In vitro generation of osteochondral differentiation of human marrow mesenchymal stem cells in novel collagen-hydroxyapatite layered scaffolds

Acta Biomaterialia

Volumn 7, Issue 11, 2011, Pages 3999-4006

  Zhou, Jiaan ^a   Xu, Caixia ^a   Wu, Gang ^a   Cao, Xiaodong ^a   Zhang, Liangming ^b   Zhai, Zhichen ^a   Zheng, Zhiwen ^a   Chen, Xiaofeng ^a   Wang, Yingjun ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b SUN YAT SEN UNIVERSITY   (China)

Author keywords

Cartilage tissue engineering; Collagen; Hydroxyapatite; Mesenchymal stem cell; Osteochondral differentiation

Indexed keywords

BIOMIMETICS; CARTILAGE; CELL ENGINEERING; COLLAGEN; COMPOSITE MATERIALS; GENE EXPRESSION; HYDROXYAPATITE; OSTEOBLASTS; PHOSPHATASES; POLYMERASE CHAIN REACTION; SCAFFOLDS (BIOLOGY); STEM CELLS;

CARTILAGE TISSUE ENGINEERING; CHONDROGENIC DIFFERENTIATION; COMPOSITES MATERIAL; HUMAN MESENCHYMAL STEM CELLS; IMMUNOCYTOCHEMISTRY; IN-VITRO; MESENCHYMAL STEM CELL; OSTEOCHONDRAL; OSTEOCHONDRAL DIFFERENTIATION; OSTEOGENIC;

CELL CULTURE;

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ALCIAN BLUE; ALKALINE PHOSPHATASE; BIOMIMETIC MATERIAL; COLLAGEN; COLLAGEN TYPE 1; COLLAGEN TYPE 2; GLYCOSAMINOGLYCAN; HYDROXYAPATITE; MESSENGER RNA; OSTEOGENIC PROTEIN 1; TISSUE SCAFFOLD;

ARTICLE; BONE TISSUE; CARTILAGE CELL; CELL ASSAY; CELL DIFFERENTIATION; CELL GROWTH; CELL ISOLATION; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; CULTURE MEDIUM; FREEZE DRYING; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; MESENCHYMAL STEM CELL; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; TISSUE ENGINEERING;

EID: 80053560659     PISSN: 17427061     EISSN: 18787568     Source Type: Journal    
DOI: 10.1016/j.actbio.2011.06.040     Document Type: Article

References (25)

