Molecular-based environmental risk assessment of three varieties of genetically engineered cows

Transgenic Research

Volumn 20, Issue 5, 2011, Pages 1043-1054

  Xu, Jianxiang ^a   Zhao, Jie ^a   Wang, Jianwu ^a   Zhao, Yaofeng ^a   Zhang, Lei ^a   Chu, Mingxing ^b   Li, Ning ^a  

^a CHINA AGRICULTURAL UNIVERSITY   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

16S rDNA sequencing; Environmental risk assessment; Genetically engineered animal; PCR DGGE; Phylogenetic analysis

Indexed keywords

LACTALBUMIN; LACTOFERRIN; LYSOZYME; RNA 16S;

ANIMAL; ARTICLE; CATTLE; ENVIRONMENTAL ASPECTS AND RELATED PHENOMENA; GENETICS; HORIZONTAL GENE TRANSFER; HUMAN; MANURE; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; PHYSIOLOGY; RISK ASSESSMENT; TRANSGENE; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BASE SEQUENCE; CATTLE; ECOLOGICAL AND ENVIRONMENTAL PROCESSES; GENE TRANSFER, HORIZONTAL; HUMANS; LACTALBUMIN; LACTOFERRIN; MANURE; MOLECULAR SEQUENCE DATA; MURAMIDASE; PHYLOGENY; RISK ASSESSMENT; RNA, RIBOSOMAL, 16S; SOIL MICROBIOLOGY; TRANSGENES;

ACIDOBACTERIA; ACTINOBACTERIA; ALPHAPROTEOBACTERIA; ANIMALIA; BACTERIA (MICROORGANISMS); BACTEROIDETES; BETAPROTEOBACTERIA; CHLOROFLEXI; CHLOROFLEXI (CLASS); DELTAPROTEOBACTERIA; EPSILONPROTEOBACTERIA; FIRMICUTES; GAMMAPROTEOBACTERIA; NITROSPIRA; SPIROCHAETALES; SPIROCHAETES;

EID: 80052860689     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-010-9477-3     Document Type: Article

References (56)

