DNA extracted from stained sputum smears can be used in the MTBDRplus assay

Journal of Clinical Microbiology

Volumn 49, Issue 10, 2011, Pages 3600-3603

  Cauwelaert, Natasha Dubois ^a   Ramarokoto, Herimanana ^a   Ravololonandriana, Pascaline ^a   Richard, Vincent ^a   Rasolofo, Voahangy ^a  

^a INSTITUT PASTEUR DE MADAGASCAR   (Madagascar)

Author keywords

[No Author keywords available]

Indexed keywords

ISONIAZID; RIFAMPICIN;

ANALYTIC METHOD; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA EXTRACTION; FEASIBILITY STUDY; GENOTYPE; HUMAN; MAJOR CLINICAL STUDY; MULTIDRUG RESISTANT TUBERCULOSIS; MULTIDRUG RESISTANT TUBERCULOSIS PLUS ASSAY; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SPECIES IDENTIFICATION; SPUTUM SMEAR; STAINING; STORAGE TEMPERATURE;

ANTITUBERCULAR AGENTS; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; HUMANS; ISONIAZID; MICROBIAL SENSITIVITY TESTS; MOLECULAR DIAGNOSTIC TECHNIQUES; MYCOBACTERIUM TUBERCULOSIS; RIFAMPIN; SENSITIVITY AND SPECIFICITY; SPECIMEN HANDLING; SPUTUM;

MYCOBACTERIUM TUBERCULOSIS;

EID: 80053533360     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00745-11     Document Type: Article

References (25)