1. W. Swieszkowski, B.H.S. Tuan, K.J. Kurzydlowski, and D.W. Hutmacher Repair and regeneration of osteochondral defects in the articular joints Biomol Eng 24 2007 489 495 (Pubitemid 350123179)
2. J.M. Oliveira, M.T. Rodrigues, S.S. Silva, P.B. Malafaya, M.E. Gomes, and C.A. Viegas Novel hydroxyapatite/chitosan bilayered scaffold for osteochondral tissue-engineering applications: scaffold design and its performance when seeded with goat bone marrow stromal cells Biomaterials 27 2006 6123 6137 (Pubitemid 44429972)
3. R.M. Schek, J.M. Taboas, S.J. Segvich, S.J. Hollister, and P.H. Krebsbach Engineered osteochondral grafts using biphasic composite solid free-form fabricated scaffolds Tissue Eng 10 2004 1376 1385 (Pubitemid 39557842)
4. G. Xuan, P. Hansoo, L. Guangpeng, L. Wei, C. Yilin, and Y. Tabata In vitro chondrogenic differentiation of human mesenchymal stem cells in collagen microspheres: influence of cell seeding density and collagen concentration Biomaterials 2009 2741 2752
5. J. Mano, and R. Reis Osteochondral defects: present situation and tissue engineering approaches J Tissue Eng Regen M 1 2007 261 273
6. X. Guo, H. Park, G. Liu, W. Liu, Y. Cao, and Y. Tabata In vitro generation of an osteochondral construct using injectable hydrogel composites encapsulating rabbit marrow mesenchymal stem cells Biomaterials 30 2009 2741 2752
7. G. Wu, Y. Wang, X. Chen, J. Ye, C. Ning, and N. Zhao Fabrication of layered scaffold from cartilage tissue engineering Chin J Biomed Eng 113 2007 612 617 (Pubitemid 47262482)
8. H. Lu, D. Cai, G. Wu, and C. Zeng Construction of tissue engineering cartilage with collagen/hydroxyapatite composite scaffolds loaded chondrocytes in vitro Zhongguo Linchuang Kangfu (Chin J Clin Rehabil) 10 2006 177 178
9. M.F. Pittenger, J.D. Mosca, and K.R. McIntosh Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma Lymphoid Organogenesis 251 2000 3 11
10. B. Johnstone, and J.U. Yoo Autologous mesenchymal progenitor cells in articular cartilage repair Clin Orthop Relat Res 1999 S156 S162
11. G.I. Im, D.Y. Kim, J.H. Shin, C.W. Hyun, and W.H. Cho Repair of cartilage defect in the rabbit with cultured mesenchymal stem cells from bone marrow J Bone Joint Surg Br 83B 2001 289 294
12. S. Kadiyala, R.G. Young, M.A. Thiede, and S.P. Bruder Culture expanded canine mesenchymal stem cells possess osteochondrogenic potential in vivo and in vitro Cell Transplant 6 1997 125 134 (Pubitemid 27188295)
13. D.P. Lennon, S.E. Haynesworth, D.M. Arm, M.A. Baber, and A.I. Caplan Dilution of human mesenchymal stem cells with dermal fibroblasts and the effects on in vitro and in vivo osteochondrogenesis Dev Dyn 219 2000 50 62 (Pubitemid 30667123)
14. M.F. Pittenger, A.M. Mackay, S.C. Beck, R.K. Jaiswal, R. Douglas, and J.D. Mosca Multilineage potential of adult human mesenchymal stem cells Science 284 1999 143 147 (Pubitemid 29282067)
15. M.A. Mello, and R.S. Tuan High density micromass cultures of embryonic limb bud mesenchymal cells: an in vitro model of endochondral skeletal development In Vitro Cell Dev Biol Anim 35 1999 262 269 (Pubitemid 29252105)
16. R. Farndale, C. Sayers, and A. Barrett A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures Connect Tissue Res 9 1982 247 248 (Pubitemid 12009669)
17. J.I. Dawson, D.A. Wahl, S.A. Lanham, J.M. Kanczler, J.T. Czernuszka, and O. Roc Development of specic collagen scaffolds to support the osteogenic and chondrogenic differentiation of human bone marrow stromal cells Biomaterials 29 2008 3105 3116
18. Y. Stark, K. Suck, C. Kasper, M. Wieland, M. van Griensven, and T. Scheper Application of collagen matrices for cartilage tissue engineering Exp Toxicol Pathol 57 2006 305 311 (Pubitemid 43234015)
19. A. Bernhardt, A. Lode, C. Mietrach, U. Hempel, T. Hanke, and M. Gelinsky In vitro osteogenic potential of human bone marrow stromal cells cultivated in porous scaffolds from mineralized collagen J Biomed Mater Res A 90 2009 852 862
20. L.B. Rocha, G. Goissis, and M.A. Rossi Biocompatibility of anionic collagen matrix as scaffold for bone healing Biomaterials 23 2002 449 456 (Pubitemid 33051033)
21. J. Xie, M.J. Baumann, and L.R. McCabe Osteoblasts respond to hydroxyapatite surfaces sizes of pores controlling phenotype expression in BMP-induced osteogenesis with immediate changes in gene expression J Biomed Mater Res A 71 2004 108 117 (Pubitemid 39244401)
22. J.P. Gleeson, N.A. Plunkett, and F.J. O'Brien Addition of hydroxyapatite improves stiffness, interconnectivity and osteogenic potential of a highly porous collagen-based scaffold for bone tissue regeneration Eur Cell Mater 20 2010 218 230
23. D. Wahl, and J. Czernuszka Collagen-hydroxyapatite composites for hard tissue repair Eur Cell Mater 11 2006 43 56 (Pubitemid 43824730)
24. D. Wendt, M. Jakob, and I. Martin Bioreactor-based engineering of osteochondral grafts: from model systems to tissue manufacturing Journal of Bioscience and Bioengineering 100 2005 489 494 (Pubitemid 43181248)
25. C. Guoping, D. Akahane, N. Kawazoe, K. Yamamoto, and T. Tateishi Chondrogenic differentiation of mesenchymal stem cells in a leakproof collagen sponge Mater Sci Eng C (Biomimetic Supramolecular Syst) 2008 195 201 (Pubitemid 350297874)