Utilization of non-AUG initiation codons in a flow cytometric method for efficient selection of recombinant cell lines

Biotechnology and Bioengineering

Volumn 108, Issue 11, 2011, Pages 2611-2622

  Cairns, Victor R ^a   Demaria, Christine T ^a   Poulin, Francis ^a   Sancho, José ^a   Liu, Ping ^a   Zhang, Jin ^a   Campos Rivera, Juanita ^a   Karey, Kenneth P ^a   Estes, Scott ^a  

^a GENZYME CORPORATION   (United States)

Author keywords

CHO cells; Clone selection; FACS; Flow cytometry; Protein expression; Translation

Indexed keywords

CELL POPULATIONS; CELL SURFACES; CHO CELL; CLONE SELECTION; FACS; INITIATION EFFICIENCY; OPEN READING FRAME; PROTEIN EXPRESSION; PROTEIN EXPRESSIONS; RECOMBINANT CELLS; RECOMBINANT PROTEIN; REPORTER PROTEIN; SCREENING TOOL; STABLE EXPRESSION;

CELL CULTURE; CELL MEMBRANES; CELL PROLIFERATION; CLONE CELLS; CLONING; FLOW CYTOMETRY; GENE EXPRESSION; LAKES; TRANSLATION (LANGUAGES);

PROTEINS;

ADENINE; GUANINE; MESSENGER RNA; RECOMBINANT PROTEIN; URIDINE;

ANIMAL CELL; ARTICLE; CELL CLONE; CELL LINE; CELL POPULATION; CELL SELECTION; CHO CELL; FLOW CYTOMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; REPORTER GENE; START CODON; TRANSLATION INITIATION;

ANIMALS; CELL CULTURE TECHNIQUES; CHO CELLS; CODON, INITIATOR; CRICETINAE; CRICETULUS; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GENES, REPORTER; MASS SCREENING; RECOMBINANT PROTEINS;

EID: 80053053445     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.23219     Document Type: Article

References (25)

