A portable microfluidic fluorescence spectrometer device for γ-H2AX-based biological dosimetry

Radiation Measurements

Volumn 46, Issue 9, 2011, Pages 907-911

  Pope, I A ^a   Barber, P R ^a   Horn, S ^b   Ainsbury, E ^b   Rothkamm, K ^b   Vojnovic, B ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b PUBLIC HEALTH ENGLAND   (United Kingdom)

Author keywords

Flow cytometry; Fluorescence spectroscopy; Gamma H2AX; Ionizing radiation; Microfluidics; Post accident dosimetry

Indexed keywords

BIOLOGICAL DOSIMETRY; BLOOD SAMPLES; DOUBLE-STRAND BREAKS; EMISSION SPECTRUMS; FLUORESCENCE SPECTROMETERS; GAMMA-H2AX; GOLD STANDARDS; HIGH THROUGHPUT; LYMPHOBLASTOID CELLS; MEDICAL TREATMENT; MICROFLUIDIC CHAMBERS; PHOSPHORYLATED HISTONE; PORTABLE DEVICE; PROOF OF PRINCIPLES; RADIATION EXPOSURE; RADIATION-INDUCED; RAPID IDENTIFICATION; X-RAY DOSE;

DOSIMETRY; EMISSION SPECTROSCOPY; FLOW CYTOMETRY; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; IONIZING RADIATION; PHOSPHORYLATION; RADIOLOGY; SPECTROMETERS; SPECTROMETRY;

MICROFLUIDICS;

EID: 80052958787     PISSN: 13504487     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.radmeas.2011.02.004     Document Type: Conference Paper

References (15)

1. A. Andrievski, and R.C. Wilkins The response of gamma-H2AX in human lymphocytes and lymphocytes subsets measured in whole blood cultures Int. J. Radiat. Biol. 85 2009 369 376
2. P.R. Barber, B. Vojnovic, G. Atkin, F.M. Daley, S.A. Everett, G.D. Wilson, and J.D. Gilbey Applications of cost-effective spectral imaging microscopy in cancer research J. Phys. D: Appl. Phys. 36 2003 1729
3. G. Garty, Y. Chen, A. Salerno, H. Turner, J. Zhang, O. Lyulko, A. Bertucci, Y. Xu, H. Wang, N. Simaan, G. Randers-Pehrson, Y.L. Yao, S.A. Amundson, and D.J. Brenner The RABIT: a rapid automated biodosimetry tool for radiological triage Health Phys. 98 2010 209 217
4. K. Hamasaki, K. Imai, K. Nakachi, N. Takahashi, Y. Kodama, and Y. Kusunoki Short-term culture and gammaH2AX flow cytometry determine differences in individual radiosensitivity in human peripheral T lymphocytes Environ. Mol. Mutagen 48 2007 38 47 (Pubitemid 46238557)
5. Horn, S.; Rothkamm, K.;. Candidate protein biomarkers as rapid indicators of radiation exposure. Radiat. Meas.; this issue, in revision.
6. X. Huang, H.D. Halicka, F. Traganos, T. Tanaka, A. Kurose, and Z. Darzynkiewicz Cytometric assessment of DNA damage in relation to cell cycle phase and apoptosis Cell Prolif. 38 2005 223 243 (Pubitemid 41176576)
7. International Atomic Energy Agency Cytogenetic Analysis for Radiation Dose Assessment 2001 A manual. IAEA Technical Report Series 405
8. I.H. Ismail, T.I. Wadhra, and O. Hammarsten An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans Nucleic Acids Res. 35 2007 e36
9. M. Löbrich, N. Rief, M. Kühne, M. Heckmann, J. Fleckenstein, C. Rübe, and M. Uder In vivo formation and repair of DNA double-strand breaks after computed tomography examinations Proc. Natl. Acad. Sci. U.S.A 102 2005 8984 8989 (Pubitemid 40881065)
10. C.E. Redon, A.J. Nakamura, K. Gouliaeva, A. Rahman, W.F. Blakely, and W.M. Bonner The use of gamma-H2AX as a biodosimeter for total-body radiation exposure in non-human primates PLoS One 5 2010 e15544
11. E.P. Rogakou, C. Boon, C. Redon, and W.M. Bonner Megabase chromatin domains involved in DNA double-strand breaks in vivo J. Cell Biol. 146 1999 905 916
12. K. Rothkamm, and M. Löbrich Evidence for a lack of DNA double-strand break repair in human cells exposed to very low x-ray doses Proc. Natl. Acad. Sci. U.S.A. 100 2003 5057 5062 (Pubitemid 36542657)
13. K. Rothkamm, S. Balroop, J. Shekhdar, P. Fernie, and V. Goh Leukocyte DNA damage after multi-detector row CT: a quantitative biomarker of low-level radiation exposure Radiology 242 2007 244 251 (Pubitemid 44956030)
14. O.A. Sedelnikova, E.P. Rogakou, I.G. Panyutin, and W.M. Bonner Quantitative detection of (125)IdU-induced DNA double-strand breaks with gamma-H2AX antibody Radiat. Res. 158 2002 486 492
15. H. van Attikum, and S.M. Gasser Crosstalk between histone modifications during the DNA damage response Trends Cell Biol. 19 2009 207 217