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Perform Southern blot analysis (18) using the entire knockout cassette or the hph gene as a probe. The DIG-labeled DNA molecular weight marker VII is run with samples on the agarose gel. The PCR DIG probe synthesis kit is used for probe amplification and labeling, while the DIG Easy Hyb, DIG wash and block buffer set, Anti-digoxigenin-AP conjugate, and CDP-Star are used for hybridization and detection. Colot, H. V., Park, G., Collopy, P., Ringelberg, C., Crew, C., Litvinkova, L., DeCaprio, D., Hood, H. M., Curilla, S., Shi, M., Crawford, M., Koerhsen, M., Montgomery, P., Larson, L., Pearson, M., Kasuga, T., Tian, C., Basturkmen, M., Altamirano, L., and Xu, J. (2007) Enabling a community to dissect an organism: overview of the Neurospora functional genomics project. Adv Genet 57, 49–96.
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Perform Southern blot analysis (18) using the entire knockout cassette or the hph gene as a probe. The DIG-labeled DNA molecular weight marker VII is run with samples on the agarose gel. The PCR DIG probe synthesis kit is used for probe amplification and labeling, while the DIG Easy Hyb, DIG wash and block buffer set, Anti-digoxigenin-AP conjugate, and CDP-Star are used for hybridization and detection. Colot, H. V., Park, G., Collopy, P., Ringelberg, C., Crew, C., Litvinkova, L., DeCaprio, D., Hood, H. M., Curilla, S., Shi, M., Crawford, M., Koerhsen, M., Montgomery, P., Larson, L., Pearson, M., Kasuga, T., Tian, C., Basturkmen, M., Altamirano, L., and Xu, J. (2007) Enabling a community to dissect an organism: overview of the Neurospora functional genomics project. Adv Genet 57, 49–96.
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