1. ® salmon. http://www. fda. gov/downloads/AdvisoryCommittees/CommitteesMeetingMaterials/VeterinaryMedicineAdvisoryCommittee/UCM224760. pdf.
2. Badawy OM, El-Seehy MM, Saadalla MM, Badran MS, Salem ES (2008) Biosafety and risk assessment of transgenic sugarcane plants. Sugar Technol 10(3): 234-242. doi: 10. 1007/s12355-008-0042-9.
3. Bassam BJ, Caetanoanolles G (1993) Silver staining of DNA in polyacrylamide gels. Appl Biochem Biotechnol 42(2-3): 181-188.
4. Bassam BJ, Caetanoanolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196(1): 80-83.
5. Boon N, De Windt W, Verstraete W, Top EM (2002) Evaluation of nested PCR-DGGE (denaturing gradient gel electrophoresis) with group-specific 16s rRNA primers for the analysis of bacterial communities from different wastewater treatment plants. FEMS Microbiol Ecol 39(2): 101-112.
6. Broom DM (1993) Assessing the welfare of modified or treated animals. Livest Prod Sci 36(1): 39-54.
7. Bruggemann EP (1993) Environmental safety issues for genetically-modified animals. J Anim Sci 71: 47-50.
8. Caffin JP, Poutrel B, Rainard P (1985) Physiological and pathological factors influencing bovine alpha-lactalbumin and beta-lactoglobulin concentrations in milk. J Dairy Sci 68(5): 1087-1094. doi: 10. 3168/jds. S0022-0302(85)80933-4.
9. Carrigg C, Rice O, Kavanagh S, Collins G, O'Flaherty V (2007) DNA extraction method affects microbial community profiles from soils and sediment. Appl Microbiol Biotechnol 77(4): 955-964. doi: 10. 1007/s00253-007-1219-y.
10. Chakravorty S, Helb D, Burday M, Connell N, Alland D (2007) A detailed analysis of 16s ribosomal RNA gene segments for the diagnosis of pathogenic bacteria. J Microbiol Methods 69(2): 330-339. doi: 10. 1016/j. mimet. 2007. 02. 005.
11. Chandan RC, Shahani KM, Holly RG (1964) Lysozyme content of human milk. Nature 204: 76-77.
12. Cheng JB, Wang JQ, Bu DP, Liu GL, Zhang CG, Wei HY, Zhou LY, Wang JZ (2008) Factors affecting the lactoferrin concentration in bovine milk. J Dairy Sci 91(3): 970-976. doi: 10. 3168/jds. 2007-0689.
13. Clegg CD, Lovell RDL, Hobbs PJ (2003) The impact of grassland management regime on the community structure of selected bacterial groups in soils. FEMS Microbiol Ecol 43(2): 263-270.
14. Deppenmeier S, Bock O, Mengel M, Niemann H, Kues W, Lemme E, Wirth D, Wonigeit K, Kreipe H (2006) Health status of transgenic pigs expressing the human complement regulatory protein CD59. Xenotransplantation 13(4): 345-356. doi: 10. 1111/j. 1399-3089. 2006. 00317. x.
15. Doblhoff-Dier O, Collins CH (2001) Biosafety: future priorities for research in health care. J Biotechnol 85(2): 227-239.
16. Dolezel M, Miklau M, Eckerstorfer M, Hilbeck A, Heissenberger A, Gaugitsch H (2009) Standardising the environmental risk assessment of genetically modified plants in the eu. BfN-Skripten (Bundesamt fur Naturschutz) (259): 295.
17. Dröge M, Pühler A, Selbitschka W (1998) Horizontal gene transfer as a biosafety issue: a natural phenomenon of public concern. J Biotechnol 64(1): 75-90. doi: 10. 1016/S0168-1656(98)00105-9.
18. Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, Gill SR, Nelson KE, Relman DA (2005) Diversity of the human intestinal microbial flora. Science 308(5728): 1635-1638. doi: 10. 1126/science. 1110591.
19. Farthing MJG (2004) Bugs and the gut: an unstable marriage. Best Pract Res Clin Gastroenterol 18(2): 233-239. doi: 10. 1016/j. bpg. 2003. 11. 001.
20. Ferris MJ, Ward DM (1997) Seasonal distributions of dominant 16s rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis. Appl Environ Microbiol 63(4): 1375-1381.
21. Jackson JG, Janszen DB, Lonnerdal B, Lien EL, Pramuk KP, Kuhlman CF (2004) A multinational study of alpha-lactalbumin concentrations in human milk. J Nutr Biochem 15(9): 517-521. doi: 10. 1016/j. jnutbio. 2003. 10. 009.
