comprehensive genetic dissection of the magnetosome gene island reveals the step-wise assembly of a prokaryotic organelle. Proc. Natl. Acad. Sci. U. S. A. 107:5593- 5598.
23. Muth, G., D. Frese, A. Kleber, and W. Wohlleben. 1997. Mutational analysis of the Streptomyces lividans recA gene suggests that only mutants with residual activity remain viable. Mol. Gen. Genet. 255:420-428.
24. Nakazawa, H., et al. 2009. Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria. Genome Res. 19:1801-1808.
25. Neidhardt, F. C., et al. (ed.). 1996. Escherichia coli and Salmonella: cellular and molecular biology, 2nd ed., vol. 2. ASM Press, Washington, DC.
26. O'Neill, E. A., R. H. Hynes, and R. A. Bender. 1985. Recombination deficient mutant of Caulobacter crescentus. Mol. Gen. Genet. 198:275-278.
27. Richter, M., et al. 2007. Comparative genome analysis of four magnetotactic bacteria reveals a complex set of group-specific genes implicated in magnetosome biomineralization and function. J. Bacteriol. 189:4899-4910.
28. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. Sander, P., et al. 2003. A recA deletion mutant of Mycobacterium bovis BCG confers protection equivalent to that of wild-type BCG but shows increased genetic stability. Vaccine 21:4124-4127.
30. Scheffel, A., A. Gardes, K. Grünberg, G. Wanner, and D. Schüler. 2008. The major magnetosome proteins MamGFDC are not essential for magnetite biomineralization in Magnetospirillum gryphiswaldense but regulate the size of magnetosome crystals. J. Bacteriol. 190:377-386.
31. Scheffel, A., and D. Schüler. 2007. The acidic repetitive domain of the Magnetospirillum gryphiswaldense MamJ protein displays hypervariability but is not required for magnetosome chain assembly. J. Bacteriol. 189:6437- 6446.
32. Schübbe, S., et al. 2003. Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum gryphiswaldense reveals a large deletion comprising a putative magnetosome island. J. Bacteriol. 185:5779-5790.
33. Schübbe, S., et al. 2006. Transcriptional organization and regulation of magnetosome operons in Magnetospirillum gryphiswaldense. Appl. Environ. Microbiol. 72:5757-5765.
34. Schüler, D. 2008. Genetics and cell biology of magnetosome formation in magnetotactic bacteria. FEMS Microbiol. Rev. 32:654-672.
35. Schüler, D. 2004. Molecular analysis of a subcellular compartment: the magnetosome membrane in Magnetospirillum gryphiswaldense. Arch. Microbiol. 181:1-7.
36. Schüler, D., R. R. Uhl, and E. Bäuerlein. 1995. A simple light scattering method to assay magnetism in Magnetospirillum gryphiswaldense. FEMS Microbiol. Ecol. 132:139-145.
37. Schultheiss, D., M. Kube, and D. Schüler. 2004. Inactivation of the flagellin gene flaA in Magnetospirillum gryphiswaldense results in nonmagnetotactic mutants lacking flagellar filaments. Appl. Environ. Microbiol. 70:3624-3631.
38. Schultheiss, D., and D. Schüler. 2002. Development of a genetic system for Magnetospirillum gryphiswaldense. Arch. Microbiol. 179:89-94.
39. Tchamedeu Kameni, A. P., E. Couture-Tosi, I. Saint-Girons, and M. Picardeau. 2002. Inactivation of the spirochete recA gene results in a mutant with low viability and irregular nucleoid morphology. J. Bacteriol. 184:452-458.
40. Ullrich, S., M. Kube, S. Schübbe, R. Reinhardt, and D. Schüler. 2005. A hypervariable 130-kilobase genomic region of Magnetospirillum gryphiswaldense comprises a magnetosome island which undergoes frequent rearrangements during stationary growth. J. Bacteriol. 187:7176-7184.
41. Ullrich, S., and D. Schüler. 2010. Cre-lox-based method for generation of large deletions within the genomic magnetosome island of Magnetospirillum gryphiswaldense. Appl. Environ. Microbiol. 76:2439-2444.
42. Viollier, E., P. W. Inglett, K. Hunter, A. N. Roychoudhury, and P. Van Cappellen. 2000. The ferrozine method revisited: Fe(II)/Fe(III) determination in natural waters. Appl. Geochem. 15:785-790.
43. Walker, G. C. 1984. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiol. Rev. 48:60-93.
44. Witkin, E. M. 1976. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol. Rev. 40:869-907.
45. Wollman, E. L., F. Jacob, and W. Hayes. 1956. Conjugation and genetic recombination in Escherichia coli K-12. Cold Spring Harb. Symp. Quant. Biol. 21:141-162.
46. Yang, W., et al. 2010. mamO and mamE genes are essential for magnetosome crystal biomineralization in Magnetospirillum gryphiswaldense MSR-1. Res. Microbiol. 161:701-705.
47. Zhao, L., D. Wu, L. F. Wu, and T. Song. 2007. A simple and accurate method for quantification of magnetosomes in magnetotactic bacteria by common spectrophotometer. J. Biochem. Biophys. Methods 70:377-383.