Site-specific labeling of proteins for single-molecule fret measurements using genetically encoded ketone functionalities

Methods in Molecular Biology

Volumn 751, Issue , 2011, Pages 3-15

  Lemke, Edward A ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

Amber stop codon; FRET; Genetically encoded; Maleimide coupling; Oxime ligation; Protein dynamics; Single molecule; Site specific labeling; Synthetase; Unnatural amino acid

Indexed keywords

AFALANINE; AMINO ACID; CYSTEINE; FLUORESCENT DYE; HYDROXYLAMINE; KETONE; MALEIMIDE; PROTEIN; DRUG DERIVATIVE; LYSOZYME; MALEIMIDE DERIVATIVE; N-ACETYLPHENYLALANINE; OXIME; PHENYLALANINE; RECOMBINANT PROTEIN;

CHEMISTRY; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; MEASUREMENT; NONHUMAN; PROTEIN EXPRESSION; PROTEIN STRUCTURE; STOP CODON; ARTICLE; BACTERIOPHAGE T4; BINDING SITE; ENZYMOLOGY; GENETIC TRANSFORMATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; STAINING;

BACTERIOPHAGE T4; BINDING SITES; CYSTEINE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; HYDROXYLAMINE; KETONES; MALEIMIDES; MURAMIDASE; OXIMES; PHENYLALANINE; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; STAINING AND LABELING; TRANSFORMATION, GENETIC;

EID: 80053305452     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-151-2_1     Document Type: Chapter

References (6)

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