Isolation, Quantification, and Analysis of Chloroplast DNA

Methods in Molecular Biology

Volumn 774, Issue , 2011, Pages 151-170

  Rowan, Beth A ^a   Bendich, Arnold J ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Chloroplast DNA; DNA fluorophore; Flow cytometry; Fluorescence microscopy; Quantitative PCR

Indexed keywords

ARABIDOPSIS; ARABIDOPSIS THALIANA;

4',6 DIAMIDINO 2 PHENYLINDOLE; ALKALINE PHOSPHATASE; CETRIMIDE; CHLOROPLAST DNA; ETHIDIUM; FLUORESCENT DYE; INDOLE DERIVATIVE;

ARABIDOPSIS; ARTICLE; CELL NUCLEUS; CHLOROPLAST; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR BIOLOGY; NUCLEIC ACID HYBRIDIZATION; PULSED FIELD GEL ELECTROPHORESIS; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAINING;

ALKALINE PHOSPHATASE; ARABIDOPSIS; CELL NUCLEUS; CETRIMONIUM COMPOUNDS; CHLOROPLASTS; DNA, CHLOROPLAST; ELECTROPHORESIS, GEL, PULSED-FIELD; ETHIDIUM; FLOW CYTOMETRY; FLUORESCENT DYES; INDOLES; MICROSCOPY, FLUORESCENCE; MOLECULAR BIOLOGY; NUCLEIC ACID HYBRIDIZATION; REAL-TIME POLYMERASE CHAIN REACTION; RESTRICTION MAPPING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; STAINING AND LABELING;

EID: 80052917300     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-234-2_10     Document Type: Chapter

References (10)

1. Lopez-Juez E., and Pyke K. A. (2005) Plastids unleashed: their development and their integration in plant development. Int. J. Dev. Biol. 49, 557–577.
2. Waters M. T., and Langdale J. A. (2009) The making of a chloroplast. EMBO J. 28, 2861–2873.
3. Fujie M., Kuroiwa H., Kawano S., Mutoh S., and Kuroiwa T. (1994) Behavior of organelles and their nucleoids in the shoot apical meristem during leaf development in Arabidopsis thaliana L. Planta 194, 395–405.
4. Kuroiwa T, Suszuki T, Ogawa K, and Kawano S. (1981) The chloroplast nucleus: distribution, number, size, and shape, and a model for the multiplication of the chloroplast genome during chloroplast development. Plant Cell Physiol. 22, 381–396.
5. Oldenburg D. J., and Bendich A. J. (2004) Changes in the structure of DNA molecules and the amount of DNA per plastid during chloroplast development in maize. J. Mol. Biol. 344, 1311–1330.
6. Rowan B. A., Oldenburg D. J., and Bendich A. J. (2004) The demise of chloroplast DNA in Arabidopsis. Curr. Genet. 46, 176–181.
7. Rowan B. A., Oldenburg D. J., and Bendich A. J. (2009) A multiple-method approach reveals a declining amount of chloroplast DNA during development in Arabidopsis. BMC Plant Biol. 9, 3.
8. Oldenburg D. J., and Bendich A. J. (2009) Chloroplasts. In, Molecular Genetic Approaches to Maize Improvement (Kriz A. L., and Larkins B. A., eds.) Springer-Verlag, Heidelberg, Germany, pp. 325–343.
9. Oldenburg D. J., Rowan B. A., Zhao L., Walcher C. L., Schleh M., and Bendich A. J. (2006) Loss or retention of chloroplast DNA in maize seedlings is affected by both light and genotype. Planta 225, 41–55.
10. Shaver J. M., Oldenburg D. J., and Bendich A. J. (2008) The structure of chloroplast DNA molecules and the effects of light on the amount of chloroplast DNA during development in Medicago truncatula. Plant Physiol. 146, 1064–1074.