Using luciferase to image bacterial infections in mice

Journal of Visualized Experiments

Volumn , Issue 48, 2010, Pages

  Chang, Mi Hee ^a   Cirillo, Suat L G ^a   Cirillo, Jeffrey D ^a  

^a TEXAS A AND M HEALTH SCIENCE CENTER   (United States)

Author keywords

Bioluminescence; Click beetle; Firefly; In vivo imaging; Infection; Issue 48; Pathogenesis; Whole animal imaging

Indexed keywords

EID: 80053023474     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2547     Document Type: Article

References (12)

1. Wilson, T. & Hastings, J. W. Bioluminescence. Annu Rev Cell Dev Biol 14, 197-230, doi:10.1146/annurev.cellbio.14.1.197 (1998).
2. Contag, C. H. & Bachmann, M. H. Advances in in vivo bioluminescence imaging of gene expression. Annu Rev Biomed Eng 4, 235-260, doi:10.1146/annurev.bioeng.4.111901.093336111901.093336 [pii] (2002).
3. Hutchens, M. & Luker, G. D. Applications of bioluminescence imaging to the study of infectious diseases. Cell Microbiol 9, 2315-2322, doi:CMI995 [pii] 10.1111/j.1462-5822.2007.00995.x (2007).
4. Doyle, T. C., Burns, S. M. & Contag, C. H. In vivo bioluminescence imaging for integrated studies of infection. Cell Microbiol 6, 303-317, doi:378 [pii] (2004).
5. Wood, K. V., Lam, Y. A., Seliger, H. H. & McElroy, W. D. Complementary DNA coding click beetle luciferases can elicit bioluminescence of different colors. Science 244, 700-702 (1989).
6. de Wet, J. R., Wood, K. V., Helinski, D. R. & DeLuca, M. Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc Natl Acad Sci U S A 82, 7870-7873 (1985).
7. Hastings, J. W. Chemistries and colors of bioluminescent reactions: a review. Gene 173, 5-11, doi:0378111995006761 [pii] (1996).
8. Zhao, H. et al. Emission spectra of bioluminescent reporters and interaction with mammalian tissue determine the sensitivity of detection in vivo. J Biomed Opt 10, 41210, doi:10.1117/1.2032388 (2005).
9. Rice, B. W., Cable, M. D. & Nelson, M. B. In vivo imaging of light-emitting probes. J Biomed Opt 6, 432-440, doi:10.1117/1.1413210 (2001).
10. Contag, C. H. et al. Photonic detection of bacterial pathogens in living hosts. Mol Microbiol 18, 593-603 (1995).
11. Kuo, C., Coquoz, O., Troy, T. L., Xu, H. & Rice, B. W. Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging. J Biomed Opt 12, 024007, doi:10.1117/1.2717898 (2007).
12. Weissleder, R. A clearer vision for in vivo imaging. Nat Biotechnol 19, 316-317, doi:10.1038/86684 86684 [pii] (2001).