22. Jackson KA, Berg JM, Murray JD, Maga EA (2010) Evaluating the fitness of human lysozyme transgenic dairy goats: growth and reproductive traits. Transgenic Res. doi: 10. 1007/s11248-010-9371-z.
23. Jensen MA, Straus N (1993) Effect of pcr conditions on the formation of heteroduplex and single-stranded DNA products in the amplification of bacterial ribosomal DNA spacer regions. Genome Res 3: 186-194.
24. Johnson KL, Raybould AF, Hudson MD, Poppy GM (2007) How does scientific risk assessment of gm crops fit within the wider risk analysis? Trends Plant Sci 12(1): 1-5.
25. Kay E, Vogel TM, Bertolla F, Nalin R, Simonet P (2002) In situ transfer of antibiotic resistance genes from transgenic (transplastomic) tobacco plants to bacteria. Appl Environ Microbiol 68(7): 3345-3351.
26. Kelly BG, Vespermann A, Bolton DJ (2009) Gene transfer events and their occurrence in selected environments. Food Chem Toxicol 47(5): 978-983.
27. Kidwell MG (1993) Lateral transfer in natural populations of eukaryotes. Annu Rev Genet 27(1): 235-256. doi: 10. 1146/annurev. ge. 27. 120193. 001315.
28. Kim M-C, Ahn J-H, Shin H-C, Kim T, Ryu T-H, Kim D-H, Song H-G, Lee GH, Ka J-O (2008) Molecular analysis of bacterial community structures in paddy soils for environmental risk assessment with two varieties of genetically modified rice, iksan 483 and milyang 204. J Microbiol Biotechnol 18(2): 207-218.
29. Kocherginskaya SA, Aminov RI, White BA (2001) Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis, random sequencing, and statistical ecology approaches. Anaerobe 7(3): 119-134.
30. Lemaux PG (2008) Genetically engineered plants and foods: a scientist's analysis of the issues (part i). Annu Rev Plant Biol 59: 771-812. doi: 10. 1146/annurev. arplant. 58. 032806. 103840.
31. Liu Z, Zhao C, Fan B, Dai Y, Zhao Z, Wang L, Zheng M, Feng J, Chen Y, Duan Y, Li N (2004) Variable expression of human lactoferrin gene in mice milk driven by its 90 kb upstream flanking sequences. Anim Biotechnol 15(1): 21-31.
32. Liu Y, Wu Q, Cui H, Li Q, Zhao Y, Luo J, Liu Q, Sun X, Tang B, Zhang L, Dai Y, Li N (2008) Expression of EGFP and NPTII protein is not associated with organ abnormalities in deceased transgenic cloned cattle. Cloning Stem Cells 10(4): 421-428. doi: 10. 1089/clo. 2008. 0015.
33. Maga EA, Murray JD (2010) Welfare applications of genetically engineered animals for use in agriculture. J Anim Sci 88(4): 1588-1591. doi: 10. 2527/jas. 2010-2828.
34. Mertens M (2008) Assessment of environmental impacts of genetically modified plants: implementation of the biosafety protocol development of assessment bases fkz 201 67 430/07. BfN-Skripten (Bundesamt fur Naturschutz) (217): 234.
35. Montagne P, Cuilliere ML, Mole C, Bene MC, Faure G (2001) Changes in lactoferrin and lysozyme levels in human milk during the first twelve weeks of lactation. Adv Exp Med Biol 501: 241-247.
36. Muyzer G, Dewaal EC, Uitterlinden AG (1993) Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16s rRNA. Appl Environ Microbiol 59(3): 695-700.
37. Nechvatal JM, Ram JL, Basson MD, Namprachan P, Niec SR, Badsha KZ, Matherly LH, Majumdar APN, Kato I (2008) Fecal collection, ambient preservation, and DNA extraction for PCR amplification of bacterial and human markers from human feces. J Microbiol Methods 72(2): 124-132. doi: 10. 1016/j. mimet. 2007. 11. 007.
38. Nicholson JK, Holmes E, Wilson ID (2005) Gut microorganisms, mammalian metabolism and personalized health care. Nat Rev Microbiol 3(5): 431-438. doi: 10. 1038/nrmicro1152.
39. Nielsen KM, Bones AM, Smalla K, van Elsas JD (1998) Horizontal gene transfer from transgenic plants to terrestrial bacteria-a rare event? FEMS Microbiol Rev 22(2): 79-103.