1. Bailey CG, Tait AS, Sunstrom NA. 2002. High-throughput clonal selection of recombinant CHO cells using a dominant selectable and amplifiable metallothionein-GFP fusion protein. Biotechnol Bioeng 80(6): 670-676.
2. Bochkov YA, Palmenberg AC. 2006. Translational efficiency of EMCV IRES in bicistronic vectors is dependent upon IRES sequence and gene location. Biotechniques 41(3): 283-284 (286, 288 passim).
3. Bohm E, Grillari J, Voglauer R, Gross S, Ernst W, Ferko B, Kunert R, Katinger H, Borth N. 2005. Establishment of a strategy for the rapid generation of a monoclonal antibody against the human protein SNEV (hNMP200) by flow-cytometric cell sorting. J Immunol Methods 307(1-2): 13-23.
4. Brezinsky SC, Chiang GG, Szilvasi A, Mohan S, Shapiro RI, MacLean A, Sisk W, Thill G. 2003. A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity. J Immunol Methods 277(1-2): 141-155.
5. Browne SM, Al-Rubeai M. 2007. Selection methods for high-producing mammalian cell lines. Trends Biotechnol 25(9): 425-432.
6. Caron AW, Nicolas C, Gaillet B, Ba I, Pinard M, Garnier A, Massie B, Gilbert R. 2009. Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins. BMC Biotechnol 9:42.
7. DeMaria CT, Cairns V, Schwarz C, Zhang J, Guerin M, Zuena E, Estes S, Karey KP. 2007. Accelerated clone selection for recombinant CHO CELLS using a FACS-based high-throughput screen. Biotechnol Prog 23(2): 465-472.
8. Gaillet B, Gilbert R, Broussau S, Pilotte A, Malenfant F, Mullick A, Garnier A, Massie B. 2010. High-level recombinant protein production in CHO cells using lentiviral vectors and the cumate gene-switch. Biotechnol Bioeng 106(2): 203-215.
9. Gaines P, Wojchowski DM. 1999. pIRES-CD4t, a dicistronic expression vector for MACS- or FACS-based selection of transfected cells. Biotechniques 26(4): 683-688.
10. Jackson RJ. 1996. A comparative view of initiation site selection mechanisms. In: Hershey JWB, Mathews MB, Sonenberg N, editors. Translation Control. Cold Spring Harbor: Cold Spring Harbor Laboratory Press. p. 71-112.
11. Kozak M. 1989. Context effects and inefficient initiation at non-AUG codons in eucaryotic cell-free translation systems. Mol Cell Biol 9(11): 5073-5080.
12. Kozak M. 1990. Downstream secondary structure facilitates recognition of initiator codons by eukaryotic ribosomes. Proc Natl Acad Sci USA 87(21): 8301-8305.
13. Kozak M. 2005. A second look at cellular mRNA sequences said to function as internal ribosome entry sites. Nucleic Acids Res 33(20): 6593-6602.
14. Liu X, Constantinescu SN, Sun Y, Bogan JS, Hirsch D, Weinberg RA, Lodish HF. 2000. Generation of mammalian cells stably expressing multiple genes at predetermined levels. Anal Biochem 280(1): 20-28.
15. Loken MR, Green CL, Wells DA. 2000. Immunofluorescence of surface markers. In: Hames BD, editor. Flow Cytometry. 3rd edn. Oxford, UK: Oxford University Press. p. 61-82.
16. Mehdi H, Ono E, Gupta KC. 1990. Initiation of translation at CUG, GUG, and ACG codons in mammalian cells. Gene 91(2): 173-178.
17. Peabody DS. 1989. Translation initiation at non-AUG triplets in mammalian cells. J Biol Chem 264(9): 5031-5035.
18. Pestova TA, Lorsch JR, Hellen CUT. 2007. The mechanism of translation initiation in eukarotes. In: Mathews MB, Sonenberg N, Hershey WJ, editors. Translational control in biology and medicine. Cold Spring Harbor: Cold Spring Harbor Laboratory Press. p. 87-128.
19. Prentice HL, Tonkin CJ, Caamano L, Sisk WP. 2007. High level expression of proteins using sequences from the ferritin heavy chain gene locus. J Biotechnol 128(1): 50-60.
20. Running Deer J, Allison DS. 2004. High-level expression of proteins in mammalian cells using transcription regulatory sequences from the Chinese hamster EF-1alpha gene. Biotechnol Prog 20(3): 880-889.
21. Sautter K, Enenkel B. 2005. Selection of high-producing CHO cells using NPT selection marker with reduced enzyme activity. Biotechnol Bioeng 89(5): 530-538.
22. Serpieri F, Inocencio A, de Oliveira JM, Pimenta AA Jr., Garbuio A, Kalil J, Brigido MM, Moro AM. 2010. Comparison of humanized IgG and FvFc anti-CD3 monoclonal antibodies expressed in CHO cells. Mol Biotechnol 45(3): 218-225.
23. Sleiman RJ, Gray PP, McCall MN, Codamo J, Sunstrom NA. 2008. Accelerated cell line development using two-color fluorescence activated cell sorting to select highly expressing antibody-producing clones. Biotechnol Bioeng 99(3): 578-587.
24. van Blokland HJ, Kwaks TH, Sewalt RG, Verhees JA, Klaren VN, Siersma TK, Korse JW, Teunissen NC, Botschuijver S, van Mer C, et al. 2007. A novel, high stringency selection system allows screening of few clones for high protein expression. J Biotechnol 128(2): 237-245.
25. Xia W, Bringmann P, McClary J, Jones PP, Manzana W, Zhu Y, Wang S, Liu Y, Harvey S, Madlansacay MR, et al. 2006. High levels of protein expression using different mammalian CMV promoters in several cell lines. Protein Expr Purif 45(1): 115-124.