1. Akpaka, P. E., S. Baboolal, D. Clarke, L. Francis, and N. Rastogi. 2008. Evaluation of methods for rapid detection of resistance to isoniazid and rifampin in Mycobacterium tuberculosis isolates collected in the Caribbean. J. Clin. Microbiol. 46:3426-3428.
2. Anek-Vorapong, R., et al. 2010. Validation of the GenoType MTBDRplus assay for detection of MDR-TB in a public health laboratory in Thailand. BMC Infect. Dis. 10:123.
3. Barnard, M., H. Albert, G. Coetzee, R. O'Brien, and M. E. Bosman. 2008. Rapid molecular screening for multidrug-resistant tuberculosis in a highvolume public health laboratory in South Africa. Am. J. Respir. Crit. Care Med. 177:787-792. (Pubitemid 351490566)
4. Canetti, G., N. Rist, and J. Grosset. 1963. Measurement of sensitivity of the tuberculous bacillus to antibacillary drugs by the method of proportions. Methodology, resistance criteria, results and interpretation. Rev. Tuberc. Pneumol. 27:217-272. (In French.)
5. Causse, M., P. Ruiz, J. B. Gutierrez, J. Zerolo, and M. Casal. 2008. Evaluation of new GenoType MTBDRplus for detection of resistance in cultures and direct specimens of Mycobacterium tuberculosis. Int. J. Tuberc. Lung Dis. 12:1456-1460.
6. Evans, J., M. C. Stead, M. P. Nicol, and H. Segal. 2009. Rapid genotypic assays to identify drug-resistant Mycobacterium tuberculosis in South Africa. J. Antimicrob. Chemother. 63:11-16.
7. Gori, A., et al. 2005. Spoligotyping and Mycobacterium tuberculosis. Emerg. Infect. Dis. 11:1242-1248. (Pubitemid 41045333)
8. Hillemann, D., S. Rusch-Gerdes, and E. Richter. 2007. Evaluation of the GenoType MTBDRplus assay for rifampin and isoniazid susceptibility testing of Mycobacterium tuberculosis strains and clinical specimens. J. Clin. Microbiol. 45:2635-2640. (Pubitemid 47295497)
9. Hillemann, D., S. Rusch-Gerdes, and E. Richter. 2009. Feasibility of the GenoType MTBDRsl assay for fluoroquinolone, amikacin-capreomycin, and ethambutol resistance testing of Mycobacterium tuberculosis strains and clinical specimens. J. Clin. Microbiol. 47:1767-1772.
10. Huang, W. L., H. Y. Chen, Y. M. Kuo, and R. Jou. 2009. Performance assessment of the GenoType MTBDRplus test and DNA sequencing in detection of multidrug-resistant Mycobacterium tuberculosis. J. Clin. Microbiol. 47:2520-2524.
11. Huyen, M. N., et al. 2010. Validation of the GenoType MTBDRplus assay for diagnosis of multidrug resistant tuberculosis in South Vietnam. BMC Infect. Dis. 10:149.
12. Kubica, G. P., W. E. Dye, M. L. Cohn, and G. Middlebrook. 1963. Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria. Am. Rev. Respir. Dis. 87:775-779.
13. Lacoma, A., et al. 2008. GenoType MTBDRplus assay for molecular detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis strains and clinical samples. J. Clin. Microbiol. 46:3660-3667.
14. Minime-Lingoupou, F., et al. 2010. Rapid identification of multidrug-resistant tuberculosis isolates in treatment failure or relapse patients in Bangui, Central African Republic. Int. J. Tuberc. Lung Dis. 14:782-785.
15. Miotto, P., et al. 2009. Molecular detection of rifampin and isoniazid resistance to guide chronic TB patient management in Burkina Faso. BMC Infect. Dis. 9:142.
16. Mitchison, D. A. 1984. Drug resistance in mycobacteria. Br. Med. Bull. 40:84-90. (Pubitemid 14219022)
17. Nikolayevskyy, V., et al. 2009. Performance of the Genotype MTBDRPlus assay in the diagnosis of tuberculosis and drug resistance in Samara, Russian Federation. BMC Clin. Pathol. 9:2.
18. Rieder, H. L., et al. 2008. Examen microscopique des frottis de crachats, p. 14-33. In Priorités pour les Services de Bactériologie de la Tuberculose dans les Pays à Faibles Revenus. International Union against Tuberculosis and Lung Disease, Paris, France.
19. Silva, M. R., et al. 2011. Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl Neelsen-stained sputum smears in Brazil. Mem. Inst. Oswaldo Cruz 106:9-15.
20. Somoskovi, A., L. M. Parsons, and M. Salfinger. 2001. The molecular basis of resistance to isoniazid, rifampin, and pyrazinamide in Mycobacterium tuberculosis. Respir. Res. 2:164-168. (Pubitemid 34187923)
21. Suresh, N., J. Arora, H. Pant, T. Rana, and U. B. Singh. 2007. Spoligotyping of Mycobacterium tuberculosis DNA from archival Ziehl-Neelsen-stained sputum smears. J. Microbiol. Methods 68:291-295. (Pubitemid 46149940)
22. Suresh, N., et al. 2007. Rapid detection of rifampin-resistant Mycobacterium tuberculosis directly from stained sputum smears using single-tube nested polymerase chain reaction deoxyribonucleic acid sequencing. Diagn. Microbiol. Infect. Dis. 58:217-222. (Pubitemid 46856814)
23. Van der Zanden, A. G., et al. 1998. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis complex in paraffin wax embedded tissues and in stained microscopic preparations. Mol. Pathol. 51:209-214.
24. Van Der Zanden, A. G., E. M. Te Koppele-Vije, N. Vijaya Bhanu, D. Van Soolingen, and L. M. Schouls. 2003. Use of DNA extracts from Ziehl-Neelsen-stained slides for molecular detection of rifampin resistance and spoligotyping of Mycobacterium tuberculosis. J. Clin. Microbiol. 41:1101-1108. (Pubitemid 36307113)
25. World Health Organization. 2008. Policy statement: molecular line probe assays for rapid screening of patients at risk of multidrug-resistant tuberculosis (MDR-TB). World Health Organization, Geneva, Switzerland.