40. NRC (2002) Animal biotechnology: science based concerns. National Academies Press, Washington, DC.
41. Piccinini R, Binda E, Belotti M, Casirani G, Zecconi A (2005) Comparison of blood and milk non-specific immune parameters in heifers after calving in relation to udder health. Vet Res 36(5-6): 747-757. doi: 10. 1051/vetres: 2005030.
42. Pontiroli A, Rizzi A, Simonet P, Daffonchio D, Vogel TM, Monier J-M (2009) Visual evidence of horizontal gene transfer between plants and bacteria in the phytosphere of transplastomic tobacco. Appl Environ Microbiol 75(10): 3314-3322.
43. Raybould A, Tuttle A, Shore S, Stone T (2009) Environmental risk assessments for transgenic crops producing output trait enzymes. Transgenic Res.
44. Ren NQ, Xing DF, Rittmann BE, Zhao LH, Xie TH, Zhao X (2007) Microbial community structure of ethanol type fermentation in bio-hydrogen production. Environ Microbiol 9(5): 1112-1125. doi: 10. 1111/j. 1462-2920. 2006. 01234. x.
45. Smalla K, Oros-Sichler M, Milling A, Heuer H, Baumgarte S, Becker R, Neuber G, Kropf S, Ulrich A, Tebbe CC (2007) Bacterial diversity of soils assessed by DGGE, T-RFLP and SSCP fingerprints of pcr-amplified 16s rRNA gene fragments: do the different methods provide similar results? J Microbiol Methods 69(3): 470-479. doi: 10. 1016/j. mimet. 2007. 02. 014.
46. Tamura K, Dudley J, Nei M, Kumar S (2007) Mega4: molecular evolutionary genetics analysis (mega) software version 4. 0. Mol Biol Evol 24(8): 1596-1599. doi: 10. 1093/molbev/msm092.
47. Thompson JR, Marcelino LA, Polz MF (2002) Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by 'reconditioning PCR'. Nucleic Acids Res 30(9): 2083-2088.
48. Tong J, Wei H, Liu X, Hu W, Bi M, Wang Y, Li Q, Li N (2010) Production of recombinant human lysozyme in the milk of transgenic pigs. Transgenic Res. doi: 10. 1007/s11248-010-9409-2.
49. van Reenen CG (2009) Assessing the welfare of transgenic farm animals. In: Engelhard M, Hagen K, Boysen M (eds) Genetic engineering in livestock: New applications and interdisciplinary perspectives, vol 34. Ethics of science and technology assessment. pp 119-143. doi: 10. 1007/978-3-540-85843-0_6.
50. van Reenen CG, Meuwissen THE, Hopster H, Oldenbroek K, Kruip TAM, Blokhuis HJ (2001) Transgenesis may affect farm animal welfare: a case for systematic risk assessment. J Anim Sci 79(7): 1763-1779.
51. Wang J, Yang P, Tang B, Sun X, Zhang R, Guo C, Gong G, Liu Y, Li R, Zhang L, Dai Y, Li N (2008) Expression and characterization of bioactive recombinant human alpha-lactalbumin in the milk of transgenic cloned cows. J Dairy Sci 91(12): 4466-4476. doi: 10. 3168/jds. 2008-1189.
52. Xing DF, Cheng SA, Logan BE, Regan JM (2010) Isolation of the exoelectrogenic denitrifying bacterium comamonas denitrificans based on dilution to extinction. Appl Microbiol Biotechnol 85(5): 1575-1587. doi: 10. 1007/s00253-009-2240-0.
53. Yang P, Wang J, Gong G, Sun X, Zhang R, Du Z, Liu Y, Li R, Ding F, Tang B, Dai Y, Li N (2008) Cattle mammary bioreactor generated by a novel procedure of transgenic cloning for large-scale production of functional human lactoferrin. PLoS One 3(10): e3453. doi: 10. 1371/journal. pone. 0003453.
54. Yu SY, Cao GS, Fan BL, Li N, Duan YL, Dai YP, Ll Wang (2004) The cloning of human α-lactalbumin gene and high-level expression in milk of transgenic mice. Prog Biochem Biophys 31(3): 244-248.
55. Yu Z, Meng Q, Yu H, Fan B, Yu S, Fei J, Wang L, Dai Y, Li N (2006) Expression and bioactivity of recombinant human lysozyme in the milk of transgenic mice. J Dairy Sci 89(8): 2911-2918. doi: 10. 3168/jds. S0022-0302(06)72563-2.
56. Zoetendal EG, Collier CT, Koike S, Mackie RI, Gaskins HR (2004) Molecular ecological analysis of the gastrointestinal microbiota: a review. J Nutr 134(2): 465-472.