Kinetic and Sequence-structure-function analysis of known Lina variants with different hexachlorocyclohexane isomers

PLoS ONE

Volumn 6, Issue 9, 2011, Pages

  Sharma, Pooja ^a   Pandey, Rinku ^b   Kumari, Kirti ^a   Pandey, Gunjan ^b   Jackson, Colin J ^c   Russell, Robyn J ^b   Oakeshott, John G ^b   Lal, Rup ^a  

^a UNIVERSITY OF DELHI   (India)

^b CSIRO   (Australia)

^c AUSTRALIAN NATIONAL UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA HEXACHLOROCYCLOHEXANE; DELTA HEXACHLOROCYCLOHEXANE; HEXACHLOROCYCLOHEXANE; LINDANE; UNCLASSIFIED DRUG; ANTICONVULSIVE AGENT; BACTERIAL PROTEIN; LYASE;

ARTICLE; BACTERIAL GENE; BINDING AFFINITY; BIOREMEDIATION; CODON; CONTROLLED STUDY; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME KINETICS; ESCHERICHIA COLI; GENE SEQUENCE; GENETIC VARIABILITY; HYDROPHOBICITY; LINA GENE; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN METABOLISM; PROTEIN PURIFICATION; STEREOSPECIFICITY; STRUCTURE ACTIVITY RELATION; CHEMICAL MODEL; CHEMISTRY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; STEREOISOMERISM;

ANTICONVULSANTS; BACTERIAL PROTEINS; BIODEGRADATION, ENVIRONMENTAL; ESCHERICHIA COLI; GENETIC VARIATION; KINETICS; LINDANE; LYASES; MODELS, CHEMICAL; STEREOISOMERISM;

EID: 80052851023     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0025128     Document Type: Article

References (16)

1. Slade RE, (1945) The gamma isomer of hexachlorocyclohexane (gammexane). An insecticide with outstanding properties. Chem Ind 40: 314-319.
2. Lal R, Pandey G, Sharma P, Kumari K, Malhotra S, et al. (2010) Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation. Microbiol Mol Biol Rev 74: 58-80.
3. Phillips TM, Lee H, Trevors JT, Seech AG, (2006) Full-scale in situ bioremediation of hexachlorocyclohexane-contaminated soil. J Chem Technol Biotechnol 81: 289-298.
4. Rubinos DA, Villasuso R, Muniategui S, Barral MT, Diaz Fierros F, (2007) Using the landfarming technique to remediate soils contaminated with hexachlorocyclohexane isomers. Water Air Soil Pollut 181: 385-399.
5. Okai M, Kubota K, Fukuda M, Nagata Y, Nagata K, et al. (2010) Crystal structure of γ-hexachlorocyclohexane dehydrochlorinase LinA from Sphingobium japonicum UT26. J Mol Biol 403: 260-269.
6. Nagata Y, Mori K, Takagi M, Murzin AG, Damborsky J, (2001) Identification of protein fold and catalytic residues of γ-hexachlorocyclohexane dehydrochlorinase LinA. Proteins 45: 471-477.
7. Brittain DRB, Pandey R, Kumari K, Sharma P, Pandey G, et al. (2011) Competing SN2 and E2 reaction pathways for hexachlorocyclohexane degradation in the gas phase, solution and enzymes. Chem Commun 47: 976-978.
8. Trantirek L, Hynkova K, Nagata Y, Murzin A, Ansorgova A, et al. (2001) Reaction mechanism and stereochemistry of γ-hexachlorocyclohexane dehydrochlorinase LinA. J Biol Chem 276: 7734-7740.
9. Suar M, Hauser A, Poiger T, Buser HR, Muller MD, et al. (2005) Enantioselective transformation of alpha-hexachlorocyclohexane by the dehydrochlorinases LinA1 and LinA2 from the soil bacterium Sphingomonas paucimobilis B90A. Appl Environ Microbiol 71: 8514-8518.
10. Sambrook J, Fritsch EF, Maniatis T, (1989) Molecular Cloning: A Laboratory Manual New York Cold Spring Harbor Laboratory Press.
11. Guerois R, Nielsen JE, Serrano L, (2002) Predicting changes in the stability of proteins and protein complexes: a study of more than 1000 mutations. J Mol Biol 320: 369-387.
12. Schymkowitz J, Borg J, Stricher F, Nys R, Rousseau F, et al. (2005) The FoldX web server: an online force field. Nucleic Acids Res 33 (Suppl 2): W382-388.
13. Lal R, Dogra C, Malhotra S, Sharma P, Pal R, (2006) Diversity, distribution and divergence of lin genes in hexachlorocyclohexane-degrading sphingomonads. Trends Biotechnol 24: 121-130.
14. Bershtein S, Segal M, Bekerman R, Tokuriki N, Tawfik DS, (2006) Robustness-epistasis link shapes the fitness landscape of a randomly drifting protein. Nature 444: 929-932.
15. Nagata Y, Miyauchi K, Takagi M, (1999) Complete analysis of genes and enzymes for γ-hexachlorocyclohexane degradation in Sphingomonas paucimobilis UT26. J Ind Microbiol Biotechnol 23: 380-390.
16. Nagata Y, Hatta T, Imai R, Kimbara K, Fukuda M, et al. (1993) Purification and characterization of γ-hexachlorocyclohexane (γ-HCH) dehydrochlorinase (LinA) from Pseudomonas paucimobilis. Biosci Biotech Biochem 59: 1582-1583.