Diagnosis of congenital toxoplasmosis by polymerase chain reaction on neonatal peripheral blood

Diagnostic Microbiology and Infectious Disease

Volumn 71, Issue 2, 2011, Pages 174-176

  Sterkers, Yvon ^a,b,c   Ribot, Jennifer ^a,b,c   Albaba, Sahar ^a,b,c   Issert, Eric ^a,b,c   Bastien, Patrick ^a,b,c   Pratlong, Francine ^a,b,c  

^a MONTPELLIER UNIVERSITY HOSPITAL   (France)

^b The Pole Molecular Biology of the National Reference Centre of Toxoplasmosis   (France)

^c HÔPITAL ARNAUD DE VILLENEUVE   (France)

Author keywords

Amniotic fluid; Cord blood; PCR; Placenta; Toxoplasma gondii

Indexed keywords

ARTICLE; BLOOD ANALYSIS; CLINICAL ARTICLE; COHORT ANALYSIS; CONGENITAL TOXOPLASMOSIS; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; EARLY DIAGNOSIS; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; FOLLOW UP; HUMAN; MOLECULAR DIAGNOSIS; NEWBORN; OUTCOME ASSESSMENT; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROSPECTIVE STUDY; SENSITIVITY ANALYSIS; SERODIAGNOSIS; VALIDATION PROCESS;

FEMALE; FOLLOW-UP STUDIES; HUMANS; INFANT, NEWBORN; INFECTIOUS DISEASE TRANSMISSION, VERTICAL; POLYMERASE CHAIN REACTION; PREGNANCY; PREGNANCY COMPLICATIONS, PARASITIC; PROSPECTIVE STUDIES; TOXOPLASMA; TOXOPLASMOSIS, CONGENITAL;

EID: 80052861090     PISSN: 07328893     EISSN: 18790070     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2011.06.006     Document Type: Article

References (13)

1. Bastien P. Molecular diagnosis of toxoplasmosis. Trans. R. Soc. Trop. Med. Hyg. 2002, 96(Suppl 1):S205-S215.
2. Bastien P., Jumas-Bilak E., Varlet-Marie E., Marty P. Three years of multi-laboratory external quality control for the molecular detection of Toxoplasma gondii in amniotic fluid in France. Clin. Microbiol. Infect. 2007, 13:430-433.
3. Bergstrom T., Ricksten A., Nenonen N., Lichtenstein M., Olofsson S. Congenital Toxoplasma gondii infection diagnosed by PCR amplification of peripheral mononuclear blood cells from a child and mother. Scand. J. Infect. Dis. 1998, 30:202-204.
4. Bretagne S., Costa J.M., Vidaud M., Tran J., Nhieu V., Fleury-Feith J. Detection of Toxoplasma gondii by competitive DNA amplification of bronchoalveolar lavage samples. J. Infect. Dis. 1993, 168:1585-1588.
5. Chabbert E., Lachaud L., Crobu L., Bastien P. Comparison of two widely used PCR primer systems for detection of toxoplasma in amniotic fluid, blood, and tissues. J. Clin. Microbiol. 2004, 42:1719-1722.
6. Desmonts G., Couvreur J. Toxoplasmosis in pregnancy and its transmission to the fetus. Bull. N. Y. Acad. Med. 1974, 50:146-159.
7. King L., Villena I., Ancelle T., Wallon M., Garcia P., Thulliez P., Goulet V. Congenital toxoplasmosis: implementation of a surveillance system in France. Bull. Epidémiologique. Hebdomadaire. 2008, 14-15:122-123.
8. Lebech M., Joynson D.H., Seitz H.M., Thulliez P., Gilbert R.E., Dutton G.N., Ovlisen B., Petersen E. Classification system and case definitions of Toxoplasma gondii infection in immunocompetent pregnant women and their congenitally infected offspring. European Research Network on Congenital Toxoplasmosis. Eur. J. Clin. Microbiol. Infect. Dis. 1996, 15:799-805.
9. Pratlong F., Boulot P., Villena I., Issert E., Tamby I., Cazenave J., Dedet J.P. Antenatal diagnosis of congenital toxoplasmosis: evaluation of the biological parameters in a cohort of 286 patients. Br. J. Obstet. Gynaecol. 1996, 103:552-557.
10. Robert-Gangneux F., Commerce V., Tourte-Schaefer C., Dupouy-Camet J. Performance of a Western blot assay to compare mother and newborn anti-Toxoplasma antibodies for the early neonatal diagnosis of congenital toxoplasmosis. Eur. J. Clin. Microbiol. Infect. Dis. 1999, 18:648-654.
11. Sterkers Y., Varlet-Marie E., Cassaing S., Brenier-Pinchart M.P., Brun S., Dalle F., Delhaes L., Filisetti D., Pelloux H., Yera H., Bastien P. Multicentric comparative analytical performance study for molecular detection of low amounts of Toxoplasma gondii from simulated specimens. J. Clin. Microbiol. 2010, 48:3216-3222.
12. Sterkers Y., Varlet-Marie E., Marty P., Bastien P. Diversity and evolution of methods and practices for the molecular diagnosis of congenital toxoplasmosis in France: a 4-year survey. Clin. Microbiol. Infect. 2010, 16:1594-1602. ANOFEL Toxoplasma-PCR Quality Control Group.
13. Tissot Dupont D., Fricker-Hidalgo H., Brenier-Pinchart M.P., Bost-Bru C., Ambroise-Thomas P., Pelloux H. Usefulness of Western blot in serological follow-up of newborns suspected of congenital toxoplasmosis. Eur. J. Clin. Microbiol. Infect. Dis. 2003, 22:122-125.