Structure-guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells

Biochemistry

Volumn 50, Issue 38, 2011, Pages 8221-8225

  Cohen, Justin D ^a   Thompson, Samuel ^a   Ting, Alice Y ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

7-HYDROXYCOUMARIN; BLUE FLUOROPHORE; CELL SURFACES; DOUBLE MUTANTS; E. COLI; ENDOSOMES; ISOSTERIC; LIPOIC ACIDS; LIVING CELL; MATRIX; PROTEIN IMAGING; RECOMBINANT PROTEIN; SUBSTRATE SPECIFICITY;

AMINO ACIDS; CELL MEMBRANES; ESCHERICHIA COLI; PROTEINS; SUBSTRATES;

FLUOROPHORES;

ANION; LIGASE; LIPOIC ACID LIGASE; PACIFIC BLUE LIGASE; THIOCTIC ACID; TRYPTOPHAN; UMBELLIFERONE; UNCLASSIFIED DRUG;

ARTICLE; BIOENGINEERING; CELL LABELING; CELL SURFACE; ENDOSOME; ENZYME SPECIFICITY; HUMAN; HUMAN CELL; IMAGING SYSTEM; MUTATIONAL ANALYSIS; PRIORITY JOURNAL; SCREENING TEST; STRUCTURE ANALYSIS;

AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; ESCHERICHIA COLI PROTEINS; FLUORESCENT DYES; HEK293 CELLS; HELA CELLS; HUMANS; LIGASES; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

PYRONIA TITHONUS;

EID: 80053028232     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi201037r     Document Type: Article

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